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Journal of ZheJiang University(Medical Science)  2013, Vol. 42 Issue (2): 149-155    DOI: 10.3785/j.issn.1008-9292.2013.02.004
Multidrug resistance of enteric bacilli and its relation to structure and molecular evolution of variable region in resistance-related class-Ⅰ integron
WANG Huan1, BAO Qi-Yu2, SUN Ai-Hua3, ZHAO Jin-Fang4, GE Yu-Mei5, YAN Jie5
1.Department of Laboratory Medicine,The People′s Hospital of Zhejiang Province,Hangzhou 310014,China; 2.School of Laboratory Medical Science and Life Science,Wenzhou Medical College,Wenzhou 325035,China; 3.Zhejiang Medical College,Hangzhou 310053,China; 4.Department of Laboratory Medicine,The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,China; 5.Department of Medical Microbiology and Parasitology,Zhejiang University School of Medicine,Hangzhou 310058,China
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Objective: To investigate the drug resistance of enteric bacilli and its relation to the drug resistance gene cassette in the variable region and molecular evolution of class-Ⅰ integron.
Methods: K-B assay was applied to measure the drug resistance of E.coli,E.cloacae and A.baumannii isolated against twelve antibiotics.The class-Ⅰ integron and drug resistance gene cassettes in the variable region of the integron were detected by PCR and sequencing of amplification products.The molecular evolution of drug resistance genes in the class-Ⅰ integrons was analyzed using Clustal X and MEGA software.
Results: 54.2%-100% of A.baumannii isolates were resistant to the penicillin and cephem antibiotics,while E.coli and E.cloacae isolates had resistance rates of 41.6%-62.5% to cephem antibiotics.62.5%(15/24) of E.coli,67.9%(19/28) of E.cloacae and 83.3%(20/24) of A.baumannii isolates were positive for class-Ⅰ integrons.81.5%(44/54) of class-Ⅰ integrons showed 4 different single band spectrums and the other class-Ⅰ integrons displayed 3 different double band spectrums.In the drug resistance gene cassettes in variable regions of class-Ⅰ integrons there were 7 types in 4 groups of drug resistance genes,including aac(6′), sad(3″),aad(2″),cat(4′) and dfr(types 7,A13 and 15), which induced the resistance to aminoglycosides and sulfamido antibiotics and chloromycin.The class-Ⅰ integrons in the isolates might be divided into 4 molecular evolution groups according to the diversity of dihydrofolate reductase encoding gene sequences.
Conclusion: The enteric bacilli have a high drug resistance and frequently carry class-Ⅰ integrons with 7 drug resistance gene cassettes which present 4 different evolutionary pathways.

Key wordsEnterobacter/drug effects      Acinetobacter baumannii/drug effects      Escherichia/drug effects      Drug resistance,bacterial      Enteric bacilli      Drug resistance      Integron      Drug resistance gene cassette      Evolution     
Received: 28 December 2012      Published: 25 March 2013
CLC:  R 378.2  
Cite this article:

WANG Huan, BAO Qi-Yu, SUN Ai-Hua, ZHAO Jin-Fang, GE Yu-Mei, YAN Jie. Multidrug resistance of enteric bacilli and its relation to structure and molecular evolution of variable region in resistance-related class-Ⅰ integron. Journal of ZheJiang University(Medical Science), 2013, 42(2): 149-155.

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方法:采用K-B法检测大肠埃希菌、阴沟肠杆菌和鲍曼不动杆菌对12种药物的敏感性。采用PCR及其产物测序检测上述菌株中Ⅰ类整合子携带率及可变区耐药基因盒。采用Clustal X和MEGA软件分析Ⅰ类整合子基因盒中耐药基因分子进化关系。

关键词: 肠杆菌属/药物作用,  鲍氏不动杆菌/药物作用,  埃希氏菌属/药物作用,  抗药性,  细菌,  肠道杆菌,  耐药,  整合子,  耐药基因盒,  进化 

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