Please wait a minute...
浙江大学学报（医学版）
Journal of ZheJiang University(Medical Science)  2011, Vol. 40 Issue (4): 365-373    DOI: 10.3785/j.issn.1008-9292.2011.04.004
    

Anti-tumor activity of components isolated from purple sweet potato polysaccharides
ZHAO Jing1,RUAN Hong1,GAO Qiu-ping2,LI Meng-ya1,TAO Ye-qi1,ZHENG Ying1
1.School of Medicine and Life Science,Zhejiang University City College,Hangzhou 310015,China; 2.College of Life Sciences,Zhejiang University,Hangzhou 310058,China
Download: HTML ( )    PDF(3081KB)
Export: BibTeX | EndNote (RIS)      

Abstract  Objective： To isolate and purify components from polysaccharides of purple sweet potato (PPSP) and to test their anti-tumor activity.
Methods： DEAE-Cellulose and CM-Cellulose exchange chromatography were applied to separate components of PPSP. The anti-tumor activities of each component were measured by MTT assay on Hela and HepG2 cells and their monosaccharide composition were analyzed by TLC chromatography,followed by infrared spectroscopy studies.
Results： Through weak anion exchange chromatography and gradient elution by sodium chloride solution,four components were separated and named as PPSP,PPSPⅡ,PPSPⅢ and PPSPⅣ，respectively. MTT tests showed that PPSPⅡ and PPSPⅢ inhibited Hela and HepG2 tumor cells in a certain extent. The structural analysis revealed that PPSPI was mainly composed of glucose and galactose,PPSPⅡwas composed of glucose and had a typical absorption peak of β-D-glucose chitosan pyranose,PPSPⅢ was a glycoprotein showing a protein absorption peak.
Conclusion： Four components were separated from PPSP successfully,among which PPSPⅡ and PPSP Ⅲ shows anti-tumor activities on Hela and HepG2 cells in vitro.

Key words： Ipomoea batatas/chem      Polysaccharides/isol      Antineoplastic agents/pharmacol      Purple sweet potato      Polysaccharide      Isolation      Chromatography      Component analysis      Anti-tumor     
Published: 25 July 2010
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
Cite this article:

.
Anti-tumor activity of components isolated from purple sweet potato polysaccharides. Journal of ZheJiang University(Medical Science), 2011, 40(4): 365-373.

URL:

https://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2011.04.004     OR     https://www.zjujournals.com/med/Y2011/V40/I4/365


紫心甘薯多糖的分离及组分抑癌活性研究

目的：分离紫心甘薯的多糖（Polysaccharide of purple sweet potato，PPSP）组分，并对其组分的抑癌活性进行初步研究。
方法：采用DEAE-Cellulose和CM-Cellulose离子交换柱分离紫心甘薯多糖，收集分离PPSP组分；MTT法检测PPSP各组分对肿瘤细胞的体外抑制活性；薄层层析法分析PPSP各组分的单糖组成；利用傅里叶红外光谱技术检测PPSP组分的结构特征。
结果：选用弱阴离子交换柱DEAE-Cellulose分离，并经NaCl溶液梯度分段洗脱，得到4个紫心甘薯多糖组分，分别命名为PPSPⅠ、PPSPⅡ、PPSP Ⅲ和PPSP Ⅳ；观察PPSPⅠ、PPSPⅡ、PPSP Ⅲ对Hela和HepG2肿瘤细胞的抑制作用，检测发现PPSPⅡ、PPSP Ⅲ组分有一定的抑癌活性。结构分析结果表明，PPSPⅠ主要由葡萄糖和半乳糖这两种单糖组成；PPSP Ⅱ由葡萄糖组成，并具有β-D-葡萄吡喃聚糖的红外特征吸收峰；PPSP Ⅲ具有蛋白吸收峰。
结论：成功分离得到PPSP的4种多糖组分，其中检测到PPSP Ⅱ、PPSP Ⅲ组分对Hela和HepG2肿瘤细胞具有一定的体外抑制作用。推测PPSP Ⅱ、PPSP Ⅲ组分抑癌活性可能与其含有β-D-葡萄吡喃聚糖或糖蛋白的结构有关。

关键词： 甘薯/化学,  多糖类/分离和提纯,  抗肿瘤药/药理学,  紫心甘薯,  多糖,  分离,  层析,  组分分析,  抗肿瘤 
[1] ZHANG Yi,YUAN Hong. Simultaneous determination of related substances in entecavir film-coated tablet by high performance liquid chromatography[J]. Journal of ZheJiang University(Medical Science), 2021, 50(4): 529-536.
[2] CHENG Lili,ZHU Haixiang,PAN Hongying,HUANG Susu,ZHUANG Yiyu,ZHU Chenping,YUAN Yuhua,ZHU Hongfang,CHEN Huiyin,XU Hongxia. Development and application of quality checklist for the prevention and control of COVID-19 in fever clinic and isolation ward of the general hospital[J]. Journal of ZheJiang University(Medical Science), 2021, 50(1): 74-80.
[3] FENG Yihan,YIN Lei,LIU Yuanrong,CAO Lujing,ZHENG Ning,LI Mingjuan,ZHAN Shuyu. Quantitative determination and optimun extraction technique of nine compounds of Paeoniae Radix Alba[J]. Journal of ZheJiang University(Medical Science), 2020, 49(3): 356-363.
[4] LIU Jingwen,YANG Xinglian,SHEN Kaili,ZENG Linghui,SUN Yan. Chloroxoquinoline inhibits invasion in breast cancer via down-regulating Rho/Rho kinase signaling pathway[J]. Journal of ZheJiang University(Medical Science), 2019, 48(6): 631-637.
[5] YE Peiwu,YU Xiafei,MA Cheng,YANG Wei. Extraction and purification of NUDT9 homology domain of human transient receptor potential melastatin 2 channel[J]. Journal of ZheJiang University(Medical Science), 2019, 48(1): 5-11.
[6] ZHU Min,WU Yunqiu,SHOU Zhangfei. Effects of Niaoduqing granule on urine metabolic profile in chronic renal failure rats[J]. Journal of ZheJiang University(Medical Science), 2018, 47(6): 628-635.
[7] WANG Xiaorong,LU Yunbi,ZHANG Weiping,WEI Erqing,FANG Sanhua. Effects of cysteinyl leukotriene receptors on phagocytosis of mouse microglial cells[J]. Journal of ZheJiang University(Medical Science), 2018, 47(1): 10-18.
[8] LIU Tingting,WANG Lingxiao,YANG Xiaohui,YAO Zhiqing,CAI Huizhen. TLR/NF-κB independent signaling pathway in TNF-α suppression of diabetic MyD88-knockout mice after Lycium barbarum polysaccharides administration[J]. Journal of ZheJiang University(Medical Science), 2018, 47(1): 35-40.
[9] ZHAN Shuyu,RUAN Yu'er,LIU Guoqiang,DING Baoyue,SHAO Qing. Experimental factors affecting in vitro recovery of puerarin in microdialysis[J]. Journal of ZheJiang University(Medical Science), 2018, 47(1): 64-70.
[10] HOU Jiafu,WU Leilei,CAI Yuting,GAO Changjiu,AN Jindan,YU Zhongcheng. Antiendotoxin effect of Jinhuaqingre capsules[J]. Journal of ZheJiang University(Medical Science), 2017, 46(1): 74-79.
[11] MA Tingting,WANG Yi,CHEN Xiaoqian,ZHAO Xiaoping. LC/MS guided approach to discovering nephroprotective substances from Huangkui capsule[J]. Journal of ZheJiang University(Medical Science), 2017, 46(1): 66-73.
[12] SONG Wei, LIU Li, LU Hongzhou. Identification of Mycobacteria isolated from AIDS patients[J]. Journal of ZheJiang University(Medical Science), 2016, 45(3): 243-248.
[13] SU Min, CHEN Jin, BAI Bing, HUANG Yunxiu, WEI Lan, LIU Minyan, CHEN Tingmei. Establishment and preliminary application of detection of Mycobacterium tuberculosis in sputum based on variable number tandem repeat[J]. Journal of ZheJiang University(Medical Science), 2016, 45(1): 61-67.
[14] QIU Li-ping, SUN Wen-jun. Simultaneous quantitative analysis of different ceramide species in cells by high-performance liquid chromatography-tandem mass spectrometry[J]. Journal of ZheJiang University(Medical Science), 2015, 44(4): 429-434.
[15] TAO Shan, LIANG Xin-ying, WANG Yi, WANG Yi. Screening of active compounds with myocardial protective effects from Tongmai Yangxin pill[J]. Journal of ZheJiang University(Medical Science), 2015, 44(2): 145-153.