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Journal of ZheJiang University(Medical Science)  2000, Vol. 29 Issue (2): 63-66    DOI: 10.3785/j.issn.1008-9292.2000.02.006
    
Detection of Intercellular Adhesion Molecule-1 during Pediatric Cardiopulmonary Bypass
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Abstract  

To evaluate the role of serum intercellular adhesion molecule (ICAM-1)changes during pediatric cardiopulmonary bypass (CPB) surgery for congenital heart diseases (CHD)and the clinic significance of monitoring the serum ICAM-1 level. Methods: ICAM-1 levels were measured by double antibodies sandwich ELISA in arterial specimen from 33 CPB cases at various time points,the results were compared with those of 10 non-CPB patients and 30 normal children. Results:Patients with CHD had normal levels of serum ICAM-1 before surgery. The serum ICAM-1 levels in CPB group were significantly higher than those of controls (P〈0. 005). ICAM-1 levels started increasing as early as 30 minutes of CPB began and peaked 24 hours after surgery and stayed up 48 hours after surgery . The serum ICAM-1 levels in patients whose CPB lasted longer than 90 minuets were significantly higher (P<0.05). Conclusion:ICAM-1 showed higher and lasted longer after pediatric CPB surgery. The length of CPB is associated with elevation of ICAM-1. Monitoring of serumICAM-1 ,in certain degree,may help in predicting prognosis and complication after CPB surgery.



Key wordsCell adhesion molecules/blood      Heart defects,congenital/surg      Extracorporeal circulation      Postoperative period      Children     
Published: 25 March 2000
Cite this article:

. Detection of Intercellular Adhesion Molecule-1 during Pediatric Cardiopulmonary Bypass . Journal of ZheJiang University(Medical Science), 2000, 29(2): 63-66.

URL:

https://www.zjujournals.com/med/10.3785/j.issn.1008-9292.2000.02.006     OR     https://www.zjujournals.com/med/Y2000/V29/I2/63


小儿体外循环手术围术期细胞间粘附分子-1的测定

目的:探讨小儿体外循环(CPB)围术期血清细胞间粘附分子-1(ICAM-1)水平变化规律和升高原因及测定的临床意义。方法:采用双抗夹心法酶联吸附试验(ELISA)法分别于手术前、转流后30 min、手术毕、术后2 h、12 h、24 h、48 h共7个时点测定33例先天性心脏病(CHD)患儿;分别于术前,术中及术后2 h测定10例非体外循环普胸手术患儿及30例健康体检患儿ICAM-1水平。结果:CHD术前ICAM-1无变化;CPB术后ICAM-1水平较非CPB明显升高,P<0.005;血清ICAM-1值于CPB 30 min后开始上升,至24 h达到高峰后开始下降,48 h尚未降至术前水平;CPB时间≥90 min组ICAM-1显著升高,P<0.05。结论:小儿CPB心脏直视手术后血清ICAM-1升高明显,CPB时间是ICAM-1升高的主要原因,ICAM-1测定对预防及早期发现并发症有一定的临床意义。


关键词: 细胞粘着分子/血液,  心脏缺损,  先天性/外科学,  体外循环,  手术后期间,  儿童 
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