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J Zhejiang Univ (Med Sci)  2022, Vol. 51 Issue (1): 47-52    DOI: 10.3724/zdxbyxb-2021-0282
    
Genotypes of Bordetella pertussis isolated from infants in Xi’an and Shanghai
ZHANG Juansheng1,LIN Chen2,*(),CHANG Ling1,WANG Xiaoqiang1,WEI Xiaoguang1,LI Hao1
1. Xi’an Center for Disease Control and Prevention, Xi’an 710054, China;
2. Shanghai Pudong New Area Center for Disease Control and Prevention, Shanghai 200136, China
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Abstract  

Objective: To compare the genotypes of Bordetella pertussis isolated from infants in Xi’an and Shanghai. Methods: Samples were collected by nasopharyngeal swab from infants aged <1?year hospitalized with suspected pertussis in Xi’an and Shanghai during 2018 and 2019. Bordetella pertussis was isolated, and multilocus antigen sequence typing (MAST) and multilocus variable-number tandem repeat analysis (MLVA) were used to analyse the genotypes. Results: A total of 1200 samples were collected from infants suspected of pertussis and 60 strains of Bordetella pertussis were isolated, including 34 strains in Xi’an and 26 strains in Shanghai. There were significant differences in the MAST types between Xi’an and Shanghai (χ2=18.642, P<0.01); theprn1/ptxP1/ptxA1/fim3-1/fim2-1 strains dominated in Xi’an (32/34, 94.12%), while the dominated MAST types in Shanghai were prn1/ptxP1/ptxA1/fim3-1/fim2-1 (13/26, 50.00%) and prn2/ptxP3/ptxA1/fim3-1/fim2-1 (11/26, 42.31%). The composition of MLVA type of pertussis strains was also significantly different between Xi’an and Shanghai (χ2=15.866, P<0.01); the MT195 (13/34, 38.24%), MT55 (10/34, 29.41%) and MT104 (9/34, 26.47%) strains dominated in Xi’an, while the MT27 (12/26, 46.15%) strain was most common in Shanghai.Conclusion: There are differences in molecular types of Bordetella pertussis isolated from infants with suspected persussis in Xi’an and Shanghai, indicating that further monitoring of Bordetella pertussis is necessary for better understanding the pathogen evolution in China.



Key wordsPertussis      Bordetella pertussis      Genotype      Multilocus antigen sequence typing      Multilocus variable-number tandem repeat analysis     
Received: 15 September 2021      Published: 17 May 2022
CLC:  R516.6  
Corresponding Authors: LIN Chen     E-mail: 247372205@qq.com
Cite this article:

ZHANG Juansheng,LIN Chen,CHANG Ling,WANG Xiaoqiang,WEI Xiaoguang,LI Hao. Genotypes of Bordetella pertussis isolated from infants in Xi’an and Shanghai. J Zhejiang Univ (Med Sci), 2022, 51(1): 47-52.

URL:

https://www.zjujournals.com/med/10.3724/zdxbyxb-2021-0282     OR     https://www.zjujournals.com/med/Y2022/V51/I1/47


西安市与上海市婴儿感染百日咳鲍特菌基因型比较

目的:探讨西安市与上海市临床分离百日咳鲍特菌的基因型特征。方法:收集2018—2019年在西安市和上海市哨点监测医院和社区采集的1岁以下疑似百日咳患儿的鼻咽拭子,经分离培养所得百日咳鲍特菌采用多位点抗原序列分型(MAST)和多位点可变数目串联重复序列分析(MLVA)方法分析其基因型。结果:共采集到疑似百日咳患儿鼻咽拭子1200份,经分离培养得到百日咳鲍特菌60株,其中西安市34株,上海市26株。西安市百日咳鲍特菌的主要MAST型别为prn1/ptxP1/ptxA1/fim3-1/fim2-1(32/34,94.12%),而上海市百日咳鲍特菌的主要MAST型别为prn1/ptxP1/ptxA1/fim3-1/fim2-1(13/26,50.00%)和prn2/ptxP3/ptxA1/fim3-1/fim2-1(11/26,42.31%),两市百日咳鲍特菌MAST型别分布差异有统计学意义(χ2=18.642,P<0.01)。西安市百日咳鲍特菌以MT195(13/34,38.24%)、MT55(10/34,29.41%)、MT104(9/34,26.47%)型别居多,而上海市百日咳鲍特菌以MT27型为主(12/26,46.15%),两市百日咳鲍特菌MLVA型别分布差异有统计学意义(χ2=15.866,P<0.01)。结论:西安市与上海市临床分离婴儿感染百日咳鲍特菌基因型存在差异,应进一步加强中国百日咳鲍特菌基因型演变的监测。


关键词: 百日咳,  百日咳鲍特菌,  基因型,  多位点抗原序列分型,  多位点可变数目串联重复序列分析 

地市

n

疫苗接种情况

疫苗类型

未到接种月龄

未全程免疫

全程免疫

西安市

34

16(47.06)

11(32.35)

7(20.59)

无细胞百白破疫苗(武汉生物制品研究所有限责任公司)

上海市

26

10(38.46)

11(42.31)

5(19.23)

无细胞百白破疫苗(武汉生物制品研究所有限责任公司)、五联疫苗(法国巴斯德生物制药有限公司)、四联疫苗(北京民海生物科技有限公司)

Table 1 Status of pertussis vaccination of 60 children with infection

MAST型别

西安市

上海市

prn1/ptxP1/ptxA1/fim3-1/fim2-1

32(94.12)

13(50.00)

prn9/ptxP3/ptxA1/fim3-1/fim2-1

1(2.94)

0(0.00)

prn2/ptxP3/ptxA1/fim3-1/fim2-1

1(2.94)

11(42.31)

prn1/ptxP1/ptxA1/fim3-4/fim2-1

0(0.00)

1(3.85)

prn4/ptxP1/ptxA1/fim3-1/fim2-1

0(0.00)

1(3.85)

Table 2 Comparison of MAST results of isolates in Xi’an and Shanghai

MLVA型别

西安市

上海市

MT195

13 (38.24)

4(15.38)

MT104

9(26.47)

3(11.54)

MT55

10(29.41)

6(23.08)

MT27

2(5.88)

12(46.15)

MT10

0(0.00)

1(3.85)

Table 3 Comparison of MLVA results of isolates in Xi’an and Shanghai
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