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J Zhejiang Univ (Med Sci)  2021, Vol. 50 Issue (3): 361-368    DOI: 10.3724/zdxbyxb-2021-0007
    
Effect of electro-acupuncture therapy on limb spasm and excitability of motor neurons in stroke rats
LIU Junxia1,*,ZHAO Guigui1,NIU Yan2,GAN Ting1,YAN Zhenyu1,ZHANG Yasu3
1. Medical College of Zhengzhou Institute of Technology, Xinzheng 451100, Henan Province, China;
2. Department of Internal Medicine, Xinzheng Hospital of Traditional Chinese Medicine, Xinzheng 451100, Henan Province, China;
3. Rehabilitation of Traditional Chinese Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450000, China
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Abstract  

Objective:To investigate the effect of electro-acupuncture therapy on limb spasm and excitability of motor neurons in stroke rats. Methods:Ischemic stroke model was induced with middle cerebral artery embolization in SD rats. Thirty-three modeled rats were randomly divided into model group, electro-acupuncture group, and baclofen group with 11 rats in each group, and another 10 rats were taken as sham operation group. The electro-acupuncture group and the baclofen group were treated with electro-acupuncture and baclofen tablets respectively. The model group and the sham operation group had no intervention. The neural function was evaluated with Bederson’s scale and balance beam test; the muscle tension was measured with electrophysiography; the pathological changes of brain tissue was examined with HE staining; the content of glutamic acid (Glu) and γ-aminobutyric acid (GABA) in rat cerebral cortex was analyze with enzyme linked immunosorbent assay (ELISA) method, the expression of metabotropic glutamate receptor 1a (Grm1a) and γ-aminobutyric acid type B receptor subunit 1 (Gabbr1) mRNA were detected with RT-qPCR. Results:Compared with the model group, the neurological function scores of the electro-acupuncture group and the baclofen group showed a downward trend at d7 after operation (all P>0.05), and the neurological function scores of the electro-acupuncture group and the baclofen group were significantly decreased at d12 after the operation (allP<0.05). Compared with sham operation group, the electrophysiological results of model group, electro-acupuncture group and baclofen group were significantly lower (allP<0.05), and there was no statistical difference in the electrophysiological results of the model group, electro-acupuncture group and baclofen group at d7 after operation (allP>0.05). Compared with the model group, the electrophysiological results of the electro-acupuncture group and baclofen group were significantly increased 12?d after operation (allP<0.05). The results of HE staining showed that there was no cell edema and degeneration in the sham operation group, no pyknosis of the nucleus, and no bleeding in the interstitium. Cell edema and degeneration and mesenchymal congestion appeared in the model group. Compared with the model group, the cytoplasmic edema and degeneration and the interstitial bleeding in the electroacupuncture group and the baclofen group were reduced. Compared with sham operation group, the Glu content and the relative expression ofGrm1a mRNA was increased in the model group, electro-acupuncture group and baclofen group, while the GABA content and the relative expression of Gabbr1 mRNA decreased (all P<0.05). Compared with model group, the Glu content and the relative expression ofGrm1a mRNA in the electro-acupuncture group and baclofen group decreased, and the GABA content and relative expression of Gabbr1 mRNA increased (all P<0.05).Conclusion:Electro-acupuncture may improve limb spasm after stroke through regulating the expression of Glu and GABA in the cerebral cortex and the excitability of motor neurons in rats.



Key wordsElectro-acupuncture therapy      Stroke      Spasm      Motor neuron      Glutamic acid      γ-aminobutyric acid      SD rat     
Received: 30 September 2020      Published: 16 August 2021
CLC:  R743.3  
  R245  
Corresponding Authors: LIU Junxia   
Cite this article:

LIU Junxia,ZHAO Guigui,NIU Yan,GAN Ting,YAN Zhenyu,ZHANG Yasu. Effect of electro-acupuncture therapy on limb spasm and excitability of motor neurons in stroke rats. J Zhejiang Univ (Med Sci), 2021, 50(3): 361-368.

URL:

http://www.zjujournals.com/med/10.3724/zdxbyxb-2021-0007     OR     http://www.zjujournals.com/med/Y2021/V50/I3/361


电针疗法对脑卒中大鼠肢体痉挛的改善作用

目的:探讨电针疗法对脑卒中大鼠肢体痉挛的改善作用及对运动神经元兴奋性的影响。方法:随机选取 45 只大鼠建立缺血性脑卒中大鼠模型,建模成功大鼠 33 只,随机分为模型对照组、电针治疗组、巴氯芬组,各 11 只,另设手术对照组(10 只)。电针治疗组和巴氯芬组分别用电针疗法和巴氯芬干预,模型对照组和手术对照组不干预,仅抓取固定。采用 Bederson 神经功能评分和平衡木行走实验评价神经功能;电生理描记法检测肌张力;苏木精--伊红(HE) 染色检测脑组织病理学变化;酶联免疫吸附试验法分析大鼠大脑皮质中谷氨酸(Glu)、γ-氨基丁酸(GABA)的含量;实时逆转录 聚合酶链反应(PCR) 检测代谢型谷氨酸受体 1a(Grm1a)、γ-氨基丁酸 B 型受体 1(Gabbr1)信使RNA(mRNA) 相对表达量。结果:与模型对照组比较,术后第 7 天电针治疗组、巴氯芬组神经功能评分有下降趋势,但差异均无统计学意义(均P>0.05);术后第 12 天电针治疗组和巴氯芬组神经功能评分均下降(均P<0.05)。术后第 7 天,与手术对照组比较,模型对照组、电针治疗组和巴氯芬组电生理描记结果均降低(均P<0.05),模型对照组、电针治疗组和巴氯芬组电生理描记结果差异均无统计学意义(均P>0.05);术后第 12 天,与模型对照组比较,电针治疗组和巴氯芬组电生理描记结果均升高(均P<0.05)。手术对照组无细胞水肿及变性,细胞核无固缩,间质未见出血;模型对照组出现细胞水肿变性、间质充血;电针治疗组和巴氯芬组与模型对照组比较细胞质水肿变性和间质出血均减轻。与手术对照组比较,模型对照组、电针治疗组和巴氯芬组 Glu 含量、Grm1amRNA 相对表达量均增加,GABA 含量、Gabbr1 mRNA 相对表达量均减少(均P<0.05);与模型对照组比较,电针治疗组和巴氯芬组 Glu 含量、Grm1a mRNA 相对表达量均减少,GABA 含量、Gabbr1 mRNA 相对表达量均增加(均P<0.05)。结论:电针疗法可能通过调节大脑皮质中 Glu 和 GABA 表达,调节运动神经元兴奋性,改善脑卒中肢体痉挛状态。


关键词: 电针疗法,  脑卒中,  痉挛,  运动神经元,  谷氨酸,  γ-氨基丁酸,  SD大鼠 

组别

n

第 1 天

第 7 天

第 12 天

手术对照组

10

0.20±0.01

0.20±0.00

0.10±0.00

模型对照组

11

2.18±0.65*

2.01±0.59*

1.78±0.43*

电针治疗组

11

1.92±0.54*

1.73±0.45*

0.64±0.08*#

巴氯芬组

11

1.89±0.52*

1.72±0.46*

0.62±0.07*#

F

61.202

66.020

161.191

P

<0.01

<0.01

<0.01

Table 1 Bederson nerve function score in each group

组别

n

第 1 天

第 7 天

第 12 天

手术对照组

10

0.30±0.01

0.20±0.00

0.20±0.01

模型对照组

11

4.00±1.12*

3.34±0.98*

2.85±0.76*

电针治疗组

11

3.65±0.87*

3.14±0.86*

1.81±0.49*#

巴氯芬组

11

3.63±0.78*

3.12±0.77*

1.79±0.48*#

F

78.751

65.357

73.219

P

< 0.01

< 0.01

< 0.01

Table 2 Balance beam walking scores in each group

组别

n

第 7 天

第 12 天

手术对照组

10

7.41±1.17

7.40±1.27

模型对照组

11

4.72±0.61*

4.91±0.57*

电针治疗组

11

4.66±0.58*

6.58±0.75*#

巴氯芬组

11

4.68±0.52*

6.60±0.72*#

F

56.464

33.059

P

< 0.01

< 0.01

Table 3 Electrophysiological tracing results in each group
Figure 1 Histopathological changes of brain stem in each group

组别

n

Glu(mg/L)

GABA(μmol/L)

手术对照组

10

9.96±1.12

6.62±0.71

模型对照组

11

15.50±1.72*

4.14±0.51*

电针治疗组

11

10.21±1.22*#

5.42±0.62*#

巴氯芬组

11

10.20±1.13*#

5.41±0.61*#

F

85.690

53.539

P

< 0.01

< 0.01

Table 4 Glutamic acid and γ-aminobutyric acid content in each group

组别

n

Grm1a

Gabbr1

手术对照组

10

0.30±0.07

0.61±0.09

模型对照组

11

0.57±0.09*

0.29±0.15*

电针治疗组

11

0.38±0.08*#

0.42±0.08*#

巴氯芬组

11

0.37±0.04*#

0.43±0.05*#

F

48.334

28.772

P

< 0.01

< 0.01

Table 5 mRNA expression of and in each group
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