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浙江大学学报（医学版）
浙江大学学报（医学版）  2021, Vol. 50 Issue (5): 627-632    DOI: 10.3724/zdxbyxb-2021-0242
原著     
二甲双胍通过抑制内质网应激诱导的细胞凋亡改善结肠炎黏膜上皮屏障损伤
王金钢1,2,陈春晓1,*(),任于晗2,周辛欣1,俞珊2
1.浙江大学附属第一医院消化内科，浙江 杭州 310003
2.浙江大学附属第一医院嵊州分院 嵊州市人民医院消化内科，浙江 嵊州 312400
Metformin alleviates intestinal epithelial barrier damage by inhibiting endoplasmic reticulum stress-induced cell apoptosis in colitis cell model
WANG Jingang1,2,CHEN Chunxiao1,*(),REN Yuhan2,ZHOU Xinxin1,YU Shan2
1. Department of Gastroenterology, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;
2. Department of Gastroenterology, Shengzhou Branch of the First Affiliated Hospital of Zhejiang University School of Medicine, Shengzhou People’s Hospital, Shengzhou 312400, Zhejiang Province, China
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摘要：

目的：探讨二甲双胍对溃疡性结肠炎黏膜上皮屏障损伤的作用及具体机制。方法：用人结肠癌细胞系Caco-2与人单核细胞系THP-1构建结肠炎体外细胞共培养模型，用1?mmol/L二甲双胍作用24?h后，运用流式细胞术检测肠上皮细胞凋亡情况，采用蛋白质印迹法检测紧密连接蛋白和内质网应激相关蛋白的表达水平。结果：与模型对照组比较，二甲双胍组细胞凋亡率从（14.22±2.34）%下降至（9.88±0.61）%（t=3.119，P<0.05），紧密连接蛋白1和密封蛋白1相对表达量增加（t=5.172和3.546，均P<0.05），内质网分子伴侣葡萄糖调节蛋白（GRP）78和内质网应激诱导的凋亡相关分子C/EBP同源蛋白（CHOP）、胱天蛋白酶（caspase）-12的蛋白表达水平下降（均P<0.05），蛋白激酶R样内质网激酶（PERK）和真核生物起始因子2α（eIF2α）的磷酸化水平下降（均P<0.05）。结论：二甲双胍可以通过减轻结肠炎肠上皮细胞的细胞凋亡和增加紧密连接蛋白的表达改善结肠炎肠黏膜上皮屏障损伤，其分子机制可能与抑制内质网应激诱导的细胞凋亡途径有关。
关键词： 结肠炎, 溃疡性;  二甲双胍;  内质网应激;  细胞凋亡;  细胞共培养;  紧密连接蛋白    
Abstract:

Objective:To investigate the effect and mechanism of metformin on intestinal epithelial barrier injury in ulcerative colitis. Methods:A cell model of colitis was established by co-culture of human colon cancer cell line Caco-2 and human monocyte cell line THP-1. The colitis model cells were treated with metformin at concentration of 1?mmol/L for 24?h. Flow cytometry was used to detect Caco-2 cell apoptosis, and Western blotting was used to detect the protein expression of tight junction proteins and endoplasmic reticulum stress-related proteins. Results: After metformin treatment, the apoptosis rate of Caco-2 cells was decreased from (14.22±2.34)% to (9.88±0.61)% (t=3.119, P<0.05), and the expression levels of tight junction protein-1 and claudin-1 increased (t=5.172 and 3.546, both P<0.05). In addition, the expression levels of endoplasmic reticulum-related proteins glucose regulated protein (GRP) 78, C/EBP homologous protein (CHOP) and caspase-12, as well as the phosphorylation level of PRKR-like endoplasmic reticulum kinase (PERK) and eukaryotic translation initiation factor 2α (eIF2α) decreased (allP<0.05).Conclusion:Metformin may alleviate the intestinal epithelial barrier damage in colitis by reducing intestinal epithelial cell apoptosis and increasing the expression of tight junction proteins, which may be associated with the inhibition of endoplasmic reticulum stress-induced apoptotic pathway.
Key words: Colitis, ulcerative    Metformin    Endoplasmic reticulum stress    Apoptosis    Co-culture cell    Tight junction protein
收稿日期: 2021-07-10 出版日期: 2021-12-29
:  R310.17  
通讯作者: 陈春晓     E-mail: 13906523922@126.com
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王金钢
陈春晓
任于晗
周辛欣
俞珊

引用本文:

王金钢,陈春晓,任于晗,周辛欣,俞珊. 二甲双胍通过抑制内质网应激诱导的细胞凋亡改善结肠炎黏膜上皮屏障损伤[J]. 浙江大学学报（医学版）, 2021, 50(5): 627-632.

WANG Jingang,CHEN Chunxiao,REN Yuhan,ZHOU Xinxin,YU Shan. Metformin alleviates intestinal epithelial barrier damage by inhibiting endoplasmic reticulum stress-induced cell apoptosis in colitis cell model. J Zhejiang Univ (Med Sci), 2021, 50(5): 627-632.

链接本文:

https://www.zjujournals.com/med/CN/10.3724/zdxbyxb-2021-0242    或    https://www.zjujournals.com/med/CN/Y2021/V50/I5/627

图 1  模型对照组和二甲双胍组结肠炎肠上皮细胞凋亡流式细胞图
图 2  模型对照组和二甲双胍组紧密连接蛋白1和密封蛋白1表达电泳图
图 3  模型对照组和二甲双胍组内质网应激相关蛋白表达电泳图GRP：葡萄糖调节蛋白；PERK：蛋白激酶R样内质网激酶；eIF2α：真核生物起始因子2α；CHOP：C/EBP同源蛋白；caspase：胱天蛋白酶.

组别

n

GRP78

CHOP

caspase-12

磷酸化PERK/PERK

磷酸化eIF2α/eIF2α

模型对照组

3

1.145±0.048

1.127±0.061

1.173±0.076

0.822±0.012

0.818±0.057

二甲双胍组

3

0.925±0.138

0.760±0.148

0.985±0.045

0.650±0.065

0.568±0.126

t值

—

3.018

4.586

3.691

5.197

3.618

P值

—

<0.05

<0.01

<0.05

<0.05

<0.05
表 1  模型对照组和二甲双胍组内质网应激相关蛋白表达量比较
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