Phosphodiesterase extracted from the venom of Agkistrodon blomhoffii brevicaudus was used to immune BALB/c mice,and their splenic cells were fused with mouse myeloma cell Fo. Ahybridoma cell strain G₈,which could secrete anti-venom-phosphodiesterase antibodies was isolated. The specific antibody in the supernatant of the hybridoma cell cultures and in the ascites of mice innoculated with the hybridoma cells were detected through indirect ELISA,and the titers were 1:128,1:51200 respectively. The results of antigen block test showed that this antibody was specific to the phosphodiesterase of venom. This strain of monoclonal antibody belongs to the mouse IgG2a subtype. Continuous culturing for six months in vitro has revealed the antibody secretion of the cell strain to be stable.