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浙江大学学报（医学版）
浙江大学学报（医学版）  2019, Vol. 48 Issue (6): 638-643    DOI: 10.3785/j.issn.1008-9292.2019.12.08
原著     
京尼平促进牙本质Ⅰ型胶原矿化的实验研究
顾天忆1(),帅旌1,陈超群2,冯剑颖1,*()
1. 浙江中医药大学口腔医学院, 浙江 杭州 310053
2. 浙江大学医学院附属第一医院口腔科, 浙江 杭州 310003
Effect of genipin pretreatment on type Ⅰ collagen mineralization
GU Tianyi1(),SHUAI Jing1,CHEN Chaoqun2,FENG Jianying1,*()
1. School of Stomatology, Zhejiang Chinese Medical University, Hangzhou 310053, China
2. Department of Stomatology, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
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摘要：

目的: 探讨生物交联剂京尼平预处理对牙本质Ⅰ型胶原矿化的影响。方法: 制备Ⅰ型胶原凝胶，分别用质量分数为0.5%的京尼平溶液（京尼平组）和去离子水（对照组）预处理2 h，采用傅里叶变换红外光谱仪检测京尼平是否与胶原纤维发生交联反应。制备单层Ⅰ型胶原模型，按上述分组预处理2 h后矿化处理4 h，应用透射电子显微镜检测两组矿化效果，ImageJ软件定量分析矿化率；应用透射电子显微镜及扫描电子显微镜观察两组脱矿牙本质的成矿效果。结果: 京尼平可与胶原结合；矿化4 h后，京尼平溶液预处理后矿化效果明显优于对照组，矿化率分别为（73.3±5.3）%和（7.4±3.5）%（P < 0.01）；京尼平组较对照组在脱矿牙本质再矿化中效果更好。结论: 京尼平可以促进牙本质Ⅰ型胶原的矿化。
关键词： 京尼平;  Ⅰ型胶原;  牙本质;  再矿化    
Abstract:

Objective: To investigate the effects of bio-crosslinker genipin pretreatment on type Ⅰ collagen mineralization. Methods: Type Ⅰ collagen gels were prepared and pretreated with 0.5wt%genipin (experimental group) and deionized water (control group) for 2 h, respectively. The pretreated products were subjected to Fourier transform infrared spectroscopy (FT-IR). Reconstituted collagen fibrils were pretreated with genipin or deionized water for 2 h and were mineralized for 4 h. The collagen density and mineralization degree were examined with transmission electron microscopy (TEM) and analyzed with ImageJ software. Then scanning electron microscopy (SEM) and TEM were used to observe the mineralization of cross-linked demineralized dentin collagen. Results: FT-IR spectrum showed that the genipin was crosslinked with collagen. TEM observation and ImageJ results showed that after 4 h mineralization, the mineralization effect of 0.5wt% genipin group was significantly better than that of the control group[(73.3±5.3)%vs.(7.4±3.5)%, P < 0.01]. TEM and SEM observation showed that the mineralization rate of type Ⅰ collagen and demineralized dentin pretreated with genipin were significantly faster than that of the control group. Conclusion: The study demonstrates that 0.5 wt% concentration of genipin can significantly promote the mineralization of type Ⅰ collagen.
Key words: Genipin    Type Ⅰ collagen    Dentin    Remineralization
收稿日期: 2019-01-30 出版日期: 2020-01-19
:  R783.1  
基金资助: 浙江省大学生科技创新活动计划(2017R410017);国家级大学生创新训练项目(201810344012)
通讯作者: 冯剑颖     E-mail: 201612201601001@zcmu.edu.cn;twohorsejy@163.com
作者简介: 顾天忆(1996-), 男, 大学生; E-mail:201612201601001@zcmu.edu.cn; https://orcid.org/0000-0001-6926-8054
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引用本文:

顾天忆,帅旌,陈超群,冯剑颖. 京尼平促进牙本质Ⅰ型胶原矿化的实验研究[J]. 浙江大学学报（医学版）, 2019, 48(6): 638-643.

GU Tianyi,SHUAI Jing,CHEN Chaoqun,FENG Jianying. Effect of genipin pretreatment on type Ⅰ collagen mineralization. J Zhejiang Univ (Med Sci), 2019, 48(6): 638-643.

链接本文:

http://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.2019.12.08    或    http://www.zjujournals.com/med/CN/Y2019/V48/I6/638

图 1  Ⅰ型胶原凝胶经0.5 %京尼平溶液预处理2 h后的傅里叶变换红外光谱变化
图 2  对照组与京尼平组单层Ⅰ型胶原矿化4 h后超微结构透射电子显微镜下观察所见
图 3  对照组与京尼平组脱矿牙本质矿化处理2、4 d后表面形貌的扫描电子显微镜下所见
图 4  脱矿牙本质分别矿化处理0、2、4 d后超薄切片的透射电子显微镜下观察所见
图 5  京尼平与胶原的交联反应示意图[17]
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