PCR technique was applied to amplification of three polymorphic short tandem repeats(DYS Ⅰ,DYS Ⅱ, DYS Ⅲ) of DMD gene. The PCR products were electrophoresed on polyacrylamide gel. The results showed that the loci were highly polymorphic,with heterozygosities of 76.7%,86.7% and 43.3% in southeast China. Polymorphism Information Contents(PIC) ranged from 0.659 to 0.697. In a large DMD family haplotype linkage analysis demons-traied that there were no recombination in 10 informative meiosis analysed between the three loci and dystrophin gene. In addition,though the loci were highly polymorphic,in some cases the polymorphic loci were homozygotic and could not provide any information for linkage analysis. It indicated that in a linkage locus.several polymorphic markers should be used,so that more accuracy information would be provided.