The anthors vaccinated 8 volunteers using inactivated EHF vaccines for 3 doses(one dose on 0,7th and 28th days respectively).The vaccines were first infected by M unguiculatus kidney cells with EHFV,strain Z₁₀ and then inactivated by using 0.05% β-propiolactone. On the 28th day after the first dose,the sera from 8 volunteers were detected for anti-EHFV by the RPHIA,ELISA and IFA methods and all were positive.And all the sera were positive provde by the plague reduction neutralization test(PRNT) simultaneouly. Other sera of 3 cases became positive on the 45th day with a positive rate of 87.5% (GMT 40.0).The lymphocyte transformation test was performed by adding ³HTdR into the sera before the vaccination and 7th,14th,28th,3 and 6 months and 1 year after vaccination respectively. The results showed that from 7th day to 3 month affer the first dose,the specific and nonspecific T lymphocyte fransformation was low,which was different to the results before vaccinations. The specific B lymphocyte transformations obviously increased in the 1~1.5 month after vaccination. The P value was <0.05 as compared with that before the vaccination.