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浙江大学学报（医学版）
浙江大学学报（医学版）  2020, Vol. 49 Issue (4): 439-446    DOI: 10.3785/j.issn.1008-9292.2020.04.16
专题报道     
瞬时受体电位通道C与阻塞性睡眠呼吸暂停低通气综合征大鼠心脏和肾脏损害的关系
温雯1(),姚巧玲2,陈玉岚1,*(),李志强3,孙晓靖4,李瑜5,张俊仕1,珠勒皮亚·司马义1,徐新娟1
1. 新疆医科大学第一附属医院高血压科, 新疆维吾尔自治区 乌鲁木齐 830011
2. 新疆医科大学基础医学院生理教研室, 新疆维吾尔自治区 乌鲁木齐 830054
3. 新疆医科大学第一附属医院临床研究院, 新疆维吾尔自治区 乌鲁木齐 830011
4. 新疆医科大学第二附属医院重症医学科, 新疆维吾尔自治区 乌鲁木齐 830000
5. 新疆医科大学第一附属医院综合内二科, 新疆维吾尔自治区 乌鲁木齐 830011
Correlation between transient receptor potential canonical channel with heart and kidney injure of rat model of obstructive sleep apnea hypopnea syndrome
WEN Wen1(),YAO Qiaoling2,CHEN Yulan1,*(),LI Zhiqiang3,SUN Xiaojing4,LI Yu5,ZHANG Junshi1,SIMAYIZhulipiya 1,XU Xinjuan1
1. Department of Hypertension, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
2. Department of Physiology, Preclinical Medicine Collage of Xinjiang Medical University, Urumqi 830054, China
3. Clinical Medical Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
4. Department of Critical Medicine, the Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, China
5. Second Department of General Internal Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China
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摘要：

目的: 探究经典瞬时受体电位通道C（TRPC）相关蛋白在阻塞性睡眠呼吸暂停低通气综合征（OSAHS）大鼠心脏和肾脏损害中的作用。方法: 18只SD雄性大鼠随机分为实验组和对照组，每组9只。实验组大鼠在间歇性低氧舱中，每天暴露于间歇性低氧环境8 h（10:00—18:00）。此后通过实时荧光定量PCR和蛋白质印迹法分别检测大鼠心脏和肾脏组织中TRPC mRNA和相关蛋白的表达。结果: 实验组心脏组织中TRPC3、TRPC4、TRPC5的mRNA表达较对照组升高（均P < 0.05），而肾脏组织TRPC1、TRPC3、TRPC4、TRPC5、TRPC6、TRPC7的mRNA表达在两组之间差异无统计学意义（均P>0.05）；实验组肾脏组织中TRPC4、TRPC5、TRPC6的mRNA表达低于心脏组织（均P < 0.05），对照组肾脏组织TRPC7的mRNA表达高于心脏组织（P < 0.05）。实验组心脏组织中的TRPC5蛋白表达较对照组升高（P < 0.05），而肾脏组织TRPC5、TRPC6、TRPC7相关蛋白的表达在两组之间差异无统计学意义（均P>0.05）。结论: TRPC5可能参与OSAHS心脏损害的病理生理过程，有望成为治疗OSAHS所致心脏损害的药物新靶点。
关键词： 瞬时受体电位通道/代谢;  蛋白质类;  睡眠呼吸暂停综合征/病理生理学;  低氧/病理学;  心脏/代谢;  肾/代谢;  疾病模型, 动物;  病例对照研究    
Abstract:

Objective: To investigate the expression of transient receptor potential canonical channels (TRPCs) in the heart and kidney of rat model of obstructive sleep apnea hypopnea syndrome (OSAHS). Methods: Eighteen male SD rats were randomly assigned to intermittent hypoxia (IH) group (n=9) and control group (n=9). In IH group, rats were placed in a chamber and exposed to intermittent hypoxia for 8h (10AM-6PM) daily. The expression of TRPC-related mRNA and protein in the heart and kidney tissue were detected by qRT-PCR and Western blotting, respectively. Results: The mRNA expressions of TRPC3/TRPC4/TRPC5 in heart tissues of IH group were increased significantly compared with the control group (all P>0.05); while there were no significant differences in the mRNA expressions of TRPC1/TRPC3/TRPC4/TRPC5/TRPC6/TRPC7 in kidney tissue between two groups (all P < 0.05). The mRNA expressions of TRPC4, TRPC5 and TRPC6 in kidney tissues of IH group were lower than that in heart tissues (all P < 0.05). The mRNA expression of TRPC7 in kidney tissues of control group was significantly higher than that in heart tissues (P < 0.05). The expression of TRPC5 protein in heart tissues of IH group was significantly higher than that in the control group (P < 0.05); while there was no significant differences in the expression of TRPC5/TRPC6/TRPC7 protein in kidney tissue between two groups (all P>0.05). Conclusion: The IH rat model shows that TRPC5 channel is likely to be involved in the OSAHS induced pathophysiological changes in the myocardium and may become a target to prevent OSAHS related cardiac damage.
Key words: Transient receptor potential channels/metabolism    Proteins    Sleep apnea syndromes/physiopathology    Hypoxia/pathology    Heart/metabolism    Kidney/metabolism    Disease models, animal    Case-control studies
收稿日期: 2019-09-17 出版日期: 2020-09-27
CLC:  R442.8  
基金资助: 国家自然科学基金(82060058)
通讯作者: 陈玉岚     E-mail: g.r315@163.com;sheliachen@sina.com
作者简介: 温雯(1991-), 女, 硕士研究生, 主要从事高血压及相关疾病研究; E-mail:g.r315@163.com; https://orcid.org/0000-0002-1966-7222
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引用本文:

温雯, 姚巧玲, 陈玉岚, 李志强, 孙晓靖, 李瑜, 张俊仕, 珠勒皮亚·司马义, 徐新娟. 瞬时受体电位通道C与阻塞性睡眠呼吸暂停低通气综合征大鼠心脏和肾脏损害的关系[J]. 浙江大学学报（医学版）, 2020, 49(4): 439-446.

WEN Wen, YAO Qiaoling, CHEN Yulan, LI Zhiqiang, SUN Xiaojing, LI Yu, ZHANG Junshi, SIMAYIZhulipiya , XU Xinjuan. Correlation between transient receptor potential canonical channel with heart and kidney injure of rat model of obstructive sleep apnea hypopnea syndrome. J Zhejiang Univ (Med Sci), 2020, 49(4): 439-446.

链接本文:

https://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.2020.04.16    或    https://www.zjujournals.com/med/CN/Y2020/V49/I4/439

引物名称 序列(5′→3′) 引物大小(bp)
TRPC：瞬时受体电位通道C.
TRPC1 正向：CTGCTTATCTTCATGTGCGGTC 138
反向：GAAGCTGTGGTAGGCTCTGT
TRPC3 正向：ACGCAGTACGGCAACATC 209
反向：CGCACATAGCCTTTGCTGAT
TRPC4 正向：AAGGATTAGCTTCACGGGGTG 198
反向：CCTCCTCCTGGGCGTGTTTC
TRPC5 正向：CCATACAGAGACCGCATCCC 283
反向：CCTTGCGGATGGCATAGAGT
TRPC6 正向：AAACAGACTGACTCACCGGC 238
反向：CGCCAACTGTAGGGCATTCT
TRPC7 正向：TTGGGGAGCAACACCTTCAA 97
反向：TGAACATGTAGGCAGGACCC
GAPDH 正向：CAGGGCTGCCTTCTCTTGTG 172
反向：GATGGTGATGGGTTTCCCGT
表 1  荧光定量PCR引物
图 1  实验组与对照组大鼠瞬时受体电位通道C(TRPC) mRNA水平比较
图 2  大鼠心脏组织与肾脏组织中瞬时受体电位通道C(TRPC) mRNA水平比较
图 3  瞬时受体电位通道C(TRPC)蛋白在大鼠心脏和肾脏组织中表达电泳图
组别 n TRPC5 TRPC6 TRPC7
心脏组织 肾脏组织 心脏组织 肾脏组织 心脏组织 肾脏组织
与对照组比较，*P＜0.05.
对照组 3 6.822±1.909 0.164±0.192 1.460±0.537 0.018±0.011 6.030±0.675 0.136±0.023
实验组 5 11.448±2.601* 0.135±0.112 1.530±0.767 0.014±0.005 5.028±1.836 0.117±0.018
表 2  实验组和对照组大鼠心脏和肾脏组织中瞬时受体电位通道C(TRPC)各蛋白表达水平比较
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