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浙江大学学报(医学版)  2019, Vol. 48 Issue (6): 625-630    DOI: 10.3785/j.issn.1008-9292.2019.12.06
原著     
二苯乙烯苷可抑制中波紫外线诱导的人皮肤成纤维细胞光老化
徐美娇1,2(),王一枫2,3,*()
1. 苏州市独墅湖医院(苏州大学附属独墅湖医院)皮肤科, 江苏 苏州 215000
2. 浙江中医药大学第二临床医学院, 浙江 杭州 310053
3. 浙江省立同德医院皮肤科, 浙江 杭州 310012
Stilbene glucoside inhibits ultraviolet radiation B-induced photoaging in human skin fibroblasts
XU Meijiao1,2(),WANG Yifeng2,3,*()
1. Department of Dermatology, Suzhou Dushuhu Public Hospital, Dushuhu Public Hospital Affiliated to Soochow University, Suzhou 215000, China
2. The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China
3. Department of Dermatology, Tongde Hospital of Zhejiang Province, Hangzhou 310012, China
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摘要:

目的: 研究二苯乙烯苷(TSG)对紫外线B(UVB)诱导的人皮肤成纤维细胞(HSF)应激性提早衰老的作用及可能的机制。方法: UVB小剂量、多次照射HSF,每次照射完毕后分别用0.02、0.10、0.50 mmol/L浓度的TSG处理。实验分为6组:空白对照组、模型对照组、UVB+0.02 mmol/L TSG组、UVB+0.10 mmol/L TSG组、UVB+0.50 mmol/L TSG组、TSG对照组。采用细胞计数法(CCK-8)检测细胞增殖活性;细胞化学染色法检测衰老相关β-半乳糖苷酶(SA-β-gal)表达;TBA法、WST-1法测定细胞丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性;ELISA法测定细胞上清液中基质金属蛋白酶-1(MMP-1)的含量变化。结果: 与空白对照组比较,模型对照组细胞增殖活性减弱(P < 0.05),SA-β-gal染色呈强阳性,细胞裂解液中SOD活性减弱、MDA含量增加,MMP-1浓度升高(P < 0.05或P < 0.01)。与模型对照组比较,UVB+各浓度TSG组细胞增殖活性改善(均P < 0.05),SA-β-gal染色阳性率下降,细胞裂解液中SOD活性增强、MDA含量减少,MMP-1浓度减小(P < 0.05或P < 0.01)。结论: TSG可以在一定程度上抑制UVB诱导的HSF应激性提早衰老,该作用可能与改善氧化应激、抑制MMP-1高表达有关。

关键词: 二苯乙烯类/药理学成纤维细胞/药物作用成纤维细胞/辐射效应皮肤/细胞学细胞衰老/辐射效应紫外线/副作用基质金属蛋白酶-1β半乳糖苷酶类    
Abstract:

Objective: To study the effects of tetrahydroxy stilbene-2-O-β-D-glucoside (TSG) on stress-induced premature senescence of human skin fibroblasts (HSF) exposed to ultraviolet radiation B (UVB) and its possible mechanism. Methods: HSFs were repeatedly exposed to UVB at a subcytotoxic level. TSG treatment (0.02, 0.10 and 0.50 mmol/L) was given immediately after each irradiation. The HSFs were divided into six groups:blank control group, model group, UVB+0.02 mmol/L TSG group, UVB+0.10 mmol/L TSG group, UVB+0.50 mmol/L TSG and TSG group (0.50 mmol/L). Cell counting kit-8 (CCK-8) was used to evaluate the proliferative activity of cells; senescence-associated-β-galactosidase (SA-β-gal) staining was performed to estimate the degree of premature senescence in cells; TBA method and WST-1 method were used to detect intracellular malondialdehyde (MDA) and superoxide dismutase (SOD) activities; and ELISA was applied to quantify the secretion level of matrix metalloproteinase1 (MMP-1) in cultured supernatant. Results: Compared with the blank control group, the proliferative activity and SOD level in the model group decreased (P < 0.05), while the percentage of SA-β-gal-positive cells, MDA and MMP-1 levels increased (P < 0.05 or P < 0.01). Compared with the model group, the proliferative activity and SOD level increased in UVB+TSG groups (all P < 0.05), and the percentage of SA-β-gal-positive cells, MDA and MMP-1 levels decreased (P < 0.05 or P < 0.01). Conclusion: TSG can inhibit UVB-induced premature senescence of HSF, which may be related to the inhibition of oxidative stress and high expression of MMP-1.

Key words: Stilbenes/pharmacology    Fibroblasts/drug effects    Fibroblasts/radiation effects    Skin/cytology    Cell aging/radiation effects    Ultraviolet rays/adverse effects    Matrix metalloproteinase 1    beta-Galactosidase
收稿日期: 2019-07-20 出版日期: 2020-01-19
:  R758.14  
基金资助: 浙江省公益性技术应用研究计划(2016C33204)
通讯作者: 王一枫     E-mail: ximigo307@126.com;wangyfen2006@163.com
作者简介: 徐美娇(1988—), 女, 硕士, 住院医师, 主要从事皮肤美容研究; E-mail:ximigo307@126.com; https://orcid.org/0000-0002-5116-4203
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引用本文:

徐美娇,王一枫. 二苯乙烯苷可抑制中波紫外线诱导的人皮肤成纤维细胞光老化[J]. 浙江大学学报(医学版), 2019, 48(6): 625-630.

XU Meijiao,WANG Yifeng. Stilbene glucoside inhibits ultraviolet radiation B-induced photoaging in human skin fibroblasts. J Zhejiang Univ (Med Sci), 2019, 48(6): 625-630.

链接本文:

http://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.2019.12.06        http://www.zjujournals.com/med/CN/Y2019/V48/I6/625

图 1  各组衰老相关β-半乳糖苷酶细胞化学染色图
图 2  各组SOD、MDA检测结果比较
图 3  各组衰老相关蛋白MMP-1浓度比较
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