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浙江大学学报(医学版)  2017, Vol. 46 Issue (4): 433-438    DOI: 10.3785/j.issn.1008-9292.2017.08.14
原著     
低盐饮食对犬类心脏组织基因表达的影响
任晓梅, 辛宝, 钱文文, 张荣强
陕西中医药大学公共卫生学院, 陕西 咸阳 712046
Effects of low salt diet on gene expression in dog's heart
REN Xiaomei, XIN Bao, QIAN Wenwen, ZHANG Rongqiang
School of Public Health, Shaanxi University of Chinese Medicine, Xianyang 712046, China
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摘要:

目的:探讨低盐饮食对哺乳动物心脏组织基因表达谱的影响,并分析差异表达基因可能涉及的分子生物学功能。方法:从基因芯片公共数据库(GEO)中获取低盐饮食心脏组织基因芯片数据(GSE17149),将数据导入Qlucore Omics Explorer 3.1、STRING 10.0、Genclip 2.0、GCBI等分析软件,筛选低盐饮食组与正常饮食组实验动物心脏组织差异表达基因,并分析差异表达基因编码蛋白质相互作用网络等,筛选两组之间的关键节点基因。结果:与正常饮食组比较,低盐饮食组犬心脏组织基因表达谱发生改变,在43 035个基因中共有1343个(3.12%)差异表达基因;差异表达基因蛋白质相互作用网络分析发现1个最明显的蛋白质互相作用网络,以NF-κB抑制因子-α(NFKBIA)蛋白为核心,主要发挥减弱巨噬细胞源性泡沫细胞分化、促进胆固醇排出和分解等生物学功能。结论:低盐饮食动物心脏组织基因转录谱发生明显改变,低盐饮食可能通过上调NFKBIA基因的表达来降低心血管疾病的风险。

关键词: 膳食限钠心血管疾病/预防和控制动脉粥样硬化/病理生理学基因表达转录遗传质谱分析法计算生物学细胞培养的    
Abstract:

Objective:To investigate the effect of low-salt diet on gene expression of canine cardiac tissue. Methods:Microarray data (GSE17149) of heart tissue from dogs fed with low-salt diet was obtained from the Gene Expression Omnibus (GEO). The data were analyzed by Qlucore Omics Explorer 3.1, STRING 10.0, Genclip 2.0 and GCBI. The protein-protein interactions (PPI) of the differentially expressed genes in low-salt and control groups were conducted to screen the key node genes between the two groups. Results:Compared with the control group, the gene expression profile of the dog heart tissue was changed in the low-salt diet group, and 1343 (3.12%) differentially expressed genes were found in 43 035 genes. The differentially expressed gene protein interaction analysis revealed a most obvious protein (NFKBIA) as the core, which might be related to inhibiting the differentiation of macrophage-derived foam cell and promoting cholesterol discharge and decomposition. Conclusion:Gene expression of heart tissue in dogs fed with low-salt diet is significantly changed and low-salt diet may reduce the risk of cardiovascular disease by up-regulating the expression of NFKBIA.

Key words: Diet, sodium-restricted    Cardiovascular diseases/prevention &    control    Gene expression    Atherosclerosis/physiopathology    Transcription, genetic    Mass spectrometry    Computational biology    Cells, cultured
收稿日期: 2017-04-19 出版日期: 2017-08-25
CLC:  R541.4  
通讯作者: 张荣强(1983-),男,博士研究生,讲师,主要从事慢性非传染性疾病的发病机制与预防控制研究;E-mail:zhangrqxianyang@163.com;http://orcid.org/0000-0003-3351-2128     E-mail: zhangrqxianyang@163.com
作者简介: 任晓梅(1985-),女,硕士,讲师,主要从事妇幼与常见慢性病营养研究;E-mail:250838718@qq.com;http://orcid.org/0000-0002-0032-4246
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引用本文:

任晓梅 等. 低盐饮食对犬类心脏组织基因表达的影响[J]. 浙江大学学报(医学版), 2017, 46(4): 433-438.

REN Xiaomei, XIN Bao, QIAN Wenwen, ZHANG Rongqiang. Effects of low salt diet on gene expression in dog's heart. Journal of ZheJiang University(Medical Science), 2017, 46(4): 433-438.

链接本文:

http://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.2017.08.14        http://www.zjujournals.com/med/CN/Y2017/V46/I4/433

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