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浙江大学学报（医学版）
浙江大学学报（医学版）  2017, Vol. 46 Issue (3): 290-294    DOI: 10.3785/j.issn.1008-9292.2017.06.11
出生缺陷预防专题     
不同氧浓度培养对体外受精-胚胎移植及子代出生缺陷的影响
李恩书, 叶晓群, 方力, 叶英辉
浙江大学医学院附属妇产科医院生殖内分泌科 生殖遗传教育部重点实验室, 浙江 杭州 310006
Effect of oxygen concentration on outcome of in-vitro fertilization-embryo transfer
LI Enshu, YE Xiaoqun, FANG Li, YE Yinghui
Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Key Laboratory of Reproductive Genetics, Ministry of Education, Hangzhou 310006, China
 全文: PDF(993 KB)  
摘要：

目的：比较低氧浓度与高氧浓度培养对体外受精-胚胎移植中胚胎发育、妊娠结局以及子代出生缺陷的影响。方法：根据胚胎体外培养的氧浓度，将2013至2015年在浙江大学医学院附属妇产科医院行体外受精-胚胎移植的患者随机分为低氧浓度组（n=2036，培养体系含氧气体积分数为5%）和高氧浓度组（n=4617，培养体系含氧气体积分数为20%）。比较两组间受精率、优质胚胎率、临床妊娠率、异位妊娠率、流产率以及子代出生缺陷率等结果指标。结果：低氧浓度组优质胚胎率高于高氧浓度组（P<0.05），但两组间受精率、临床妊娠率、异位妊娠率、流产率和子代出生缺陷的发生率差异均无统计学意义（均P>0.05）。结论：体外受精-胚胎移植采用低氧环境培养可能有助于提高胚胎发育潜能，但对妊娠结局和子代出生缺陷的影响不明显。
关键词： 氧;  受精;  体外;  胚胎移植;  新生儿筛查;  先天畸形;  胚胎发育;  妊娠结局    
Abstract:

Objective:To investigate the effect of low and high oxygen concentration on embryo development, pregnancy outcome and birth defects of in vitro fertilization-embryo transfer (IVF-ET). Methods:According to the oxygen concentration of in vitro culture environment, the IVF-ET performed in the Women's Hospital, Zhejiang University School of Medicine during 2013 and 2015 were divided into low oxygen concentration group (n=2036, 5% O2) and high oxygen concentration group (n=4617, 20% O2). The rate of fertilization, good quality embryo, clinical pregnancy, ectopic pregnancy, abortion and birth defect were compared between two groups. Results:The good quality embryo rate was significantly higher in the low oxygen concentration group (P<0.05). However, no significant differences were found between two groups in the rate of fertilization, clinical pregnancy, ectopic pregnancy, abortion and birth defect (all P>0.05). Conclusion:Low oxygen environment may improve the potential of embryonic development, but its impact on pregnancy outcome and birth defect is not significant.
Key words: Oxygen    Fertilization in vitro    Embryo transfer    Neonatal screening    Congenital abnormalities    Embryonic development    Pregnancy outcome
收稿日期: 2017-02-15 出版日期: 2017-06-25
CLC:  R715  
基金资助:

国家自然科学基金（81370761）；浙江省医药卫生科技计划（2014KYA252，201493615）
通讯作者: 叶英辉(1970-),女,博士,副研究员,硕士生导师,主要从事生殖遗传学研究;E-mail:yeyh1999@zju.edu.cn     E-mail: yeyh1999@zju.edu.cn
作者简介: 李恩书(1985-),女,硕士,实验师,主要从事辅助生殖技术研究;E-mail:lienshu@zju.edu.cn
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引用本文:

李恩书 等. 不同氧浓度培养对体外受精-胚胎移植及子代出生缺陷的影响[J]. 浙江大学学报（医学版）, 2017, 46(3): 290-294.

LI Enshu, YE Xiaoqun, FANG Li, YE Yinghui. Effect of oxygen concentration on outcome of in-vitro fertilization-embryo transfer. Journal of ZheJiang University(Medical Science), 2017, 46(3): 290-294.

链接本文:

http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2017.06.11    或    http://www.zjujournals.com/xueshu/med/CN/Y2017/V46/I3/290

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