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浙江大学学报（医学版）
浙江大学学报（医学版）  2016, Vol. 45 Issue (6): 587-591    DOI: 10.3785/j.issn.1008-9292.2016.11.05
骨组织代谢与再生专题     
丹参素对去势大鼠骨质量的影响
屈涛, 甄平, 杨成伟, 蓝旭, 张涛, 刘华, 王世勇
兰州军区兰州总医院全军骨科中心脊柱外科, 甘肃 兰州 730050
Effects of Danshensu on bone formation in ovariectomized rats
QU Tao, ZHENG Ping, YANG Chengwei, LAN Xu, ZHANG Tao, LIU Hua, WANG Shiyong
Spine Surgery of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China
 全文: PDF(1070 KB)  
摘要：

研究口服丹参素能否促进去势大鼠骨形成或减少骨丢失，从而提高骨质量。方法：30只雌性SD大鼠随机分为三组，即空白对照组、模型对照组和丹参素组，每组10只。模型对照组和丹参素组行双侧卵巢切除术，其中，丹参素组采用丹参素灌胃（12.5 mg·kg-1·d-1）。90 d后处死所有大鼠，采用双能X线骨密度仪检测大鼠全身骨密度、股骨和腰椎骨骨密度，AG-IS型电子万能试验机检测股骨生物力学性能，ELISA法检测血清骨钙素和骨碱性磷酸酶（BALP）水平，HE染色后统计股骨近心干骺端成骨细胞数量。结果：与空白对照组比较，模型对照组骨密度、股骨生物力学指标、血清骨钙素和BALP水平均降低，骨组织中成骨细胞数量减少；而丹参素组全身骨密度、股骨和腰椎骨骨密度、股骨三点弯曲试验弹性模量、最大载荷、屈服强度、断裂点载荷、血清骨钙素和BALP水平及成骨细胞数量均高于模型对照组（P<0.05或P<0.01）。结论：丹参素可提高大鼠骨密度，增强大鼠生物力学性能，促进血清骨形成相关因子的表达以及增加成骨细胞数量，从而提高骨质量。
关键词： 丹参素/治疗应用;  骨质疏松/药物疗法;  骨密度;  生物力学;  碱性磷酸酶;  骨钙素;  大 鼠;  Sprague-Dawley;  模型;  动物;  随机对照试验    
Abstract:

Objective: To investigate the effects of Danshensu on bone formation in ovariectomized rats.Methods: Thirty female SD rats were randomly divided into three groups with 10 rats in each:blank control group, model control group and Danshensu group. The osteoporosis model was induced by bilateral ovariectomy and rats in Danshensu group were fed with Danshensu 12.5 mg·kg-1·d-1 by gavage after ostroporosis model induced. All animals were sacrificed after 90 days. The bone mineral density (BMD) of the whole body, femur and lumbar vertebra was measured by dual energy X-ray absorptiometry. The biomechanical properties of femur were measured by AG-IS mechanical universal testing machine. Serum osteocalcin and bone alkaline phosphates (BALP) levels were measured by ELISA. The number of osteoblasts of proximal femoral metaphysis was counted with light microscopy after HE staining. Results: Compared with blank control group, BMD, biomechanical properties of femur, serum osteocalcin and BALP levels and the number of osteoblasts were decreased in model control group (P<0.05 or P<0.01). While compared with model control group, BMDs of the whole body, femur and lumbar vertebra, the elastic modulus, maximum load, yield strength, breaking point load of femur, the serum levels of osteocalcin and BALP, and the number of osteoblasts were significantly improved in Danshensu group (P<0.05 or P<0.01). Conclusion: Danshensu can improve bone quality by increasing bone density, improving biomechanical properties, promoting the expression of osteogenesis-related factors, and increasing the number of osteoblasts.
Key words: Danshensu/therapeutic use    Osteoporosis/drug therapy    Bone density    Biomechanics    Alkaline phosphatase    Osteocalcin    Rats, Sprague-Dawley    Models, animal    Randomized controlled trial
收稿日期: 2016-10-10
CLC:  R285.5  
基金资助:

国家自然科学基金（81601905）
通讯作者: 王世勇(1967-),男,硕士,副主任医师,主要从事脊柱外科工作;E-mail:wsyonggansu@126.com;http://orcid.org/0000-0001-7665-5933     E-mail: wsyonggansu@126.com
作者简介: 屈涛(1980-),男,学士,主治医师,主要从事脊柱外科工作;E-mail:QuTao1980@126.com;http://orcid.org/0000-0001-7665-5933
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引用本文:

屈涛 等. 丹参素对去势大鼠骨质量的影响[J]. 浙江大学学报（医学版）, 2016, 45(6): 587-591.

QU Tao, ZHENG Ping, YANG Chengwei, LAN Xu, ZHANG Tao, LIU Hua, WANG Shiyong. Effects of Danshensu on bone formation in ovariectomized rats. Journal of ZheJiang University(Medical Science), 2016, 45(6): 587-591.

链接本文:

http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2016.11.05    或    http://www.zjujournals.com/xueshu/med/CN/Y2016/V45/I6/587

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