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浙江大学学报（医学版）
浙江大学学报（医学版）  2016, Vol. 45 Issue (6): 581-586    DOI: 10.3785/j.issn.1008-9292.2016.11.04
骨组织代谢与再生专题     
淫羊藿总黄酮胶囊对生长期大鼠骨密度和骨形态计量学的影响
高玉海, 杨芳芳, 葸慧荣, 李文苑, 甄平, 陈克明
兰州军区兰州总医院全军骨科中心骨科研究所, 甘肃 兰州 730050
Effects of icariin total flavonoids capsule on bone mineral density and bone histomorphometry in growing rats
GAO Yuhai, YANG Fangfang, XI Huirong, LI Wenyuan, ZHEN Ping, CHEN Keming
Institute of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China
 全文: PDF(1130 KB)  
摘要：

目的：研究淫羊藿总黄酮胶囊（ITFC）对生长期大鼠峰值骨密度和骨形态计量学的影响，探索其抗骨质疏松的作用机制。方法：30只雌性SD大鼠按体质量随机分为正常对照组、ITFC-1组及ITFC-2组。其中，ITFC-1组和ITFC-2组分别以50 mg·kg-1·d-1 ITFC和100 mg·kg-1·d-1ITFC灌胃，正常对照组灌服等体积蒸馏水。在灌胃后4、8、12周测定全身骨密度。12周后处死大鼠检测右侧股骨和腰椎骨骨密度；ELISA法检测血清抗酒石酸酸性磷酸酶5b（TRACP 5b）和骨碱性磷酸酶（BALP）水平；右侧胫骨进行骨形态计量学分析。结果：与正常对照组比较，ITFC-1组和ITFC-2组大鼠体质量增加减少，但差异无统计学意义（均P>0.05）。4周和8周时，ITFC-1组和ITFC-2组全身骨密度均有所提高，但与正常对照组差异无统计学意义（均P>0.05）。12周时，ITFC-1组和ITFC-2组大鼠全身骨密度、离体骨密度及血清BALP和骨小梁面积百分比均高于正常对照组（均P<0.05），骨小梁分离度均低于正常对照组（均P<0.05）；ITFC-2组大鼠骨小梁宽度和数量均高于正常对照组，血清TRACP 5b低于正常对照组（均P<0.05）；ITFC-2组大鼠离体骨密度、血清BALP、骨小梁数量和面积百分比均高于ITFC-1组，骨小梁分离度和血清TRACP 5b均低于ITFC-1组（均P<0.05）。结论：ITFC有可能通过提高骨密度、增加骨形成、降低骨吸收及改善骨组织微结构来预防老年骨质疏松的发生。
关键词： 黄酮类/投药和剂量;  淫羊藿甙/治疗应用;  骨质疏松/中药疗法;  骨和骨组织/解剖学和组织学;  疾病模型,动物;  骨密度    
Abstract:

Objective: To investigate the effect of icariin total flavonoids capsules (ITFC) on bone mineral density (BMD) and bone histomorphometry in growing rats and its anti-osteoporosis mechanism. Methods: Thirty female SD rats were randomly divided into 3 groups:normal control group, ITFC-1 group and ITFC-2 group. Rats in ITFC-1 group and ITFC-2 group were fed with 50 mg·kg-1·d-1 or 100 mg·kg-1·d-1 ITFC, respectively, and those in normal control group were fed with equal volume of distilled water. The whole body BMD was measured after 4, 8 and 12 weeks, and BMDs of the right femur and lumbar vertebrae were measured after 12 weeks. The serum levels of tartaric acid phosphatase 5b (TRACP 5b) and bone alkaline phosphatase (BALP) were measured by ELISA. Bone morphometry was performed on the right tibia. Results: There were no significant differences in the body weight increase between normal control group and two ITFC groups (all P>0.05). There were also no significant differences in whole body BMDs after 4 and 8 weeks between normal control group and ITFC groups (all P>0.05). After 12 weeks, the whole body BMD, BMD of bone in vitro, serum BALP level and trabecular area in ITFC-1 group and ITFC-2 group were significantly higher, trabecular separation was significantly lower than that in normal control group (all P<0.05); and the trabecular width and the number in ITFC-2 group were also significantly higher, and serum TRACP 5b level was significantly lower than that in normal control group (all P<0.05). The BMD of bone in vitro, serum BALP level, trabecular number and area in ITFC-2 group were significantly higher, and serum TRACP 5b level was significantly lower than that in ITFC-1 group (all P<0.05). Conclusion: ITFC can prevent osteoporosis by increasing bone density and bone formation, decreasing bone resorption and improving microstructure of bone.
Key words: Flavones/administration &dosage    Icariin/therapeutic use    Osteoporosis/traditional Chinese drug therapy    Bone and bones/anatomy &histology    Disease models, animal    Bone density
收稿日期: 2016-08-08
CLC:  R68  
基金资助:

国家自然科学基金（81270963，81471090）；甘肃省自然科学基金（1506RJZA307）；甘肃省空间辐射生物学重点实验室开放基金（2016GKL002）
通讯作者: 陈克明(1968-),男,博士,教授,博士生导师,主要从事骨质疏松症的发病机制和防治研究;E-mail:chenkm@lut.cn;http://orcid.org/0000-0002-9749-8356     E-mail: chenkm@lut.cn
作者简介: 高玉海(1988-),男,硕士,技师,主要从事骨代谢动物实验研究;E-mail:gyh1389@126.com;http://orcid.org/0000-0001-7845-3796
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引用本文:

高玉海 等. 淫羊藿总黄酮胶囊对生长期大鼠骨密度和骨形态计量学的影响[J]. 浙江大学学报（医学版）, 2016, 45(6): 581-586.

GAO Yuhai, YANG Fangfang, XI Huirong, LI Wenyuan, ZHEN Ping, CHEN Keming. Effects of icariin total flavonoids capsule on bone mineral density and bone histomorphometry in growing rats. Journal of ZheJiang University(Medical Science), 2016, 45(6): 581-586.

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http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2016.11.04    或    http://www.zjujournals.com/xueshu/med/CN/Y2016/V45/I6/581

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