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浙江大学学报（医学版）
浙江大学学报（医学版）  2016, Vol. 45 Issue (6): 568-574    DOI: 10.3785/j.issn.1008-9292.2016.11.02
骨组织代谢与再生专题     
低频脉冲电磁场促进成骨细胞分化的基因调节和非基因调节探究
方清清1, 李志忠2, 周建1, 石文贵1, 闫娟丽1, 陈克明1
1. 兰州军区兰州总医院全军骨科中心骨科研究所, 甘肃 兰州 730050;
2. 兰州理工大学生命科学与工程学院, 甘肃 兰州 730050
Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation
FANG Qingqing1, LI Zhizhong2, ZHOU Jian1, SHI Wengui1, YAN Juanli1, CHEN Keming1
1. Institute of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China;
2. College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China
 全文: PDF(1110 KB)  
摘要：

目的：探究50 Hz 0.6 mT脉冲电磁场（PEMF）促进体外培养大鼠颅骨成骨细胞（ROB）分化成熟是否存在基因调节和非基因调节。方法：体外培养ROB，传代融合后经50 Hz 0.6 mT PEMF处理1.5 h，于0、3、6、9、12 h检测碱性磷酸酶（ALP）的活性及其基因表达，以及骨形成相关因子Runx2、OSX的基因和蛋白表达。结果：50 Hz 0.6 mT PEMF处理后3 h，ROB中ALP的活性高于对照组（P<0.01），而其基因表达在处理后6 h才高于对照组。Runx2的基因表达在处理后立即升高，持续至处理后6 h回归对照组水平，之后再次升高，其蛋白表达与之相对应但略有滞后。OSX的基因表达亦在处理后立即升高，持续至处理后6 h回归对照组水平，至12 h时显著降低，其蛋白表达与之相对应但略有滞后。结论：50 Hz 0.6 mT PEMF促进体外培养ROB分化成熟存在对Runx2和OSX的基因调节以及对ALP活性的非基因调节。
关键词： 成骨细胞/辐射效应;  颅骨;  电磁场;  动物;  新生;  细胞;  培养的;  细胞分化;  基因;  调节;  大鼠;  Wistar    
Abstract:

Objective: To study the genic and non-genic regulation of 50 Hz 0.6 mT pulsed electromagnenic fields (PEMF) on rat calvarial osteoblasts (ROB) differentiation. Methods: ROBs were achieved by enzyme digestion, and treated with 50 Hz 0.6 mT PEMFs for 1.5 hours after subculture. The alkaline phosphatase (ALP) activity, mRNA transcription of ALP, Runx2 and OSX and protein expression of Runx2 and OSX were detected at 0, 3, 6, 9 and 12 hours after PEMF treatment. Results: The ALP activity at 3 hours after treatment was significantly higher than that in the control(P<0.01), while the mRNA transcription of ALP began to increase at 6 hours after treatment. The mRNA transcription of Runx2 increased immediately after treatment and regressed at 6 hours, then increased again. The protein expression of it corresponded but with a little lag. The mRNA transcription of OSX also raised instantly after treatment, then returned to the level of control at 6 hours, and lower than control at 12 hours significantly. The protein expression of it also corresponded but with a bit delay. Conclusions: There are genic regulation for the protein expression of Runx2 and OSX, and non-genic regulation for the ALP activity on the process of 50 Hz 0.6 mT PEMFs prompts ROBs differentiation.
Key words: Osteoblasts/radiation effects    Skull    Electromagnetic fields    Animals, newborn    Cells, cultured    Cell differentiation    Genes, regulator    Rats, Wistar
收稿日期: 2016-09-30
CLC:  Q683  
基金资助:

国家自然科学基金（81270963，81471090）
通讯作者: 陈克明(1968-),男,博士,教授,博士生导师,主要从事骨质疏松症的发病机制和防治研究,E-mail:chenkm@lut.cn;http://orcid.org/0000-0002-9749-8356     E-mail: chenkm@lut.cn
作者简介: 方清清(1988-),女,硕士研究生,主要从事低频电磁场防治骨质疏松症的研究;E-mail:qingqinggslz@126.com;http://orcid.org/0000-0003-0996-2639
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引用本文:

方清清 等. 低频脉冲电磁场促进成骨细胞分化的基因调节和非基因调节探究[J]. 浙江大学学报（医学版）, 2016, 45(6): 568-574.

FANG Qingqing, LI Zhizhong, ZHOU Jian, SHI Wengui, YAN Juanli, CHEN Keming. Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation. Journal of ZheJiang University(Medical Science), 2016, 45(6): 568-574.

链接本文:

http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2016.11.02    或    http://www.zjujournals.com/xueshu/med/CN/Y2016/V45/I6/568

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