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浙江大学学报（医学版）
浙江大学学报（医学版）  2016, Vol. 45 Issue (4): 335-341    DOI: 10.3785/j.issn.1008-9292.2016.07.02
癌分子医学专题     
肿瘤相关成纤维细胞CD10表达在结直肠腺瘤癌变和复发中的意义
郑江江1,2, 朱音1,2, 李昌水2, 李银亚2, 聂倩倩1, 朱紫菱1, 邓红1
1. 浙江大学医学院病理与病理生理学系, 浙江 杭州 310058;
2. 宁波市鄞州第二医院病理科, 浙江 宁波 315100
Expression of CD10 in tumor-associated fibroblast of cancerized or recurrent colorectal adenomas
ZHENG Jiangjiang1,2, ZHU Yin1,2, LI Changshui2, LI Yinya2, NIE Qianqian1, ZHU Ziling1, DENG Hong1
1. Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, Hangzhou 310058, China;
2. Department of Pathology, Yinzhou Second Hospital of Ningbo City, Ningbo 315100, China
全文: PDF(1033 KB)  
摘要: 

目的：探讨肿瘤相关成纤维细胞（TAF）CD10在结直肠腺瘤癌变和复发中的作用，评价TAF CD10表达在诊断腺瘤癌变中的应用价值。方法：分别收集结直肠低级别腺瘤、高级别腺瘤和结直肠腺癌组织各50例，取配对结直肠腺癌标本远端切缘为正常对照，采用免疫组织化学Envision法测定TAF CD10和肿瘤细胞p53、Ki-67、β-catenin及CyclinD1的表达，分析TAF表达CD10与肿瘤细胞表达p53、Ki-67、β-catenin和CyclinD1的相关性。同时收集低级别腺瘤100例，分复发组（43例）和非复发组（57例）两组，其中复发组中复发为结直肠腺癌16例和复发为腺瘤27例，比较CD10在复发组与非复发组间差异及复发为结直肠腺癌与腺瘤间差异。结果：正常黏膜不存在TAF，其CD10在结直肠低级别腺瘤、高级别腺瘤和腺癌中的阳性率分别为22%、50%和78%，呈逐渐升高趋势（均P<0.05）；Ki-67、β-catenin在低级别腺瘤、高级别腺瘤、腺癌中瘤细胞的表达逐渐升高（均P<0.01）；CyclinD1在高级别腺瘤中表达高于腺癌和低级别腺瘤（均P>0.05）；p53在结直肠腺癌和高级别腺瘤中表达高于低级别腺瘤（均P<0.01）；CD10的表达水平与肿瘤细胞p53、Ki-67、β-catenin核阳性表达均呈正相关（r=0.264、0.307、0.320，均P<0.01），而与CyclinD1、β-catenin膜阳性表达无明显相关（r=0.012、-0.073，均P>0.05）。CD10在低级别腺瘤复发组表达高于非复发组（P<0.05），在复发为结直肠腺癌组织的表达又高于复发为腺瘤者（P<0.05）。结论：TAF CD10可能在腺瘤癌变中起重要作用；TAF CD10高表达的腺瘤容易复发且癌变；TAF CD10联合肿瘤细胞p53、Ki-67、β-catenin检测有助于诊断结直肠腺瘤癌变和预测腺瘤复发。
关键词 ： 成纤维细胞,  白细胞分化抗原10,  结直肠肿瘤/病理学,  结直肠肿瘤/诊断,  腺癌/病理学,  腺癌/诊断,  肿瘤复发,局部,  免疫组织化学    
Abstract：

Objective: To investigate the expression of CD10 in tumor-associated fibroblasts (TAF) in colorectal adenomas and its relation to cancerization and recurrence of adenoma. Methods: Tissue samples of low-grade adenoma (n=50), high-grade adenoma (n=50) and colorectal adenocarcinoma (n=50) were collected, and tissue samples at the distal margin of corresponding colorectal lesions were taken as controls. The expression of CD10 in the stromal TAFs, and the expressions of β-catenin, Ki-67, p53 and CyclinD1 in tumor cells were detected by immunohistochemistry (Envision). The correlation of CD10 expression in stromal TAFs with the expressions of β-catenin, Ki-67, p53 and CyclinD1 in tumor cells was analyzed by Spearmen. One hundred samples of low-grade colorectal adenoma were collected, including 57 non-recurrent cases and 43 recurrent cases (16 cases of recurrent adenoma and 27 cases of recurrent adenocarcinoma); the expression of stromal TAF CD10 were determined and compared among groups. Results: There was no TAF in normal colorectal mucosa. The expression rates of TAF CD10 in low-grade adenoma, high-grade adenoma and colorectal adenocarcinoma were 22%, 50% and 78%, respectively (all P<0.05). The expression of Ki-67 and β-catenin in low-grade adenoma, high-grade adenoma, colorectal adenocarcinoma was on a rising trend (all P<0.01). The expression of CyclinD1 in high-grade adenoma was higher than that in colorectal adenocarcinoma and low-grade adenoma (all P>0.05). The expression of p53 in colorectal adenocarcinoma and high-grade adenoma was higher than that in low grade adenoma (all P<0.01). The expression of TAF CD10 was correlated with the expression of p53, Ki-67 and β-catenin-nucleus(r=0.264、0.307、0.320, all P<0.01),but not correlated with CyclinD1 and β-catenin-membrane (r=0.012、-0.073, all P>0.05). The TAF CD10 level was significantly higher in low-grade adenoma with recurrence than that in those without recurrence (P<0.05).The expression of CD10 in recurrent colorectal adenocarcinoma was higher than that in recurrent adenoma (P<0.05). Conclusion: The expression of TAF CD10 is increased gradually in the process of adenoma-cancer, indicating that it may play an important role in the canceration of adenoma. Adenomas with high expression of CD10 TAF are likely to be recurrent and cancerized, and detection of TAF CD10 combined with p53, Ki-67 and β-catenin may be of value in predicting canceration or recurrence of colorectal adenoma.
Key words： Fibroblasts    Cluster of differentiation 10    Colorectal neoplasms/pathology    Colorectal neoplasms/diagnosis    Adenocarcinoma/pathology    Adenocarcinoma/diagnosis    Neoplasm recurrence, local    Immunohistochemistry
收稿日期: 2016-03-15
CLC:  R735.3  
基金资助:

浙江省自然科学基金（LY12H16027）；国家自然科学基金（30870971）
通讯作者: 邓红(1964-),女,博士,副教授,硕士生导师,主要从事肿瘤分子病理学研究;E-mail:hongdeng@zju.edu.cn;http://orcid.org/0000-0002-6815-9144     E-mail: hongdeng@zju.edu.cn
作者简介: 郑江江(1984-),女,硕士研究生,主治医师,主要从事消化系统和泌尿生殖系统临床病理诊断工作;E-mail:zhengjj1984@sina.cn;http://orcid.org/0000-0001-7973-0556.朱音(1984-),女,硕士研究生,主治医师,主要从事消化系统和泌尿生殖系统临床病理诊断工作;E-mail:zhuyinsh@163.com;http://orcid.org/0000-0002-5805-9545
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郑江江 等. 肿瘤相关成纤维细胞CD10表达在结直肠腺瘤癌变和复发中的意义[J]. 浙江大学学报（医学版）, 2016, 45(4): 335-341.
ZHENG Jiangjiang, ZHU Yin, LI Changshui, LI Yinya, NIE Qianqian, ZHU Ziling, DENG Hong. Expression of CD10 in tumor-associated fibroblast of cancerized or recurrent colorectal adenomas. Journal of ZheJiang University(Medical Science), 2016, 45(4): 335-341.

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http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2016.07.02      或      http://www.zjujournals.com/xueshu/med/CN/Y2016/V45/I4/335

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