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浙江大学学报（医学版）
浙江大学学报（医学版）  2016, Vol. 45 Issue (3): 275-280    DOI: 10.3785/j.issn.1008-9292.2016.05.09
原著     
新生小鼠皮层神经元动作电位发放模式研究
刘芙蓉, 李胜天
上海交通大学Bio-X研究院, 上海 200240
Patterns of action potential firing in cortical neurons of neonatal mice and their electrophysiological property
LIU Furong, LI Shengtian
Bio-X Institute of Shanghai Jiaotong University, Shanghai 200240, China
全文: PDF(1051 KB)  
摘要: 

目的：探究新生小鼠皮层神经元的动作电位发放模式及不同发放模式的电学特性。方法：使用全细胞膜片钳技术，结合电压钳和电流钳模式，采用出生3 d小鼠皮层神经元观察不同神经元的主动和被动电学特性。结果：给予神经元去极化电流时记录到了三种动作电位发放模式：强直发放、阶段性发放和单个发放。比较三种动作电位发放模式的神经元的电学特性发现：①三种动作电位发放模式在神经元的电压依赖性内向和外向电流上存在差异；②三种动作电位发放模式的神经元在去极化电流阈值上也存在差异；③三种动作电位神经元在第一个动作电位的上升时间、宽度、幅值和阈值方面不同；④不同动作电位发放模式的神经元在被动电学特性上没有差异。结论：新生小鼠皮层神经元存在不同的动作电位发放模式，其电压依赖性内向电流和外向电流、动作电位形态、去极化电流阈值亦存在差异。
关键词 ： 小鼠,  动物,新生,  电生理学,  神经元/生理学,  膜片钳术,  动作电位,  细胞,培养的    
Abstract：

Objective: To investigate patterns of action potential firing in cortical heurons of neonatal mice and their electrophysiological properties. Methods: The passive and active membrane properties of cortical neurons from 3-d neonatal mice were observed by whole-cell patch clamp with different voltage and current mode. Results: Three patterns of action potential firing were identified in response to depolarized current injection. The effects of action potential firing patterns on voltage-dependent inward and outward current were found. Neurons with three different firing patterns had different thresholds of depolarized current. In the morphology analysis of action potential, the three type neurons were different in rise time, duration, amplitude and threshold of the first action potential evoked by 80 pA current injection. The passive properties were similar in three patterns of action potential firing. Conclusion: These results indicate that newborn cortical neurons exhibit different patterns of action potential firing with different action potential parameters such as shape and threshold.
Key words： Mice    Animals, newborn    Electrophysiology    Neurons/physiology    Patch-clamp techniques    Action otentials    Cells, cultured
收稿日期: 2015-12-03
CLC:  Q424  
基金资助:

国家自然科学基金（31271198）；上海市自然科学基金（13DJ1400303）
通讯作者: 李胜天(1967-),男,博士,副教授,主要从事突触可塑性和癫痫发生机制研究;E-mail:lstian@sjtu.edu.cn;http://orcid.org/0000-0002-2836-3802     E-mail: lstian@sjtu.edu.cn
作者简介: 刘芙蓉(1992-),女,硕士研究生,主要从事神经元电生理特性研究;E-mail:amandalfr@sjtu.edu.cn;http://orcid.org/0000-0001-6189-6259
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引用本文:

刘芙蓉 等. 新生小鼠皮层神经元动作电位发放模式研究[J]. 浙江大学学报（医学版）, 2016, 45(3): 275-280.
LIU Furong, LI Shengtian. Patterns of action potential firing in cortical neurons of neonatal mice and their electrophysiological property. Journal of ZheJiang University(Medical Science), 2016, 45(3): 275-280.

链接本文:

http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2016.05.09      或      http://www.zjujournals.com/xueshu/med/CN/Y2016/V45/I3/275

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