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浙江大学学报（医学版）
浙江大学学报（医学版）  2016, Vol. 45 Issue (2): 126-131    DOI: 10.3785/j.issn.1008-9292.2016.03.04
运动系统再生医学专题     
基于三维打印的钙化层重建生物活性支架制备及其性能研究
余新宁1, 方晶华1, 骆建洋1, 杨贤燕2, 何冬霜2, 苟中入2, 戴雪松1
1. 浙江大学医学院附属第二医院骨科 浙江大学骨科研究所, 浙江 杭州 310009;
2. 浙江加州国际纳米技术研究院, 浙江 杭州 310058
Fabrication of bioactive tissue engineering scaffold for reconstructing calcified cartilage layer based on three-dimension printing technique
YU Xinning1, FANG Jinghua1, LUO Jianyang1, YANG Xianyan2, HE Dongshuang2, GOU Zhongru2, DAI Xuesong1
1. Department of Orthopedic Surgery, Orthopedics Research Institute of Zhejiang University, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China;
2. Zhejiang-California International Nanosystems Institute, Hangzhou 310058, China
全文: PDF(1235 KB)  
摘要: 

目的: 初步构建基于软骨钙化层损伤重建的有机—无机复合组织工程支架,探究掺镁硅灰石含量与支架抗压性能之间的关系。方法: 利用质量分数分别为1%、3%、5%的高生物活性钙镁硅酸盐超细颗粒复合Ⅰ型胶原—透明质酸钠进行三维打印,经海藻酸钠—氯化钙气雾交联成型,电镜下观察表面孔隙、孔径、无机相分布,万能材料试验机测试抗压性能,并计算支架孔隙率。结果: 支架表面平均孔径(212.3±34.2)μm,平均孔隙率(48.3±5.9)%,不同质量分数的高生物活性钙镁硅酸盐超细颗粒复合Ⅰ型胶原—透明质酸钠支架压缩模量差异无统计学意义(P>0.05),平均压缩模量(7.2±1.2)MPa,介于软骨和软骨下骨之间。结论: 利用三维打印技术成功构建出多孔钙化层仿生重建支架,可为今后研制多层次复合支架治疗骨—软骨损伤奠定基础。
关键词 ： 软骨疾病/治疗,  硅,  钙化合物,  透明质酸,  胶原Ⅰ型,  计算机辅助设计,  成像,三维,  海藻酸钠,  支架    
Abstract：

Objective: To fabricate organic-inorganic composite tissue engineering scaffolds for reconstructing calcified cartilage layer based on three-dimensional (3D) printing technique. Methods: The scaffolds were developed by 3D-printing technique with highly bioactive calcium-magnesium silicate ultrafine particles of 1%, 3% and 5% of mass fraction, in which the organic phases were composed of type I collagen and sodium hyaluronate. The 3D-printed scaffolds were then crosslinked and solidified by alginate and CaCl2 aerosol. The pore size and distribution of inorganic phase were observed with scanning electron microscope (SEM); the mechanical properties were tested with universal material testing machine, and the porosity of scaffolds was also measured. Results: Pore size was approximately (212.3±34.2) μm with a porosity of (48.3±5.9)%, the compressive modulus of the scaffolds was (7.2±1.2) MPa, which was irrelevant to the percentage changes of calcium-magnesium silicate, the compressive modulus was between that of cartilage and subchondral bone. Conclusion: The porous scaffolds for calcified cartilage layer have been successfully fabricated, which would be used for multi-layered composite scaffolds in osteochondral injury.
Key words： Cartilage diseases/therapy    Silicon    Calcium compounds    Hyaluronic acid    Collagen type I    Computer-aided design    Imaging, three-dimensional    Sodiwm alginate    Scaffolds
收稿日期: 2015-10-12     
CLC:  R68  
基金资助:

国家自然科学基金(81472063);浙江省自然科学基金(LZ14E020001)
通讯作者: 戴雪松(1970-),男,博士,从事骨科和运动医学研究;E-mail:daixshz@163.com;苟中入(1975-),男,博士,从事生物医用纳米材料、仿生材料及相关细胞生物学和组织学研究;E-mail:zhrgou@zju.edu.cn     E-mail: daixshz@163.com;zhrgou@zju.edu.cn
作者简介: 余新宁(1991-),男,硕士研究生,从事骨科和运动医学研究;E-mail:3100104989@zju.edu.cn
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引用本文:

余新宁 等. 基于三维打印的钙化层重建生物活性支架制备及其性能研究[J]. 浙江大学学报（医学版）, 2016, 45(2): 126-131.
YU Xinning, FANG Jinghua, LUO Jianyang, YANG Xianyan, HE Dongshuang, GOU Zhongru, DAI Xuesong. Fabrication of bioactive tissue engineering scaffold for reconstructing calcified cartilage layer based on three-dimension printing technique. Journal of ZheJiang University(Medical Science), 2016, 45(2): 126-131.

链接本文:

http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2016.03.04      或      http://www.zjujournals.com/xueshu/med/CN/Y2016/V45/I2/126

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