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浙江大学学报（医学版）
浙江大学学报（医学版）  2016, Vol. 45 Issue (2): 105-111    DOI: 10.3785/j.issn.1008-9292.2016.03.01
指南与实践     
人胚胎干细胞向肌腱细胞分化方法的专家共识
陈晓1, 邹晓晖2, 俞光岩3, 傅歆4, 曹彤5, 肖殷6, 欧阳宏伟1
1. 浙江大学医学院干细胞与再生医学系 浙江大学李达三·叶耀珍干细胞与再生医学研究中心 浙江省组织工程与再生医学技术重点实验室, 浙江 杭州 310058;
2. 浙江大学医学院附属第一医院中心实验室, 浙江 杭州 310003;
3. 北京大学口腔医学院口腔颌面外科, 北京 100081;
4. 中国医学科学院整形外科医院研究中心, 北京 100144;
5. 新加坡国立大学口腔医学院口腔科学系干细胞实验室, 新加坡 119083;
6. 澳大利亚昆士兰科技大学健康与生物医学研究所, 澳大利亚 布里斯班QLD 4059
Expert consensus on induction of human embryonic stem cells into tenocytes
CHEN Xiao1, ZOU Xiaohui2, YU Guangyan3, FU Xin4, CAO Tong5, XIAO Yin6, OUYANG Hongwei1
1. Center of Stem Cell and Tissue Engineering, Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Key Laboratory of Stem Cell of Zhejiang Province, Zhejiang University School of Medicine, Hangzhou 310058, China;
2. Central Laboratory, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China;
3. Department of Oral and Maxillofacial Surgery, Peking University School of Stomatology, Beijing 100081, China;
4. Research Center of Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100144, China;
5. Faculty of Dentistry Research Laboratories, National University of Singapore, Singapore 119083, Singapore;
6. Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove QLD 4059, Australia
全文: PDF(1336 KB)  
摘要: 

胚胎干细胞具有无限增殖能力,可能为组织工程提供肌腱细胞或肌腱祖细胞的来源。为规范人胚胎干细胞来源的肌腱细胞的分化方法,提高人肌腱细胞的纯度,保证分化方法的可重复性,使其更系统、规范和有效地应用于临床和生物安全性评价体系中,科技部国际合作专项有关专家制定了《人胚胎干细胞向肌腱细胞分化方法共识》。共识建议通过两步法将人胚胎干细胞诱导为肌腱细胞:首先利用不同材料表面将人胚胎干细胞诱导为成体间充质干细胞,然后采用高密度种植形成无支架组织工程肌腱,并利用静态或动态力学刺激在体内外环境下将成体间充质干细胞诱导成肌腱细胞。由此建立的体外组织工程肌腱可作为检测评价模型,对小分子化合物、医用材料和药物进行肌腱相关的毒理学系统分析和安全性评价。
关键词 ： 胚胎干细胞,  腱/细胞学,  间质干细胞,  组织工程,  细胞分化,  细胞培养技术    
Abstract：

Embryonic stem cells have unlimited proliferative capacity, which may provide a source of tendon stem/progenitor cells for tissue engineering. Experts of International Science and Technology Collaborative Program of Ministry of Science and Technology have developed a protocol consensus on differentiation of human embryonic stem cells into the tendon cells. The consensus recommends a protocol of two-step generation of human embryonic stem cells into tendon cells: the human embryonic stem cells are first differentiated into mesenchymal stem cells on different material surfaces; then with the scaffold-free tissue engineering tendon formed by high-density planting, the mesenchymal stem cells are induced into tendon cells under static or dynamic mechanical stimulation in vivo and in vitro. Tissue engineering tendon established in vitro by the protocol can be used as a model in toxicological analysis and safety evaluation of tendon-relevant small molecule compounds, medical materials and drugs.
Key words： Embryonic stem cells    Tendons/cytology    Mesenchymal stem cells    Tissue engineering    Cell differentiation    Cell culture techniques
收稿日期: 2016-02-20     
CLC:  R573.3  
基金资助:

科技部国际科技合作专项重点项目(2011DFA32190)
通讯作者: 欧阳宏伟(1971-),男,博士,教授,博士生导师,从事干细胞与组织工程、运动医学研究;E-mail:hwoy@zju.edu.cn     E-mail: hwoy@zju.edu.cn
作者简介: 陈晓(1982-),男,博士,副教授,博士生导师,从事干细胞与再生医学、肌腱组织工程研究;E-mail:chenxiao-610@zju.edu.cn
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引用本文:

陈晓 等. 人胚胎干细胞向肌腱细胞分化方法的专家共识[J]. 浙江大学学报（医学版）, 2016, 45(2): 105-111.
CHEN Xiao, ZOU Xiaohui, YU Guangyan, FU Xin, CAO Tong, XIAO Yin, OUYANG Hongwei. Expert consensus on induction of human embryonic stem cells into tenocytes. Journal of ZheJiang University(Medical Science), 2016, 45(2): 105-111.

链接本文:

http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2016.03.01      或      http://www.zjujournals.com/xueshu/med/CN/Y2016/V45/I2/105

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