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浙江大学学报（医学版）
浙江大学学报（医学版）  2016, Vol. 45 Issue (1): 86-90,97    DOI: 10.3785/j.issn.1008-9292.2016.01.14
综述     
重组腺病毒载体介导外源性水通道蛋白基因经导管逆行注射治疗干燥综合征研究进展
何虹1, 张洁銎1, 范艳2, 孙晓爽1, 朱玉豪1
1. 浙江大学医学院附属口腔医院, 浙江 杭州 310006;
2. 杭州口腔医院, 浙江 杭州 310006
Transcatheter delivery of recombinant adenovirus vector containing exogenous aquaporin gene in treatment of Sjögren's syndrome
HE Hong1, ZHANG Jieqiong1, FAN Yan2, SUN Xiaoshuang1, ZHU Yuhao1
1. Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China;
2. Hangzhou Stomatology Hospital, Hangzhou 310006, China
全文: PDF(1219 KB)  
摘要: 

腺病毒在感染周期中能最大程度合成病毒蛋白而关闭宿主蛋白质合成,不会引起患者染色体结构破坏,安全性好,基因转染效率也高。人工泪液、唾液或口服激素和免疫抑制剂、酸刺激和手术等等传统疗法的局限性,外源性水通道蛋白转染至干燥综合征小鼠涎腺组织可以改变细胞膜对水的通透性,并将残余导管上皮细胞转变为能分泌水分和盐分的腺泡样细胞,改变细胞的类型与功能,增加腺液分泌,实现涎腺功能重建。上述机制已经得到动物实验证实,美国已经批准应用腺病毒介导的人水通道蛋白对涎腺放射性损伤患者进行I期临床试验。
关键词 ： 水孔蛋白质类,  干燥综合征/代谢,  腺病毒科/遗传学,  重组,遗传,  基因疗法,  综述    
Abstract：

Sjögren's syndrome is a kind of autoimmune disease, whose main clinical symptoms are dry mouth, dry eye and chronic parotid glandular inflammation. The conservative treatments include artificial tears or saliva,oral administration of corticosteroids,and immunosuppressantsl with limited effectiveness. Along with the development of molecular biology, vast attentions are being paid to researches on gene therapy for Sjögren's syndrome, hopefully to bring gospel to patients with Sjögren's syndrome. This article reviews the recent research progresses on transcatheter delivery of recombinant adenovirus vector with aquaporin gene in experimental treatment of Sjögren's syndrome.
Key words： Aquaporins    Sjögren's syndrome/metabolism    Adenoviridae/gentics    Recombination, genetic    Gene therapy    Review
收稿日期: 2015-08-11     
CLC:  R45  
基金资助:

浙江省自然科学基金(Y13H140006);国家卫计委公益性行业专项基金(201502018);国家卫生委省部共建项目(2016139381);国家人力资源部浙江省留学回国人员科研项目(J20120036);浙江省钱江人才项目(20132R10049)
通讯作者: 范艳(1987-),女,硕士,住院医师,主要从事口腔医学研究;E-mail:luolei1214@126.com;http://orcid.org/0000-0003-4839-1023     E-mail: luolei1214@126.com
作者简介: 何虹(1970-),女,博士,主任医师,主要从事口腔医学研究;E-mail:honghehh@zju.edu.cn;http://orcid.org/0000-0003-1299-0868
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何虹 等. 重组腺病毒载体介导外源性水通道蛋白基因经导管逆行注射治疗干燥综合征研究进展[J]. 浙江大学学报（医学版）, 2016, 45(1): 86-90,97.
HE Hong, ZHANG Jieqiong, FAN Yan, SUN Xiaoshuang, ZHU Yuhao. Transcatheter delivery of recombinant adenovirus vector containing exogenous aquaporin gene in treatment of Sjögren's syndrome. Journal of ZheJiang University(Medical Science), 2016, 45(1): 86-90,97.

链接本文:

http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2016.01.14      或      http://www.zjujournals.com/xueshu/med/CN/Y2016/V45/I1/86

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