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浙江大学学报（医学版）
浙江大学学报（医学版）  2015, Vol. 44 Issue (6): 678-683    DOI: 10.3785/j.issn.1008-9292.2015.11.13
综述     
纳秒脉冲电场肿瘤电消融的分子生物学机制
岑超, 陈新华, 郑树森
浙江大学附属第一医院肝胆胰外科, 浙江 杭州 310003
Mechanism of ablation with nanosecond pulsed electric field
CEN Chao, CHEN Xin-hua, ZHENG Shu-sen
Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
全文: PDF(594 KB)  
摘要: 

近年来,纳秒脉冲技术的临床应用研究不断深入与发展,但是其治疗的细胞学机制至今仍未完全阐明。在学术界存在各种假说和理论。研究证实,纳秒脉冲电场可以穿透细胞膜形成纳米孔,引起钙离子内流;也可作用于细胞内膜,引起内质网损伤、线粒体膜电位差的改变;进一步还可以损伤细胞微丝骨架,导致细胞形态的变化。本文结合最新的研究进展,围绕纳秒脉冲电场对细胞膜和细胞器的分子作用机制进行介绍。
关键词 ： 肿瘤/治疗,  电穿孔,  辐射, 电离,  激光,  细胞/辐射效应,  综述    
Abstract：

Nanosecond pulsed electric field ablation has been widely applied in clinical cancer treatment, while its molecular mechanism is still unclear. Researchers have revealed that nanosecond pulsed electric field generates nanopores in plasma membrane, leading to a rapid influx of Ca2+; it has specific effect on intracellular organelle membranes, resulting in endoplasmic reticulum injuries and mitochondrial membrane potential changes. In addition, it may also change cellular morphology through damage of cytoskeleton. This article reviews the recent research advances on the molecular mechanism of cell membrane and organelle changes induced by nanosecond pulsed electric field ablation.
Key words： Neoplasms/therapy    Electroporation    Radiation, ionizing    Lasers    Cells/radiation effects    Review
收稿日期: 2015-06-30 出版日期: 2015-11-12
CLC:  R730.5  
基金资助:

国家自然科学基金(81372425);浙江省自然科学基金(LY13H180003);新疆维吾尔自治区重点实验室专项资金(2014KL002)
通讯作者: 郑树森(1950-),男,博士,教授,主任医师,博士生导师,主要从事肝胆外科工作和肝移植临床研究;E-mail:zyzss@zju.edu.cn;http://orcid.org/0000-0002-9745-5065     E-mail: zyzss@zju.edu.cn
作者简介: 岑超(1989-),男,博士研究生,主要从事纳秒脉冲电场肿瘤电消融的作用机制研究;E-mail:cenchao07@163.com;http://orcid.org/0000-0002-9738-7242
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引用本文:

岑超等. 纳秒脉冲电场肿瘤电消融的分子生物学机制[J]. 浙江大学学报（医学版）, 2015, 44(6): 678-683.
CEN Chao, CHEN Xin-hua, ZHENG Shu-sen. Mechanism of ablation with nanosecond pulsed electric field. Journal of ZheJiang University(Medical Science), 2015, 44(6): 678-683.

链接本文:

http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2015.11.13      或      http://www.zjujournals.com/xueshu/med/CN/Y2015/V44/I6/678

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