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浙江大学学报（医学版）
浙江大学学报（医学版）  2015, Vol. 44 Issue (5): 539-545    DOI: 10.3785/j.issn.1008-9292.2015.09.11
原著     
低频率电刺激抑制杏仁核电点燃癫痫刺激模式依赖效应的实验研究
刘扬1, 汪仪2, 许正浩2,3, 陈忠2
1. 浙江医院神经内科, 浙江杭州 310013;
2. 浙江大学药学院药理系, 浙江杭州 310058;
3. 浙江中医药大学基础医学院, 浙江杭州 310053
Antiepileptic effect of low frequency stimulation in kindling rats
LIU Yang1, WANG Yi2, XU Zheng-hao2,3, CHEN Zhong2
1. Department of Neurology, Zhejiang Hospital, Hangzhou 310013, China;
2. Department of Pharmacology, College of Pharmaceutical Science, Zhejiang University, Hangzhou 310058, China;
3. College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China
全文: PDF(1376 KB)  
摘要: 

目的:研究不同刺激模式的低频率电刺激对杏仁核电点燃癫痫模型大鼠的抗癫痫作用。方法:采用电点燃刺激法建立大鼠癫痫模型,观察开环模式(电点燃前给予)、闭环模式(电点燃后立即给予)以及不同形式闭环模式(全程给予、癫痫早期给予)的低频率电刺激对大鼠癫痫形成过程的影响。结果:闭环模式中全程给予低频率电刺激能降低大鼠电点燃癫痫形成过程中的癫痫发作等级(P<0.01)及缩短后放电持续时间(P<0.05)。闭环模式中癫痫形成早期给予低频率电刺激能降低大鼠电点燃癫痫形成过程中的癫痫发作等级(P<0.05),主要表现在对癫痫0~3级发展过程的抑制(均P<0.05)。而开环模式给予低频率电刺激不能抑制癫痫的发作(P>0.05)。结论:低频率电刺激的抗癫痫作用存在刺激模式依赖效应,这一结果可为深部脑刺激应用于临床治疗癫痫患者提供理论依据。
关键词 ： 电刺激疗法,  癫痫/治疗,  杏仁核,  疾病模型,动物    
Abstract：

Objective:To investigate the antiepileptic effects of various stimulation modes of low-frequency stimulation(LFS) on the kindling rats. Methods:Stimulating electrodes were implanted in the amygdala and current with constant intensity was applied to evoke kindling-induced seizures. The antiepileptic effect of LFS by open loop stimulation(before kindling), closed loop stimulation(immediately after kindling) and different forms of closed loop stimulation(whole stage after kindling and early stage after kindling) were investigated in amygdala kindled rats. Results:The closed loop LFS of whole stage after kindling can significantly inhibited seizure stages(P<0.01) and reduced afterdischarge duration(P<0.05). The closed loop LFS of early stage after kindling can significantly suppress the seizure stages, mainly in stages 0-3(P<0.05 or P<0.01). The open loop low-frequency stimulation did not inhibit the seizure stage during kindling acquisition(P>0.05). Conclusion:The antiepileptic effect of low frequency stimulation may have a mode-dependent effect. It may be helpful for the deep brain stimulation as a promising approach applied to clinical antiepileptic therapy in the future.
Key words： Electric stimulation therapy    Epilepsy/therapy    Amygdala    Disease models, animal
收稿日期: 2015-04-08
CLC:  R74  
基金资助:

国家自然科学基金(81302749)
通讯作者: 陈忠(1968-),男,博士,教授,博士生导师,主要从事神经药理学研究;E-mail:chenzhong@zju.edu.cn;http://orcid.org/0000-0003-4755-9357     E-mail: chenzhong@zju.edu.cn
作者简介: 刘扬(1985-),女,博士,住院医师,主要从事癫痫的发病与治疗学研究;E-mail:kingofhades@vip.sina.com;http://orcid.org/0000-0003-1946-3972
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引用本文:

刘扬, 汪仪, 许正浩, 陈忠. 低频率电刺激抑制杏仁核电点燃癫痫刺激模式依赖效应的实验研究[J]. 浙江大学学报（医学版）, 2015, 44(5): 539-545.
LIU Yang, WANG Yi, XU Zheng-hao, CHEN Zhong. Antiepileptic effect of low frequency stimulation in kindling rats. Journal of ZheJiang University(Medical Science), 2015, 44(5): 539-545.

链接本文:

http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2015.09.11      或      http://www.zjujournals.com/xueshu/med/CN/Y2015/V44/I5/539

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