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浙江大学学报（医学版）
浙江大学学报（医学版）  2015, Vol. 44 Issue (2): 184-188    DOI: 10.3785/j.issn.1008-9292.2015.03.011
原著     
神经元特异性烯醇化酶和突触素在人胚胎食管组织发育阶段的表达
刘学红, 张泳, 刘传康
绍兴文理学院医学院, 浙江 绍兴 312000
Expression of neuron-specific enolase and synaptophysin in esophageal development of human embryos
LIU Xue-hong, ZHANG Yong, LIU Chuan-kang
School of Medicine, Shaoxing University, Shaoxing 312000, China
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摘要：

目的:探讨神经元特异性烯醇化酶(NSE)和突触素(SYN)蛋白在人胚胎食管组织不同发育阶段的分布特征。方法:经伦理委员会批准,收集受精龄为7～16周的人胚胎标本16例,应用免疫组织化学染色法检测胚胎食管组织内NSE和SYN的表达,定量分析其变化规律。结果:第2～4个月胎龄时,肌间神经丛和黏膜下神经丛内细胞呈NSE阳性表达,阳性表达的数量和强度第3个月胎龄甚于第2个月胎龄,第4个月胎龄甚于第3个月胎龄。仅在第3个月胎龄时,食管黏膜上皮层内部分细胞呈NSE阳性表达。第2～4个月胎龄时,SYN在食管各层组织内均呈广泛阳性或强阳性表达,尤其在肌间神经丛和黏膜下神经丛更为明显。结论:在人胚胎食管发育阶段, NSE和SYN均参与食管壁神经系统的发育过程。
关键词： 食管/病理学;  食管/胚胎学;  磷酸丙酮酸水合酶;  突触蛋白类;  免疫组织化学    
Abstract:

Objective: To investigate the expression of neuron-specific enolase (NSE) and synaptophysin(SYN) proteins in different developmental stages of human embryonic esophagus. Methods: Immunohistochemistry was used to detect the expressions of NSE and SYN proteins in embryonic esophagus tissues of fetuses of 2, 3 and 4 month gestational age (n=16). One-way ANOVA and LSD-t test were employed to compare the staining intensity and number of positive expression cells in embryonic esophageal tissues of different gestational age. Results: In fetuses with 2, 3 and 4 months of gestation, the number of NSE-positive nerve cells in the myenteric nerve plexus and submucosa of human embryonic esophageal tissues were 18.38±8.37, 25.00±11.54 and 38.00±15.09, respectively; the staining intensity of NSE-positive nerve cells and nerve fibers in myenteric nerve plexus and submucosa of embryonic esophageal tissues were 74.38±14.93, 62.25±18.59 and 56.44±14.70, respectively. NSE-positive cells were detected in the esophageal epithelium only at the third month. In the fetuses at 2, 3 and 4 months of gestation, SYN in all layers of esophageal tissue were positively or strong positively expressed, especially in the myenteric plexus and submucosal plexus. The staining intensity of SYN-positive cells in embryonic esophagus tissues of 2, 3 and 4 month gestation were 54.69±9.34, 51.84±6.10 and 46.41±6.44, respectively. Conclusion: SYN and NSE may be involved in the regulation of nerve system of esophageal tissues during the human embryonic development.
Key words: Esophagus/patholoy    Esophagus/embryology    Phosphopyruvate hydratase    Synapsins    Immunohistochemistry
收稿日期: 2014-04-10 出版日期: 2015-03-25
:  R329.1  
基金资助:

浙江省教育厅科研计划(Y201018535)
作者简介: 刘学红(1979-),男,硕士,副教授,主要从事胚胎中枢神经系统及消化系统组织发育研究;E-mail:liuxueh6588@126.com
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引用本文:

刘学红, 张泳, 刘传康. 神经元特异性烯醇化酶和突触素在人胚胎食管组织发育阶段的表达[J]. 浙江大学学报（医学版）, 2015, 44(2): 184-188.

LIU Xue-hong, ZHANG Yong, LIU Chuan-kang. Expression of neuron-specific enolase and synaptophysin in esophageal development of human embryos. Journal of ZheJiang University(Medical Science), 2015, 44(2): 184-188.

链接本文:

http://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.2015.03.011    或    http://www.zjujournals.com/med/CN/Y2015/V44/I2/184

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