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浙江大学学报（医学版）
浙江大学学报（医学版）  2015, Vol. 44 Issue (1): 49-53    DOI: 10.3785/j.issn.1008-9292.2015.01.008
专题报道     
穿山龙醇提取物对高尿酸血症小鼠的治疗作用
单海丽1, 单瑞平2, 傅旭春3
1. 浙江大学医学院附属第二医院药剂科, 浙江 杭州 310009;
2. 浙江万晟药业有限公司, 浙江 杭州 310013;
3. 浙江大学城市学院药物研究所, 浙江 杭州 310015
Hypouricemic effect of ethanol extracts from Dioscoreae Nipponicae Rhizoma
SHAN Hai-li1, SHAN Rui-ping2, FU Xu-chun3
1. Department of Pharmacy, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China;
2. Zhejiang Wansheng Pharmaceutical Co. Ltd., Hangzhou 310013, China;
3. Institute of Materia Medica, Zhejiang University City College, Hangzhou 310015, China
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摘要：

目的:研究穿山龙醇提取物对高尿酸血症小鼠的降血尿酸作用. 方法:分别用次黄嘌呤灌胃同时皮下注射氧嗪酸钾(模型A)和皮下注射尿酸(模型B)的方法建立两种小鼠高尿酸血症模型,并用穿山龙醇提取物干预两种模型,评估其降尿酸的效果.用高效液相色谱法测定血尿酸含量. 结果:对于次黄嘌呤和氧嗪酸钾诱导的小鼠高尿酸血症模型,模型A组、空白对照组和穿山龙醇提取物组的血尿酸水平分别为(40.03±27.24)、(4.08±1.47)和(18.10±8.87)g/mL,穿山龙醇提取物组与模型组比较差异有统计学意义(P <0.05);对于尿酸诱导的小鼠高尿酸血症模型,模型B组、空白对照组和穿山龙醇提取物组的血尿酸水平分别为(18.57±3.83)、(4.29±2.36)和(15.36±2.71)g/mL,穿山龙醇提取物组与模型组比较差异有统计学意义(P <0.05).结论:对于上述两种高尿酸血症动物模型,穿山龙醇提取物组均有显著的降血尿酸作用.
关键词： 穿山龙/治疗应用;  中草药提取物;  高尿酸血症/药物疗法;  次黄嘌呤;  尿酸;  疾病模型, 动物    
Abstract:

Objective: To investigate the effect of ethanol extracts from Dioscoreae Nipponicae Rhizoma on hyperuricemic mice. Methods: The hyperuricemia was induced by gavage of hypoxanthine and subcutaneous injection of potassium oxonate (model A) or subcutaneous injection of uric acid (model B) in ICR male mice. The mice in ethanol extracts groups were administrated with Dioscoreae Nipponicae Rhizoma ethanol extracts 5.4 g/kg by gavage, the positive control groups were given with 10 mg/ml allopurinol or 5 mg/ml benzbromarone by gavage, respectively. The plasma uric acid levels were measured by using HPLC. Results: The plasma uric acid levels of model group, control group and ethanol extract group in model A mice were (40.03±27.24), (4.08±1.47) and (18.10±8.87) g/mL(compared with model group, P <0.05), respectively. The plasma uric acid levels of model group, control group and ethanol extract group in model B mice were (18.57±3.83), (4.29±2.36) and (15.36±2.71) g/mL (compared with model group, P <0.05), respectively. Conclusion: The ethanol extracts from Dioscoreae Nipponicae Rhizoma have certain hypouricemic effect in hyperuricemic mice induced by hypoxanthine and potassium oxonate or by uric acid.
Key words: DIOSCOREA NIPPONICA/therapeutic use    Drugs, Chinese herbal    Hyperuricemia/drug therapy    Hypoxanthine    Uric acid    Disease models, animal
收稿日期: 2014-05-21 出版日期: 2015-03-25
CLC:  R96  
基金资助:

杭州市科技发展计划项目(20100331T01).
通讯作者: 傅旭春(1960-),男,博士,教授,主要从事新药研发和药物动力学研究;E-mail: fuxc@zucc.edu.cn     E-mail: fuxc@zucc.edu.cn
作者简介: 单海丽(1984-),女,硕士,药师,主要从事药理学与临床药学研究;E-mail: 251787862@qq.com
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单海丽, 单瑞平, 傅旭春. 穿山龙醇提取物对高尿酸血症小鼠的治疗作用[J]. 浙江大学学报（医学版）, 2015, 44(1): 49-53.

SHAN Hai-li, SHAN Rui-ping, FU Xu-chun. Hypouricemic effect of ethanol extracts from Dioscoreae Nipponicae Rhizoma. Journal of ZheJiang University(Medical Science), 2015, 44(1): 49-53.

链接本文:

http://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.2015.01.008    或    http://www.zjujournals.com/med/CN/Y2015/V44/I1/49

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