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浙江大学学报（医学版）
浙江大学学报（医学版）  2014, Vol. 43 Issue (4): 397-405    DOI: 10.3785/j.issn.1008-9292.2014.06.005
专题报道     
分泌型卷曲相关蛋白1、β联蛋白、上皮钙黏着蛋白在结直肠癌中的表达及意义
欧玉荣1，刘娟1，高杉2，景桂英1，承泽农1，董修琴1
1. 蚌埠医学院第一附属医院病理科 蚌埠医学院病理学教研室，安徽 蚌埠233004 2. 安徽医科大学药理学教研室，安徽 合肥 230032
Expression of secreted frizzled related protein 1，β-catenin and E-cadherin in colorectal carcinoma and its clinicopathological significances
OU Yu-rong1， LIU Juan1， GAO Shan2， JING Gui-ying1， CHENG Ze-nong1， DONG Xiu-qin1
1. Department of Pathology， First Affiliated Hospital of Bengbu Medical College， Bengbu 233004， China; 2. Department of Pharmacology， College of Basic Medical， Anhui Medical University， Hefei 230032， China
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摘要: 目的： 探讨Wnt信号通路抑制因子分泌型卷曲相关蛋白1（SFRP1）在结直肠癌组织中的表达，及其与β联蛋白（β-catenin）和上皮钙黏着蛋白（E-cadherin）蛋白表达的关系，并探讨其临床意义。 方法：应用逆转录PCR检测60份结直肠癌及其癌旁正常黏膜中SFRP1、β-catenin和E-cadherin基因mRNA表达水平；同时应用免疫组织化学染色法（Elivision法）检测相同组织中SFRP1、β-catenin和E-cadherin蛋白的表达，并探讨其相关性及与临床病理因素的关系。 结果：60份组织中，52份结直肠癌组织及其癌旁正常黏膜中RNA提取成功；SFRP1 mRNA相对表达量分别为0.4837±0.1532和0.7170±0.1830；β-catenin mRNA相对表达量分别为0.9293±0.3705和0.6469±0.3166；E-cadherin mRNA相对表达量分别为0.5556±0.2535和0.9422±0.2372，两组比较差异均有统计学意义（均P<0.01）；SFRP1 mRNA表达与肿瘤淋巴结转移有关（P<0.05）。SFRP1蛋白在结直肠癌中的阳性表达率为31.67%（19/60），显著低于癌旁正常结直肠黏膜组织75.00%（45/60）的阳性表达率；SFRP1 蛋白表达与临床病理各因素无关。β-catenin、E-cadherin在结直肠癌中的异常表达率分别为75.00%（45/60）、58.33%（35/60），显著高于癌旁正常结直肠黏膜组织1.67%（1/60）、6.67%（4/60）的异常表达率；β-catenin和E-cadherin异常表达与肿瘤的分化程度、淋巴结转移和Dukes分类有关。结直肠癌中SFRP1蛋白的表达与β-catenin和E-cadherin的异常表达呈负相关（r=－0.517，r=－0.442， 均P＜0.01）。 结论：结直肠癌中SFRP1的表达下调导致Wnt信号通路出现异常活化，并引起β-catenin和E-cadherin异常表达增加。提示SFRP1失活及水平下调是结直肠癌启动及促进的重要因素。
Abstract：Objective： To investigate the expression of secreted frizzled-related protein 1 （SFRP1）， β-catenin and E-cadherin in colorectal carcinoma and its clinicopathological significance. Methods： The expression of SFRP1， β-catenin and E-cadherin mRNA and protein in tumor and pericancerous tissue samples from 60 cases of colorectal cancer was assayed by reverse-transcription PCR and immunohistochemistry， respectively. The correlation of their expression with clinicopathological factors of colorectal cancer was analyzed. Results： In 52/60 cases the relative mRNA expression of SFRP1 in cancer tissue and pericancerous tissue was 0.4837 ± 0.1532 and 0.7170 ± 0.1830; for β-catenin was 0.9293 ± 0.3705 and 0.6469±0.3166; and for E-cadherin was 0.5556±0.2535 and 0.9422±0.2372 （P<0.01）， respectively. SFRP1 mRNA expression was associated with lymphatic metastasis （P<0.05）. The positive rate of SFRP1 in colorectal cancer was 31.67% （19/60）， and was significantly lower than that in pericancerous colorectal mucosa （75.00%， 45/60）. No relationship between SFRP1 protein expression and clinical pathology was found. Abnormal expression rates of β-catenin and E-cadherin in colorectal cancer were 75.00% （45/60） and 58.33% （35/60）, respectively， which were significantly higher than that in pericancerous colorectal mucosa （1.67% and 6.67%）， respectively. Abnormal β-catenin and E-cadherin expression was associated with tumor differentiation， lymphatic metastasis and Dukes staging. SFRP1 protein expression was negatively correlated with β-catenin and E-cadherin expression （r=-0.517,-0.442,Ps<0.01）. Conclusion： Down-regulation of SFRP1 in colorectal cancer may cause abnormal Wnt signaling and induce abnormal β-catenin and E-cadherin expression， indicating that SFRP1 might be involved in the development and progression of colorectal cancer， and could be a novel therapeutic target for colorectal cancer．
收稿日期: 2014-01-10
基金资助:国家自然科学基金面上项目（81073088）；安徽省教育厅自然科学研究项目（2010 KJ116B）；蚌埠市科技局科研项目（200903020）．
作者简介: 欧玉荣（1974-）， 女， 副教授，硕士生导师，从事消化道肿瘤病理研究;E-mail: snowyc1220@163.com
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引用本文:

欧玉荣，等. 分泌型卷曲相关蛋白1、β联蛋白、上皮钙黏着蛋白在结直肠癌中的表达及意义[J]. 浙江大学学报（医学版）, 2014, 43(4): 397-405.
OU Yu-rong，et al. Expression of secreted frizzled related protein 1，β-catenin and E-cadherin in colorectal carcinoma and its clinicopathological significances . Journal of ZheJiang University(Medical Science), 2014, 43(4): 397-405.

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http://www.zjujournals.com/xueshu/med/CN/10.3785/j.issn.1008-9292.2014.06.005      或      http://www.zjujournals.com/xueshu/med/CN/Y2014/V43/I4/397

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