摘要: 目的：检测前B细胞白血病转录因子-2（PBX2）/ Ets家族转录因子-2 （ELF2）的表达，探讨PBX2/ELF2表达与非小细胞肺癌（NSCLC）预后的关系。
方法：对206例NSCLC手术切除标本进行ELF2、PBX2的免疫组织化学染色，采用χ2检验、Fisher确切检验、Coxs回归等统计学方法分析比较PBX2/ELF2共表达与缬酪肽包含蛋白（VCP）表达、肿瘤浸润、转移、患者预后等的关系。
结果：PBX2/ELF2表达与VCP表达高度相关（P=0.0126）。单变量分析PBX2表达、PBX2/ELF2表达、VCP表达、肿瘤直径、组织分化程度、胸膜转移、浸润深度、区域淋巴结转移、临床分期与NSCLC患者5年无病生存率、总体生存率相关（均P<0.05），5年总体生存率与血管侵袭也相关（P=0.0322）；多变量分析显示PBX2/ELF2表达、组织分化程度是NSCLC预后的独立相关因子。
结论：PBX2/ELF2表达与VCP表达呈正相关，PBX2/ELF2-VCP通路与NSCLC患者预后相关。
关键词 ：
PBX2丨ELF2丨非小细胞肺/病理学丨转录因子丨基因 ,
同源盒丨基因表达丨预后丨免疫组织化学
Abstract ：Objective： To investigate the relationship between pre B cell leukemia transcription factor 2 （PBX2）/Ets domain transcription factor 2 （ELF2 ） expression with prognosis of non-small cell lung cancer （NSCLC）.
Methods： Expressions of ELF2 and PBX2 were examined in 206 patients of NSCLC by immunohistochemistry. The correlation of PBX2/ELF2 expression with valosin-containing protein （VCP） expression and clinicopathologic factors of NSCLC patients was analyzed. Chi-square test， Fishers exact test and Coxs regression were used for statistical analysis.
Results： The level of PBX2/ELF2 expression was associated with VCP expression （P=0.0126）. Univariate analysis showed that 5-y disease free survival and overall survival （OS） of NSCLC were correlated with expression of PBX2， PBX2/ELF2 and VCP， tumor size， histological differentiation， visceral pleural invasion， N and T in pTNM grades， and clinical stages （P<0.05）. The 5-y OS was also related to vascular invasion （P=0.0322）. Multivariate analysis revealed that the expression level of PBX2/ELF2 and histological differentiation were independent predictors for NSCLC.
Conclusion： The level of PBX2/ELF2 expression is related to VCP expression， indicating that PBX2/ELF2-VCP pathway may be associated with the prognosis of NSCLC patients．
收稿日期: 2013-08-16
基金资助: 国家自然科学基金青年科学基金（81200968）.
通讯作者:
裘莹（1973－），女，博士，副教授，从事肿瘤学研究；E-mail∶yingqiu@biomed.tsinghua.edu.cn
Corresponding author:
QIU Ying，E-mail: yingqiu@biomed.tsinghua.edu.cn
作者简介 : 金淑清（1989－），女，硕士研究生，从事肺损伤研究；E-mail∶jinshuqing1@163.com
