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浙江大学学报(医学版)  2013, Vol. 42 Issue (6): 632-637    DOI: 10.3785/j.issn.1008-9292.2013.06.007
专题报道     
苍耳水提物抑制α-葡萄糖苷酶活性及降低小鼠血糖的作用
郭凤霞1,3,曾阳2,3,李锦萍2,3
1.浙江大学城市学院医学院,浙江 杭州 310015;
2.青海师范大学生命与地理科学学院,
3.青藏高原环境与资源教育部重点实验室,青海 西宁 810008;
Inhibition of α-glucosidase activity by water extracts of Xanthium sibiricum Patrin ex Widder and their effects on blood sugar in mice
GUO Fengxia1,2,ZENG Yang2,3,LI Jingping2,3
1.Zhejiang University City College, School of Medicine, Hangzhou 310015,China; 2.College of Biological and Geographical Science,Qinghai Normal University,3.Key Laboratory of Environment and Resources of Qinghai-Tibet Plateau under the National Education Ministry,Xining 810008,China;
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摘要:

目的:探讨苍耳水提物(CEW)对 α-葡萄糖苷酶(AG)抑制活性及对正常小鼠和实验性高血糖小鼠血糖的影响。
方法:利用酶-抑制剂体外筛选模型,阿卡波糖(Acarbose)为对照品,不同浓度的CEW进行酶抑制活性研究。体内试验,CEW以40.0、10.0 g·kg-1,阿卡波糖0.375 g·kg-1,生理盐水(NaCL)0.3ml给正常小鼠ig,连续5 d后,分别负荷葡萄糖2.0 g·kg-1、蔗糖4.0 g·kg-1、淀粉2.0 g·kg-1,在15、30、60、120 min时间点测定小鼠血糖值。以链脲佐菌素(STZ)诱导小鼠糖尿病模型,CEW以40.0、10.0 g·kg-1,二甲双胍片(Metformin)0.08g·kg-1,参芪胶囊(SSJN)0.30 g·kg-1给正常小鼠ig,测定连续给药2周和4周后的血糖值。
结果:酶-抑制剂模型中,CEW浓度在0.3125 g·L-1~10.00 g·L-1时,抑制率为55.42%~92.73%;阿卡波糖浓度为1.5625 g·L-1~25.00 g·L-1时,抑制率为9.28%~64.87%。耐糖量试验中,淀粉组血糖值降低明显(P<0.01),蔗糖组次之(P<0.05),葡萄糖组无统计学意义(P>0.05)。小鼠糖尿病模型试验中,连续给药2周后, CEW 40.0g·kg-1(CEW-40)组对糖尿病小鼠降糖作用极其显著(P<0.01), CEW10.0g·kg-1(CEW-10)组降糖作用显著降低(P<0.05),4周后,两组降糖作用均极其显著(P<0.01)。
结论:CEW中含有α-葡萄糖苷酶抑制剂(AGI)的活性成分,抑制AG活性的作用强于阿卡波糖,浓度在0.3125 g·L-1~10.00 g·L-1范围内,抑制率与浓度成正比,可提高正常小鼠的耐糖量;高血糖小鼠模型试验中,CEW可降低糖尿病小鼠血糖。

关键词: 苍耳子/治疗应用 糖尿病/药物疗法 植物提取物/药理学降血糖药(中药)/治疗应用 苍耳水提物&alpha-葡萄糖苷酶 抑制剂 糖尿病动物模型 降血糖作用    
Abstract:

Objective: To investigate the effects of Xanthium sibiricum Patrin ex Widder water extracts (CEW) on α-glucosidase activity (AG) and blood sugar in mice.
Methods: The inhibition of AG by CEW was studied with enzyme-inhibitor screening external model with acarbose as control drug.The normal mice were administrated by gavage with 40.0 g·kg-1,or 10.0 g·kg-1 of CEW,0.375 g·kg-1 of acarbose,and 0.3ml of normal saline,respectively in successive 5 days; then the animals were loaded with 2.0 g·kg-1 of glucose,4.0 g·kg-1 of sucrose,and 2.0 g·kg-1 of starch and blood sugar levels were measured within 15,30,60,and 120min.Diabetes was induced by injection of streptozotocin (STZ) in mice,then 40.0 g·kg-1,or 10.0 g·kg-1 of CEW was given to diabetic mice in successive 2 weeks and 4 weeks,then the blood sugar levels were measured.
Results: In the enzyme inhibition test,when the concentration of CEW was between 0.3125 g·L-1-10.00 g·L-1,the inhibition rate was 55.42%-92.73%; when the concentration of acarbose was 1.5625 g·L-1-25.00 g·L-1,the inhibition rate was 9.28%-64.87%.In the sugar tolerance test,the blood sugar value in starch-loaded mice decreased sharply (P<0.01),followed by sucrose-loaded group (P<0.05),and there was no change in glucose-loaded group (P>0.05).In diabetic mice CEW-40 and CEW-10 groups showed significant blood sugar lowering effect (P<0.01 or P<0.05).
Conclusions: CEW has stronger effect in inhibition of AG activity than acarbose.CEW can increase the sugar tolerance in normal mice and decrease the blood sugar level in diabetic mice.

Key words: FRUCTUS XANTHII/therapeutic use    Diabetes mellitus/drug therapy    Plant extrancts/pharmacology    HYPOGLYCEMIC AGENTS (TCD)/therapeutic use    Water extracts of X.sibiricum Patrin ex Widder    Alpha-glucosidase enzyme    Inhibitors    Diabetes animal model    Blood sugar reduction
收稿日期: 2013-05-07 出版日期: 2013-11-25
基金资助:

青海省自然科学基金项目(2012-Z-909).

作者简介: 郭凤霞(1970-),女,硕士,副教授,研究方向:天然药物有效物质的提取及其药理活性研究; Email:guofengx @ zucc.edu.cn
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引用本文:

郭凤霞,曾阳,李锦萍. 苍耳水提物抑制α-葡萄糖苷酶活性及降低小鼠血糖的作用[J]. 浙江大学学报(医学版), 2013, 42(6): 632-637.

GUO Fengxia,ZENG Yang,LI Jingping. Inhibition of α-glucosidase activity by water extracts of Xanthium sibiricum Patrin ex Widder and their effects on blood sugar in mice. Journal of ZheJiang University(Medical Science), 2013, 42(6): 632-637.

链接本文:

https://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.2013.06.007        https://www.zjujournals.com/med/CN/Y2013/V42/I6/632

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