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浙江大学学报（医学版）
浙江大学学报（医学版）  2013, Vol. 42 Issue (5): 556-560    DOI: 10.3785/j.issn.1008-9292.2013.05.013
论著     
豨莶草对单克隆抗体组合诱导的小鼠类风湿关节炎作用
傅旭春1*，蒋芳萍2，3，范珈祯1，白海波3
1.浙江大学城市学院药物研究所，浙江 杭州 310015；
2.浙江大学药学院药学系，浙江 杭州 310058；
3.杭州华东医药集团生物工程研究所有限公司，浙江 杭州 310011；
Extract of Herba Siegesbeckiae on mouse rheumatoid arthritis induced by anthrogen-CIA monoclonal antibody
FU Xu-Chun1*, JIANG Fang-Ping2，3, FAN Jia-Zhen1, BAI Hai-Bo3
1.Institute of Materia Medica，Zhejiang University City College，Hangzhou 310015,China；2.Department of Pharmacy, College of Pharmaceutical Sciences，Zhejiang University，Hangzhou 310058,China; 3.Hangzhou Huadong Medicine Group Biotechnology R&D Institute Co.Ltd.，Hangzhou 310011，China；
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摘要：

目的：观察豨莶草对单克隆抗体组合诱导类风湿关节炎小鼠的抗炎作用。
方法：采用单克隆抗体组合诱导建立小鼠类风湿动物模型，用夹心法酶联免疫吸附测定试剂盒测定小鼠血清中白介素-1β（IL-1β）、后肢膝关节以下组织匀浆上清液中白介素-6（IL-6）、白介素-17（IL-17）、基质金属蛋白酶-3（MMP-3）的含量。
结果：用药21 d后模型组和豨莶草组足趾容积分别为（0.2545±0.0179）cm3和（0.218±0.0307）cm3（P<0.05），血清IL-1β水平、组织上清液中IL-6、IL-17、MMP-3含量分别为（104.96±31.22）pg/ml和（63.74±21.74）pg/ml（P<0.01）、（249.64±75.08）pg/ml和（171.10±48.35）pg/ml（P<0.05）、（208.40±88.54）pg/ml和（115.42±56.52）pg/ml（P<0.05）、（5420.79±1201.43）和（3660.31±1680.99）pg/ml（P<0.05)，豨莶草提取物能减轻单克隆抗体组合诱导的类风湿关节炎小鼠的足趾肿胀，，降低血清和组织中IL-1β、IL-6、IL-17、MMP-3的表达水平。
结论：豨莶草提取物对单克隆抗体组合诱导类风湿关节炎小鼠具有抗炎作用。
关键词： 关节炎,类风湿;  豨莶草;  白细胞介素1β;  白细胞介素6;  白细胞介素17;  基质金属蛋白酶3;  疾病模型,动物    
Abstract:

Objective： To investigate the anti-inflammatory effect of Herba Siegesbeckiae extracts on mouse rheumatoid arthritis induced by arthrogen-CIA monoclonal antibody.
Methods： The rheumatoid arthritis was induced by arthrogen-CIA arthritogenic monoclonal antibody in mice.The sandwich enzyme-linked immunosorbent assay was used to determine the concentration of IL-1β in mouse serum，and the content of IL-6，IL-17 and MMP-3 in supernatant of tissue homogenate of hind limb below the stifle of mice.One-way ANOVA was used for data analysis.
Results： The toe swelling was attenuated in Siegesbeckiae group than that in model group [(0.218±0.0307)cm3 vs (0.2545±0.0179)cm3，P<0.05].The serum IL-1β level in Siegesbeckiae group was lower than that in model group [(63.74±21.74)pg/ml vs (104.96±31.22)pg/ml，P<0.01].The contents of IL-6，IL-17 and MMP-3 in tissue supernatants of Siegesbeckiae group were all lower than those of model group [(171.10±48.35)pg/ml vs (249.64±75.08)pg/ml，P<0.05; (115.42±56.52)pg/ml vs (208.40±88.54)pg/ml，P<0.05; (3660.31±1680.99)pg/ml vs (5420.79±1201.43)pg/ml，P<0.05，respectively].
Conclusion： The extract of Herba Siegesbeckiae has anti-inflammatory effect on mouse rheumatoid arthritis induced by mixed arthrogen monoclonal antibody.
Key words: Arthritis, Rheumatoid    Siegesbeckia orientalis    Interleukin-1beta    Interleukin-6    Interleukin-17    Matrix metalloproteinase 3    Disease models, Animal
收稿日期: 2013-06-14 出版日期: 2013-09-25
:  R 742.1  
基金资助:

杭州市科技发展计划项目（20100331T01）.
作者简介: 傅旭春 (1960-)，男，博士，教授，主要从事新药研发和药物动力学研究；E-mail:fuxc@zucc.edu.cn
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引用本文:

傅旭春, 蒋芳萍, 范珈祯, 白海波. 豨莶草对单克隆抗体组合诱导的小鼠类风湿关节炎作用[J]. 浙江大学学报（医学版）, 2013, 42(5): 556-560.

FU Xu-Chun, JIANG Fang-Ping, FAN Jia-Zhen, BAI Hai-Bo. Extract of Herba Siegesbeckiae on mouse rheumatoid arthritis induced by anthrogen-CIA monoclonal antibody. Journal of ZheJiang University(Medical Science), 2013, 42(5): 556-560.

链接本文:

http://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.2013.05.013    或    http://www.zjujournals.com/med/CN/Y2013/V42/I5/556

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