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浙江大学学报（医学版）
浙江大学学报（医学版）  2013, Vol. 42 Issue (2): 177-183    DOI: 10.3785/j.issn.1008-9292.2013.02.008
论著     
辣椒素对大鼠肠系膜阻力血管的舒张作用及其机制
陈强1，2，朱欢欢1，张媛媛1，张媛1，王利宏1，郑良荣1
1.浙江大学医学院附属第一医院心内科，浙江 杭州 310003；
2.浙江大学医学院附属义乌医院心内科，浙江 义乌 322000；
Vasodilating effect of capsaicin on rat mesenteric artery and its mechanism
CHEN Qiang1，2, ZHU Huan-Huan1, ZHANG Yuan-Yuan1, ZHANG Yuan1, WANG Li-Hong1, ZHENG Liang-Rong1
1.Department of Cardiology，The First Affiliated Hospital，Zhejiang University School of Medicine，Hangzhou 310003，China; 2.Department of Cardiology,The Yiwu Affiliated Hospital,Zhejiang University School of Medicine,Yiwu 322000,China)
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摘要：

目的：观测辣椒素（capsaicin，CAP）对大鼠肠系膜阻力血管的舒张作用，探讨其可能机制。
方法：雄性Sprague-Dawley大鼠，取肠系膜动脉三级分支约2 mm长血管环，置于DMT 610M系统，记录张力变化。
结果：CAP（10-9~10-5 mol/L）对苯肾上腺素（PE）预收缩的内皮完整血管和去内皮血管产生浓度依赖性的舒张作用，其中内皮完整组的血管舒张作用比去内皮组更明显，两者相比CAP在10-7~10-5 mol/L时，差异有统计学意义（P<0.01）。该舒张作用可被一氧化氮合酶抑制剂L-NAME和CGRP竞争性阻滞剂CGRP8-37阻断。CGRP（10-10~3×10-8 mol/L）对PE预收缩的内皮完整血管和去内皮血管产生浓度依赖性的舒张作用，其中内皮完整组的血管舒张作用比去内皮组更明显，两者相比CGRP在3×10-9~3×10-8 mol/L时，差异有统计学意义（P<0.05）。P物质对PE预收缩的内皮完整血管无舒张作用。
结论：CAP对大鼠肠系膜阻力动脉具有部分内皮依赖性舒张作用，该作用机制与其促进内皮一氧化氮合酶（NOS）途径有关；CAP的非内皮依赖性舒张血管效应与CGRP释放有关。
关键词： 辣椒素/药理学;  肠系膜动脉/药物作用;  血管舒张;  降钙素基因相关肽;  内皮    
Abstract:

Objective： To investigate the vasodilating effect of capsaicin(CAP) on rat mesenteric artery and its mechanism.
Methods： The third branch of the superior mesenteric artery in male Sprague-Dawley rat(250-350 g) was excised，the periadventitial fat and connective tissue were removed and the mesenteric artery was dissected into 2 mm rings.Each ring was placed in a 5 ml organ bath of DMT 610M system and the tension was recorded.
Results： CAP(10-9-10-5 mol/L) relaxed endothelium-intact and endothelium-denuded mesenteric artery pre-constricted by phenylephrine(10-5 mol/L)，and the vasodilation in endothelium-intact mesenteric artery was stronger than that in endothelium-denuded one.Pretreatment with either L-NAME(3×10-4 mol/L)，an inhibitor of nitric oxide synthase(NOS)， or CGRP8-37(2×10-6 mol/L)，an antagonist of calcitonin gene-related peptide(CGRP)，for 30 min significantly attenuated the relaxation of endothelium-intact mesenteric artery induced by CAP.CGRP(10-10-3×10-8 mol/L) relaxed endothelium-intact and endothelium-denuded mesenteric artery pre-constricted by phenylephrine，and the vasodilation in endothelium-intact mesenteric artery was stronger than that in endothelium-denuded one.Substance P did not relax the mesenteric artery pre-constricted by phenylephrine.
Conclusions： CAP has partial endothelium-dependent relaxation effect on rat mesenteric artery，which may be mediated by activating the endothelial NOS-NO pathway.The endothelium-independent relaxation in rat mesenteric artery induced by CAP may be mediated by CGRP.
Key words: Capsaicin/pharmacology    Mesenteric arteries/drug effects    Vasodilation    Calcitonin gene-related peptide    Endothelium
收稿日期: 2012-04-27 出版日期: 2013-03-25
:  R 331.3  
基金资助:

国家自然科学基金项目（81170167 ）；浙江省重大科技专项计划项目（2011C14027）；浙江省中医药优秀青年人才基金计划（2011ZQ013）.
通讯作者: 郑良荣（1963-），男，博士，主任医师，主要从事心血管病学研究     E-mail: zlrylnn@126.com
作者简介: 陈强（1985-），男，心血管内科硕士研究生.
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陈强
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引用本文:

陈强, 朱欢欢, 张媛媛, 张媛, 王利宏, 郑良荣. 辣椒素对大鼠肠系膜阻力血管的舒张作用及其机制[J]. 浙江大学学报（医学版）, 2013, 42(2): 177-183.

CHEN Qiang, ZHU Huan-Huan, ZHANG Yuan-Yuan, ZHANG Yuan, WANG Li-Hong, ZHENG Liang-Rong. Vasodilating effect of capsaicin on rat mesenteric artery and its mechanism. Journal of ZheJiang University(Medical Science), 2013, 42(2): 177-183.

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http://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.2013.02.008    或    http://www.zjujournals.com/med/CN/Y2013/V42/I2/177


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