Please wait a minute...
浙江大学学报(医学版)  2012, Vol. 41 Issue (5): 559-563    DOI: 10.3785/j.issn.1008-9292.2012.05.015
论著     
硫化氢预处理对大鼠心肌缺血再灌注损伤的保护作用
李辉1,冉珂1,唐正国2,李双凤1,常业恬1
1.中南大学湘雅二医院麻醉科,湖南 长沙 410011; 2.长沙市第三医院麻醉科,湖南 长沙 410015
Effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats
LI Hui1,RAN Ke1,TANG Zheng-guo2,LI Shuang-feng1,CHANG Ye-tian1
1.Department of Anesthesiology,Second Xiangya Hospital,Central South University,Changsha 410011,China; 2.Department of Anesthesiology,the Third Changsha Municipal Hospital,Changsha 410015,China
 全文: PDF(1038 KB)   HTML (
摘要: 目的:探讨硫化氢预处理对大鼠心肌缺血再灌注损伤的保护作用。
方法:健康成年SD雄性大鼠40只,随机分成4组,每组10只。假手术组(S组)仅开胸并分离冠状动脉左前降支,不阻断血流150 min;缺血再灌注组(IR组)行冠状动脉左前降支阻断30 min,再灌注120 min;硫化氢预处理组(H组)予以静脉注射硫化氢钠0.05 mg/kg,给药后24 h同IR组处理;硫化氢预处理+线粒体KATP通道阻断剂(5-羟葵酸,5-HD)组(D组)缺血前15 min静脉注射5-HD 5 mg/kg,其它同H组处理。再灌注结束后抽血测超氧化物歧化酶(SOD)、丙二醛(MDA)含量,测心梗面积,免疫印迹法测心肌S-腺苷蛋氨酸合成酶(S-adenosylmethionine synthetase,SAM-s)的表达。
结果:心肌梗死面积与IR组(38.27%±5.64%)比,H组(25.40%±3.54%)减小(P<0.05),D组(40.53%±5.24%)无明显差异(P>0.05)。心肌SAM-s表达与S组比,IR组、H组和D组均升高(P<0.05);与IR组比,H组降低(P<0.05),D组无明显差异(P>0.05)。与IR组比,H组血清SOD的活性含量增高、MDA的降低(P<0.05),D组无明显差异(P>0.05)。
结论:硫化氢预处理对大鼠心肌具有保护作用,其机制可能是通过抑制心肌SAM-s表达,减少氧自由基的生成,增强心肌抗氧化能力有关。
关键词: 硫化氢/药理学; 甲硫氨酸腺苷转移酶; 心肌再灌注损伤/药物疗法; 心肌再灌注损伤/病理学; 肌细胞心脏/药物作用; 肌细胞心脏/病理学    
Abstract: Objective: To investigate the effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats.
Methods: Sprague-Dawley male rats were divided into 4 groups with 10 in each group:in S group rats received sham operation; in IR group rats were given with NS(1.0 ml/kg iv) 24 h before ischemia; in H group rats were treated with NaHS(0.05 mg/kg iv) 24 h before ischemia; and in D group,NaHS-treated rats received 5-hydroxydecanoate(5-HD) 15 min before ischemia. Rats in IR group,H group and D group were subjected to ischemia by occlusion of coronary artery for 30 min followed by 2 h of reperfusion.At the end of the reperfusion,myocardial infarct size was measured.SAM-s was measured by Western blotting.Plasma SOD activity and MDA were determined at the end of reperfusion.
Results: The infarct size was significantly lesser in H group (25.40%± 3.54%) than that in IR group (38.27%±5.64%,P<0.05).The SAM-s protein expression in myocardium was significantly lower in H group than that in IR group.The plasma MDA content was significantly lower and SOD activity was higher in H group than those in IR group,but there was no difference between IR group and D group.
Conclusion: The hydrogen sulfide preconditioning attenuates myocardial IR injury possibly through down-regulating SAM-s expression,reducing the production of oxygen free radicals and enhancing anti-oxidize effect in rats.
Key words: Hydrogen sulfide/pharmacology    Methionine adenosyltransferase; Myocardial reperfusion injury/drug therapy; Myocardial reperfusion injury/pathology; Myocytes    cardiac/drug effects; Myocytes    cardiac/pathology
出版日期: 2012-09-25
服务  
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章  

引用本文:

李辉;冉珂;唐正国;李双凤;常业恬. 硫化氢预处理对大鼠心肌缺血再灌注损伤的保护作用[J]. 浙江大学学报(医学版), 2012, 41(5): 559-563.

链接本文:

https://www.zjujournals.com/med/CN/Y2012/V41/I5/559

[1] 赵誉,张凤,赵筱萍,袁玮,张金华,王毅. 参麦注射液保护氧化损伤心肌细胞线粒体的机制研究[J]. 浙江大学学报(医学版), 2018, 47(5): 507-513.
[2] 泮辉,等. 胎盘生长因子恢复急性心肌梗死大鼠心功能及其机制[J]. 浙江大学学报(医学版), 2014, 43(4): 441-447.