Please wait a minute...
浙江大学学报（医学版）
浙江大学学报（医学版）  2012, Vol. 41 Issue (5): 559-563    DOI: 10.3785/j.issn.1008-9292.2012.05.015
论著     
硫化氢预处理对大鼠心肌缺血再灌注损伤的保护作用
李辉1，冉珂1，唐正国2，李双凤1，常业恬1
1.中南大学湘雅二医院麻醉科，湖南 长沙 410011； 2.长沙市第三医院麻醉科，湖南 长沙 410015
Effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats
LI Hui1，RAN Ke1，TANG Zheng-guo2，LI Shuang-feng1，CHANG Ye-tian1
1.Department of Anesthesiology，Second Xiangya Hospital，Central South University，Changsha 410011，China; 2.Department of Anesthesiology，the Third Changsha Municipal Hospital，Changsha 410015，China
 全文: PDF(1038 KB)   HTML ( ) 
摘要： 目的：探讨硫化氢预处理对大鼠心肌缺血再灌注损伤的保护作用。
方法：健康成年SD雄性大鼠40只，随机分成4组,每组10只。假手术组(S组)仅开胸并分离冠状动脉左前降支，不阻断血流150 min；缺血再灌注组(IR组)行冠状动脉左前降支阻断30 min，再灌注120 min；硫化氢预处理组(H组)予以静脉注射硫化氢钠0.05 mg/kg，给药后24 h同IR组处理；硫化氢预处理+线粒体KATP通道阻断剂（5-羟葵酸，5-HD）组(D组)缺血前15 min静脉注射5-HD 5 mg/kg，其它同H组处理。再灌注结束后抽血测超氧化物歧化酶(SOD)、丙二醛(MDA)含量，测心梗面积，免疫印迹法测心肌S-腺苷蛋氨酸合成酶（S-adenosylmethionine synthetase，SAM-s）的表达。
结果：心肌梗死面积与IR组(38.27%±5.64%)比，H组(25.40%±3.54%)减小（P<0.05），D组(40.53%±5.24%)无明显差异（P＞0.05）。心肌SAM-s表达与S组比，IR组、H组和D组均升高（P<0.05）；与IR组比，H组降低（P<0.05），D组无明显差异（P＞0.05）。与IR组比，H组血清SOD的活性含量增高、MDA的降低(P<0.05)，D组无明显差异（P＞0.05）。
结论：硫化氢预处理对大鼠心肌具有保护作用，其机制可能是通过抑制心肌SAM-s表达，减少氧自由基的生成，增强心肌抗氧化能力有关。
关键词： 硫化氢/药理学； 甲硫氨酸腺苷转移酶； 心肌再灌注损伤/药物疗法； 心肌再灌注损伤/病理学； 肌细胞;  心脏/药物作用； 肌细胞;  心脏/病理学    
Abstract: Objective： To investigate the effects of hydrogen sulfide preconditioning on myocardial ischemia reperfusion injury in rats.
Methods： Sprague-Dawley male rats were divided into 4 groups with 10 in each group:in S group rats received sham operation; in IR group rats were given with NS(1.0 ml/kg iv) 24 h before ischemia; in H group rats were treated with NaHS(0.05 mg/kg iv) 24 h before ischemia; and in D group，NaHS-treated rats received 5-hydroxydecanoate(5-HD) 15 min before ischemia. Rats in IR group，H group and D group were subjected to ischemia by occlusion of coronary artery for 30 min followed by 2 h of reperfusion.At the end of the reperfusion，myocardial infarct size was measured.SAM-s was measured by Western blotting.Plasma SOD activity and MDA were determined at the end of reperfusion.
Results： The infarct size was significantly lesser in H group (25.40%± 3.54%) than that in IR group (38.27%±5.64%，P<0.05).The SAM-s protein expression in myocardium was significantly lower in H group than that in IR group.The plasma MDA content was significantly lower and SOD activity was higher in H group than those in IR group，but there was no difference between IR group and D group.
Conclusion： The hydrogen sulfide preconditioning attenuates myocardial IR injury possibly through down-regulating SAM-s expression，reducing the production of oxygen free radicals and enhancing anti-oxidize effect in rats.
Key words: Hydrogen sulfide/pharmacology    Methionine adenosyltransferase； Myocardial reperfusion injury/drug therapy； Myocardial reperfusion injury/pathology； Myocytes    cardiac/drug effects； Myocytes    cardiac/pathology
出版日期: 2012-09-25
服务　　
把本文推荐给朋友
加入引用管理器
E-mail Alert
RSS
作者相关文章　　

引用本文:

李辉;冉珂;唐正国;李双凤;常业恬. 硫化氢预处理对大鼠心肌缺血再灌注损伤的保护作用[J]. 浙江大学学报（医学版）, 2012, 41(5): 559-563.

链接本文:

https://www.zjujournals.com/med/CN/Y2012/V41/I5/559

[1] 赵誉,张凤,赵筱萍,袁玮,张金华,王毅. 参麦注射液保护氧化损伤心肌细胞线粒体的机制研究[J]. 浙江大学学报（医学版）, 2018, 47(5): 507-513.
[2] 泮辉，等. 胎盘生长因子恢复急性心肌梗死大鼠心功能及其机制[J]. 浙江大学学报（医学版）, 2014, 43(4): 441-447.