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浙江大学学报(医学版)  2012, Vol. 41 Issue (4): 434-440    DOI: 10.3785/j.issn.1008-9292.2012.04.013
论著     
β-聚苹果酸高产菌株的筛选及鉴定
徐玲芬,陈忠华,赵婧,阮红,刘森泉
浙江大学城市学院医学与生命科学学院,浙江 杭州 310015
Screening and identification of high-yield poly(β-malic acid) bacterial strain
XU Ling-fen, CHENG Zhong-hua, ZHAO Jing , RUAN Hong, LIU Sen-quan
School of Medicine and Life Science, Zhejiang University City College, Hangzhou 310015, China
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摘要: 目的:从自然界筛选β-聚苹果酸(PMLA)高产菌株,并对其进行菌种鉴定。
方法:从自然界取样,经过单菌落分离,根据菌落形态特征、聚苹果酸的定性检测和核酸序列分类鉴定手段,筛选PMLA高产菌株。
结果:分离得到一株PMLA高产菌株,编号为Ⅱ04,经形态学研究和ITS序列分析,确定为短梗霉菌Aureobasidium pullulans,命名为Aureobasidium pullulans ZUCC-41。该菌在25℃,220 r/min振荡培养7 d,采用高效液相色谱法(HPLC)进行产物定量分析,检测到PMLA产量可达26.23 g/L。
结论:从自然界中分离得到一株PMLA高产菌株,经鉴定为短梗霉菌Aureobasidium pullulans,其发酵产量可达26.23 g/L。
关键词: 苹果酸盐 有丝分裂孢子真菌/分离和提纯 聚苹果酸高产菌株鉴定    
Abstract: Objective: To isolate and identify the high-yield poly-malic acid (PMLA) bacterial strains from the nature.
Methods: Samples were collected and cultured. The high-yield PMLA bacterial strains were screened through morphological observation, qualitative PMLA tests by HPLC and ITS sequence analysis on the isolated bacterial strains.
Results: A high-yield PMLA strainⅡ04 was isolated, the yield of PMLA of the strain reached to 26.23g/L in the rotary shaker at 25℃ for 7d. From morphological observation and ITS sequences analysis, the strain belonged to Aureobasidium pullulans, and named as Aureobasidium pullulans ZUCC-41. Conclusion: A high-yield bacterial strain has been isolated from the nature and identified to be Aureobasidium pullulans.
Key words: Malates    Mitosporic fungi/isol    Poly(malic acid)    High-Producing Strain; Identification
出版日期: 2012-07-25
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徐玲芬;陈忠华;赵婧;阮红;刘森泉. β-聚苹果酸高产菌株的筛选及鉴定[J]. 浙江大学学报(医学版), 2012, 41(4): 434-440.

链接本文:

https://www.zjujournals.com/med/CN/Y2012/V41/I4/434

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