Objective: To determine Th1/Th2 cytokines and chemokines in patients with allergic diseases and its clinic significance. Methods: Serum levels of IFN-γ,IL-4,IL-5,IL-13,Eotaxin,RANTES and LTB4 were determined from peripheral blood of 64 allergic patients and 21 healthy controls with ELISA. Results: IL-4,IL-5,IL-13 and Eotaxin,LTB4 were increased significantly in serum of allergic patients compared with those of controls (P<0.05).There were no significant differences in serum levels of IFN-γ and RANTES between patients and controls (P>0.05).Serum levels of IL-4,IL-5,IL-13 and LTB4 were correlated with each other(P<0.01).Eotaxin,RANTES and IFN-γ levels were also significantly correlated with each other(P<0.05).LTB4 was correlated with Eotaxin as well(P<0.01). Conclusion: A wide range of cytokines and chemokines is involved in allergic diseases,which may play their roles in a complex interactive manner.