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浙江大学学报(医学版)  1999, Vol. 28 Issue (6): 260-261    DOI: 10.3785/j.issn.1008-9292.1999.06.006
论著     
视频显示终端脉冲磁场对细胞间隙连接通讯功能的影响研究
付一提, 姜槐, 鲁德强, 曾群力
浙江大学医学院, 浙江 杭州 310031
Study on the Effects of VDT Pulse Magnetic Field on Gap Junctional Intercellular Communication
FU Yi-ti, CHIANG Huai, LU De-qiang
Medical College, Zhejiang University, Hangzhou, 310031
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摘要:

目的:研究视频显示终端(VDT)脉冲磁场对细胞间隙连接通讯功能的影响。方法:用15.6 kHz、峰值强度为200 μT的脉冲磁场(PMF)和(或)十四酰基咐拜醇酯(TPA:5 ng/ml)对培养的中国仓鼠肺成纤维细胞(CHL)进行辐射24 h,采用离子电渗注射法观察荧光黄向与其接触的周围细胞的传递情况。结果:TPA对细胞间隙连接通讯(GJIC)功能具有抑制作用,与空白对照组相比有显著差异(P<0.01),单纯VDT组对GJIC无抑制作用(P>0.05),亦未见该脉冲磁场对TPA的抑制作用有增强效应。结论:视频显示终端脉冲磁场(15.6 kHz)对细胞间隙连接通讯功能无直接和(或)协同TPA的抑制作用。

关键词: 视频显示终端脉冲磁场细胞间接合部信号传递    
Abstract:

Objective:To study the effects of VDT pulse magnetic field on gap junctional intercellular communication.Methods:CHL cells were treated 24 h with 15.6 kHz pulse magnetic field at 200 μT peak to peak and (or) TPA (5 ng/ml).Lucifer dye was led into CHL cell by iontophoretic injection and counted the number of dye coupled cells around the cell injected.Results:TPA significantly suppressed GJIC compared with control group (P<0.01),but VDT pulse magnetic field had no suppression compared with control group (P>0.05).Conclusion:VDT pulse magnetic field alone or with TPA had no effects.

Key words: Video display terminal    Pulse magnetic field    intercellular junctions    signal transduction
收稿日期: 1999-01-12 出版日期: 1999-11-25
:  R329.2+5  
作者简介: 付一提(1954-),女,实验师,从事电磁波生物学效应研究.
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引用本文:

付一提, 姜槐, 鲁德强, 曾群力. 视频显示终端脉冲磁场对细胞间隙连接通讯功能的影响研究[J]. 浙江大学学报(医学版), 1999, 28(6): 260-261.

FU Yi-ti, CHIANG Huai, LU De-qiang. Study on the Effects of VDT Pulse Magnetic Field on Gap Junctional Intercellular Communication. Journal of ZheJiang University(Medical Science), 1999, 28(6): 260-261.

链接本文:

https://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.1999.06.006        https://www.zjujournals.com/med/CN/Y1999/V28/I6/260

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