Objective:To develop monoclonal antibodies against Neisseria gonorrhoeae for establishing a new diagnostic method of gonorrhoea.Methods:Monoclonal antibodies against strain 29106-5 of N.gonorrhoeae were prepared by the lymphocyte hybridoma technique.The specificity and sensitivity of McAbs were studied using the sandwich method of ELISA with double antibodies.Results:Five cell lines of hybridoma secreting constantly McAbs to N.gonorrhoeae were obtained.The titer of McAbs reached to 1:8192~1:32786.All McAbs could react to N.gonorrhoeae,but failed to react to other microbe such as N.Meningitidis,staphylococcus and E.Coli.The minimum dose of N.gonorrhoeae which can be detected by ELISA was 1×104 bacteria per ml.Conclusion:The sandwich method of ELISA using McAbs to detect N.gonorrhoeae possessed higher specificity and sensitivity.
沈建根, 鲍建芳, 邵传森, 董玉娥. 抗淋球菌单克隆抗体的制备及其生物学特性研究[J]. 浙江大学学报（医学版）, 1999, 28(2): 49-50.
Shen Jiangen, Bao Jianfang, Shao Chuansen. Preparation of Monoclonal Antibody Against Neisseria Gonorrhoeae and Studies on Its Biological Characteristics. Journal of ZheJiang University(Medical Science), 1999, 28(2): 49-50.