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浙江大学学报（医学版）
浙江大学学报（医学版）  1998, Vol. 27 Issue (2): 52-55    DOI: 10.3785/j.issn.1008-9292.1998.02.002
论著     
抗人精子外套抗原单抗的纯化及相应抗原的提纯和鉴定
黄铭燕, 刘子贻, 朱寿民
浙江医科大学基础医学院生物化学教研室
The Purification of Monoclonal Antibody to Human Sperm-Coating Antigens and the Separation and Identification of the Related Antigen
Huang Mingyan, Liu Ziyi, Zhu Shoumin
Department of biochemistry, Zhejiang Medical University
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摘要： 目的:研究人类精浆中一类特异性的抗原成份(精子外套抗原sperm-coating antigens,SCA)。方法:本实验用已制备得的一株抗人精浆来源的精子外套抗原单克隆杂交瘤细胞株D1,在小鼠腹腔增殖生产大量腹水单克隆抗体,通过快速蛋白液相层析仪(FPLC)系统的羟基磷灰石柱,一次性从小鼠腹水中纯化此抗体,并制备免疫亲和层析柱,从精浆中分离到相应的抗原成份。结果:经鉴定该抗原成份分子量为20kD,来源于精囊腺,具有器官特异性。结论:该SCA的提纯,为抗精子避孕疫苗的研制提供了基础实验材料,并且有助于进一步研究其在生殖活动和免疫不孕病因中的作用。
关键词： 精子外套抗原/分离和提纯;  人精浆抗原/分离和提纯;  IgM单克隆抗体/分离和提纯;  精子/免疫学;  精液/免疫学    
Abstract: Objective: To investigate the sperm-coating antigens (SCA) in human seminal plasma (HSP). Methods: Monoclonal antibody (McAb IgM) to HSP-specific antigen was prepared by using a hybrid cell line D1 and purified with hydroxyapatite (HA) on Fast Protein Liquid Chromatography (FPLC) system with one step and a McAb-bound Sepharose 4B column was prepared. The related HSP-antigen to McAb was obtained by immune affinity chromatography. Results: The purified antigen mainly gave a single band on SDS-PAGE in a position corresponding to a molecular weight of 20 kD, and was proved to be a HSP-specific antigen. Conclusion: This purified SCA may be used for preparation of anti-sperm immunocontraceptive vaccines, and it can also be used to study reproduction and immunological sterility.
Key words: Sperm-coating antigen/isol    Huma seminal plasma antigen/isol    IgM: Antibodies, monoclonal/isol    Spermatozoa/immunol    Semen/immunol
收稿日期: 1997-08-27 出版日期: 1998-03-25
基金资助: 浙江省自然科学基金
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黄铭燕
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引用本文:

黄铭燕, 刘子贻, 朱寿民. 抗人精子外套抗原单抗的纯化及相应抗原的提纯和鉴定[J]. 浙江大学学报（医学版）, 1998, 27(2): 52-55.

Huang Mingyan, Liu Ziyi, Zhu Shoumin. The Purification of Monoclonal Antibody to Human Sperm-Coating Antigens and the Separation and Identification of the Related Antigen. Journal of ZheJiang University(Medical Science), 1998, 27(2): 52-55.

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https://www.zjujournals.com/med/CN/10.3785/j.issn.1008-9292.1998.02.002    或    https://www.zjujournals.com/med/CN/Y1998/V27/I2/52

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