本文以幽门螺旋菌(HP)反复冻融后的可溶性成分作包被抗原, 用酶联免疫吸附试验(ELISA)对经胃镜检查的76例慢性胃炎患者、62例1~7岁体检儿童、41例18~25岁正常成人进行了血清HP IgG的测定。结果显示, 患者组抗体检出率为75.0%、与HP培养法相比, 符合率为88.16%, 诊断敏感性为91.38%、特异性为77.78%、阳性预测值为92.98%、阴性预测值为73.68%。若以1~7岁儿童作为未感染HP者, 则本法诊断特异性为80.61%。不同人群抗体阳性率以患者组最高、正常成人组次之、儿童组最低, 各组间的抗体水平有显著性差异。研究表明, 本法可用于临床HP感染的初筛检查及正常人群的流行病学调查。
The ELISA system with an antigen disrupted from campylobacter pylori (CP) by freezing and thawing has been used to detect serum anti-CP IgG antibody in 76 patients with chronic gastric diseases, 62 healthy children (aged 1~7) and 41 normal adults (aged 18~25).The results showed a 75% positive rate in the patient group.As compared with CP culture, the coincident rate was 88.16%.The sensitivity, specificity, positive and negative predictive values were 91.38%, 77.78%, 92.98% and 73.68%, respectively.If the 1~7 year-old children are considered as the CP noninfected group, the specificity of this serologic test should be 80.61%.It has been found that the patient group had the highest positive rate of anti-CP IgG and there was significant difference in the level of serum CP antibody among various population groups.Our study indicated that this serologic test could be used to screen patients prior to endoscopy and in massive epidemiological research as well.
方平楚, 兰茂功, 吴清明, 尹秀, 姚碧光, 黄怀德, 王蔚芳. 酶联免疫吸附法检测幽门螺旋菌抗体IgG的研究及其临床意义[J]. 浙江大学学报（医学版）, 1991, 20(6): 255-258.
Fang Pingchu, Lan Maogong, et al, Yao Biguang, Huang Huade, et al. STUDY ON THE DETECTION OF IgG ANTIBODY AGAINST CAMPYLOBACTER PYLORI BY ELISA AND ITS CLINICAL SIGNIFICANCE. Journal of ZheJiang University(Medical Science), 1991, 20(6): 255-258.