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浙江大学学报(医学版)  2022, Vol. 51 Issue (4): 397-404    DOI: 10.3724/zdxbyxb-2022-0158
专题报道     
高原低氧环境下红景天苷对小鼠运动耐力的影响
秦宁宁1,2,谢华1,赵安鹏1,2,张晓静1,孙月梅1,李文斌1,*,王荣1,2,*
1. 中国人民解放军联勤保障部队第九四〇医院药剂科 全军高原医学实验室,甘肃 兰州 730050
2. 兰州大学药学院,甘肃 兰州 730000
Effects of salidroside on exercise tolerance of mice under high altitude hypoxia environment
QIN Ningning1,2,XIE Hua1,ZHAO Anpeng1,2,ZHANG Xiaojing1,SUN Yuemei1,LI Wenbin1,*,WANG Rong1,2,*
1. Department of Pharmacy, the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army, Key Laboratory of the Plateau Medicine, Lanzhou 730050, China;
2. School of Pharmacy, Lanzhou University, Lanzhou 730000, China
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摘要:

目的:探讨高原低氧环境下红景天苷对小鼠运动耐力的影响。 方法:C57BL/6J健康雄性小鼠随机分为常氧对照组、模型对照组、红景天胶囊组及红景天苷小剂量 (5?mg/kg)、中剂量 (10?mg/kg)、大剂量 (20?mg/kg), 每组15只。除外常氧对照组外,各组按剂量预防给药 3?d后急进海拔 4010?m的高原,缺氧暴露 1?d后采用力竭游泳实验测定小鼠力竭时间;苏木精-伊红染色观察肝及肌肉组织的病理学变化;检测并比较各组丙二醛(MDA)、过氧化氢(H 2O 2)、谷胱甘肽(GSH)、总超氧化物歧化酶(T-SOD)、糖原、乳酸、腺苷三磷酸(ATP)酶活性水平。 结果:与常氧对照组比较,模型对照组力竭游泳时间缩短( P<0.05),肝及肌肉组织出现病理损伤、氧化应激水平显著提高、钠钾ATP酶和钙镁ATP酶活性升高、糖原含量减少;与模型对照组比较,红景天胶囊组和红景天苷各剂量组力竭游泳时间显著延长(均P<0.05),肝、肌肉组织的病理损伤减轻,且肝、肌肉组织中MDA、H2O 2、乳酸含量不同程度降低,GSH、肝糖原和肌糖原含量增加,T-SOD、ATP酶活性增强(均 P<0.05)。结论:红景天苷具有显著的抗疲劳活性,其抗疲劳作用与减少氧化应激损伤、降低不良代谢产物的堆积和增加能量物质的储备相关。

关键词: 低压性低氧红景天苷抗疲劳力竭游泳氧化应激能量代谢小鼠    
Abstract:

Objective: To investigate the effect of salidroside on the exercise tolerance of mice under high altitude hypoxia environment. Methods: C57BL/6J healthy male mice were randomly divided into normoxia control group, model control group, Rhodiola rosea capsule group and salidroside low-dose (5?mg/kg), medium-dose (10?mg/kg) and high-dose (20?mg/kg) groups, with 15 mice in each group. After 3 days, all groups (except the normoxia control group) entered a plateau with an altitude of 4010?m. After 1?day of hypoxia exposure, the exhausted swimming test was performed to determine the exhaustive time of mice; the pathological changes of liver and muscle tissue were observed with hematoxylin and eosin staining. The levels of malondialdehyde (MDA), hydrogen peroxide (H 2O 2), glutathione (GSH), total superoxide dismutase (T-SOD), glycogen, lactate and ATPase were measured and compared among groups. Results: Compared with the normoxia control group, the exhaustive swimming time of the model control group was shortened ( P<0.05), the liver tissue and muscle tissue were pathologically damaged, the level of oxidative stress was significantly increased, the levels of sodium potassium ATPase and calcium magnesium ATPase were significantly increased. Compared with the model control group, the exhaustive swimming time of the mice in theRhodiola rosea capsule group and salidroside groups was significantly prolonged ( P<0.05). The oxidative stress injury was alleviated, the contents of MDA, H2O 2 and lactic acid in liver and muscle tissues decreased, the contents of GSH, liver glycogen and muscle glycogen increased, and the activities of T-SOD and ATPase increased (all P<0.05).Conclusion: Salidroside has significant anti-fatigue activity, and its anti-fatigue effect is related to the reduction of oxidative stress damage, the reduction of the accumulation of undesirable metabolites and the increase in the reserve of energy substances.

Key words: Hypobartic hypoxia    Salidroside    Anti-fatigue    Exhausted swimming    Oxidative stress    Energy metabolism    Mouse
收稿日期: 2022-04-14 出版日期: 2022-11-16
CLC:  R96  
基金资助: 国家自然科学基金(82173738); 军队后勤科研项目(CLB19J049)
通讯作者: 李文斌,王荣   
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引用本文:

秦宁宁,谢华,赵安鹏,张晓静,孙月梅,李文斌,王荣. 高原低氧环境下红景天苷对小鼠运动耐力的影响[J]. 浙江大学学报(医学版), 2022, 51(4): 397-404.

QIN Ningning,XIE Hua,ZHAO Anpeng,ZHANG Xiaojing,SUN Yuemei,LI Wenbin,WANG Rong. Effects of salidroside on exercise tolerance of mice under high altitude hypoxia environment. J Zhejiang Univ (Med Sci), 2022, 51(4): 397-404.

链接本文:

https://www.zjujournals.com/med/CN/10.3724/zdxbyxb-2022-0158        https://www.zjujournals.com/med/CN/Y2022/V51/I4/397

图1  各组模型鼠肝组织病理学变化(苏木精-伊红染色) A:常氧对照组;B:模型对照组;C:红景天胶囊组;D:红景天苷小剂量组;E:红景天苷中剂量组;F:红景天苷大剂量组. 常氧对照组肝组织正常无损伤,模型对照组出现少量坏死和水肿现象,红景天胶囊和红景天苷各剂量组坏死和水肿现象均较模型对照组减轻. 标尺 =50 μm.
图2  各组模型鼠肌肉组织病理学变化(苏木精-伊红染色) A:常氧对照组;B:模型对照组;C:红景天胶囊组;D:红景天苷小剂量组;E:红景天苷中剂量组;F:红景天苷大剂量组. 常氧对照组肌纤维整齐完整,模型对照组肌纤维紊乱变形现象,红景天胶囊和红景天苷各剂量组肌纤维紊乱变形现象均较高原缺氧组减轻. 标尺 =50 μm.

组 别

n

MDA(nmol/mg蛋白)

H 2O 2(nmol/g蛋白)

GSH(mg/g蛋白)

T-SOD(U/mg蛋白)

常氧对照组

8

0.54±0.04

6.25±0.58

7.18±1.76

318.89±14.40

模型对照组

8

0.77±0.15 **

16.05±3.65 **

5.41±2.20 *

280.39±16.09 **

红景天胶囊组

8

0.64±0.06 #

11.89±1.53 #

5.76±1.20

295.07±19.08

红景天苷小剂量组

8

0.61±0.11 #

13.87±1.23

6.11±0.84

288.42±27.97

红景天苷中剂量组

8

0.60±0.07 #

11.64±1.07 #

7.03±0.99 #△

301.15±8.33 ##

红景天苷大剂量组

8

0.65±0.05 #

10.65±1.63 ##

7.16±1.59 #

286.64±37.34

表1  各组肝组织中氧化应激指标检测结果比较

组 别

n

MDA(nmol/mg蛋白)

H 2O 2(nmol/g蛋白)

GSH(mg/g蛋白)

T-SOD(U/mg蛋白)

常氧对照组

8

3.71±0.31

7.73±1.19

2.58±0.17

93.52±5.62

模型对照组

8

3.65±0.21

7.67±1.65

1.54±0.15 **

93.72±8.29

红景天胶囊组

8

3.32±0.35 #

6.41±0.75

2.28±0.25 ##

101.17±6.00 #

红景天苷小剂量组

8

3.75±0.34

6.05±0.30 #

1.82±0.14 ##△△

96.57±9.39

红景天苷中剂量组

8

3.72±0.30

6.06±0.53 #

1.99±0.20 ##△

98.54±4.11

红景天苷大剂量组

8

3.01±0.30 ##

6.19±0.72 #

2.12±0.14 ##

102.14±6.17 #

表2  各组肌肉组织中氧化应激指标检测结果比较

组 别

n

乳酸

(mmol/g蛋白)

钙镁ATP酶

(μmolPi·gHb –1·h –1)

钠钾ATP酶

(μmolPi·gHb –1·h –1)

糖原(mg/g组织)

常氧对照组

8

0.32±0.01

0.68±0.08

0.86±0.07

12.77±3.22

模型对照组

8

0.34±0.02 *

0.82±0.12 *

1.08±0.09 **

1.56±0.43 **

红景天胶囊组

8

0.31±0.02 #

1.04±0.12 ##

1.21±0.08 ##

2.07±0.31 #

红景天苷小剂量组

8

0.35±0.03

1.04±0.07 ##

1.22±0.08 ##

1.98±0.20 #

红景天苷中剂量组

8

0.30±0.02 ##

1.03±0.14 ##

1.13±0.10

2.58±1.05 #

红景天苷大剂量组

8

0.31±0.03 #

0.97±0.14 #

1.12±0.07

2.30±0.77 #

表3  各组肝组织中能量代谢相关指标检测结果比较

组 别

n

乳酸

(mmol/g蛋白)

钙镁ATP酶

(μmolPi·gHb –1·h –1)

钠钾ATP酶

(μmolPi·gHb –1·h –1)

糖原(mg/g组织)

常氧对照组

8

1.29±0.11

8.21±2.47

3.29±1.09

0.61±0.11

模型对照组

8

1.39±0.11

9.73±0.88

2.25±0.27 *

0.38±0.06 **

红景天胶囊组

8

1.28±0.20

10.86±0.78 #

1.84±0.31 #

0.50±0.10 ##

红景天苷小剂量组

8

1.27±0.05 #

9.58±1.22

2.43±1.09

0.39±0.06

红景天苷中剂量组

8

1.27±0.10 #

11.12±1.49 #

2.50±0.54

0.42±0.06

红景天苷大剂量组

8

1.22±0.11 ##

12.25±0.78 ##△△

2.49±0.39 △△

0.40±0.04

表4  各组肌肉组织中能量代谢相关指标检测结果比较
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