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浙江大学学报（医学版）
浙江大学学报（医学版）  2021, Vol. 50 Issue (5): 575-581    DOI: 10.3724/zdxbyxb-2021-0319
专题报道     
7-羟乙基白杨素对低压性缺氧大鼠运动性疲劳具有保护作用
苗路伟1,2,赵彤1,2,高迎春2,景临林2,黄琼3,*(),马慧萍1,2,*()
1.甘肃中医药大学药学院，甘肃 兰州 730000
2.中国人民解放军联勤保障部队第九四〇医院药剂科 全军高原医学实验室，甘肃兰州 730050
3.中国人民解放军联勤保障部队第九四〇医院医疗保障中心，甘肃 兰州 730050
Protective effects of 7-hydroxyethyl chrysin on rats with exercise-induced fatigue in hypobaric hypoxia environment
MIAO Luwei1,2,ZHAO Tong1,2,GAO Yingchun2,JING Linlin2,HUANG Qiong3,*(),MA Huiping1,2,*()
1. College of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China;
2. Key Laboratory of the Plateau Medicine, the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army, Lanzhou 730050, China;
3. Medical Support Center, the 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army, Lanzhou 730050, China
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摘要：

目的：探究7-羟乙基白杨素（7-HEC）对低压性缺氧大鼠运动性疲劳的保护作用。方法：选取40只健康雄性Wistar大鼠随机分为常氧对照组、缺氧对照组、白杨素组、7-HEC组，每组各10只，白杨素组和7-HEC组分别给予白杨素和7-HEC 350?mg/kg，常氧对照组和缺氧对照组给予等量灭菌注射用水。常氧对照组饲养于当地海拔；其余三组置于低压性缺氧动物实验舱模拟7000?m海拔进行缺氧处理。连续灌胃给药3?d后进行力竭实验，统计各组游泳时间。力竭实验后立即采集大鼠的血液、肝组织和骨骼肌组织，观察肝组织和骨骼肌组织病理学变化，测定血尿素氮、乳酸、丙二醛、总超氧化物歧化酶（T-SOD）、肌肝糖原含量等指标。结果：与缺氧对照组比较，7-HEC组负重游泳时间延长[（2.58±0.77）和（4.04±1.30）min，P<0.01]；肝组织和骨骼肌组织的病理学变化改善；血尿素氮[（3.24±0.93）和（2.22±0.60）mmol·L–1·min–1]、乳酸[肝组织为（0.14±0.05）和（0.10±0.03）mg·g–1·min–1，骨骼肌组织为（0.26±0.06）和（0.18±0.07）mg·g–1·min–1]、丙二醛[肝组织为（1.37±0.48）和（0.78±0.28）nmol·mg–1·min–1，骨骼肌组织为（0.87±0.19）和（0.63±0.11）nmol·mg–1·min–1]生成速率减小（均P<0.05）；糖原[肝组织为（15.16±2.69）和（18.89±2.88）mg/g，骨骼肌组织为（1.46±0.49）和（1.78±0.48）mg/g]和T-SOD[肝组织为（1.87±0.01）和（2.68±0.12）U/mL，骨骼肌组织为（5.46±0.42）和（6.52±0.96）U/mL]增加（均P<0.05）。结论：7-HEC对低压性缺氧大鼠运动性疲劳具有明显保护作用。
关键词： 7-羟乙基白杨素;  抗疲劳;  低压性缺氧;  力竭实验;  大鼠    
Abstract:

Objective: To investigate the protective effect of 7-hydroxyethyl chrysin (7-HEC) on rats with exercise-induced fatigue in hypobaric hypoxic condition.Methods:Forty healthy male Wistar rats were randomly divided into four groups with 10 rats in each group: control group, model group, chrysin group and 7-HEC group. The rats in control group were raised at local altitude but other three groups were raised in a simulating altitude of 7000?m for hypobaric hypoxia treatment. The chrysin group and 7-HEC group were given 350?mg/kg chrysin or 7-HEC by gavage for 3?d, respectively; while the control group and model group were given the same amount of sterilized water. The weight-bearing swimming tests were performed 3?d later, and the weight-bearing swimming time was documented. After rats were sacrificed, the liver and skeletal muscle tissue samples were taken for pathological examination and determination of lactate, malondialdehyde (MDA), total superoxide dismutase (T-SOD) and glycogen levels. Blood urea nitrogen was also determined. Results:Compared with the model group, weight-bearing swimming times were significantly prolonged in 7-HEC group [(2.58±0.77) vs. (4.04±1.30)?min, P<0.01]; pathological changes in liver and skeletal muscle tissue were attenuated; generation rate of blood urea nitrogen [(3.24±0.93) vs. (2.22±0.60) mmol·L–1·min–1, P<0.05], lactate [liver: (0.14±0.05) vs. (0.10±0.03)?mg·g–1·min–1, skeletal muscle: (0.26±0.06) vs. (0.18±0.07) mg·g–1·min–1] and MDA [liver: (1.37±0.48) vs. (0.78±0.28)?nmol·mg–1·min–1, skeletal muscle: (0.87±0.19) vs. (0.63±0.11)?nmol·mg–1·min–1] were significantly reduced (all P< 0.05); glycogen content [liver: (15.16±2.69) vs. (18.89±2.88)?mg/g, skeletal muscle: (1.46±0.49) vs.(1.78±0.48)?mg/g] and T-SOD [liver: (1.87±0.01) vs. (2.68±0.12) U/mL, skeletal muscle: (5.46±0.42) vs. (6.52±0.96) U/mL] were significantly improved (allP<0.05).Conclusion:7-HEC has significant protective effect on the rats with exercise-induced fatigue in hypobaric hypoxia condition.
Key words: 7-hydroxyethylchrysin    Anti-fatigue    Hypobaric hypoxia    Exhaustive exercise    Rats
收稿日期: 2021-02-05 出版日期: 2021-12-29
:  R965.1  
基金资助: 国家自然科学基金（81571847，81872796）； 军队后勤科研计划（CWH17J010，CLB18J028）
通讯作者: 黄琼,马慧萍     E-mail: mahuiping2020@163.com
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引用本文:

苗路伟,赵彤,高迎春,景临林,黄琼,马慧萍. 7-羟乙基白杨素对低压性缺氧大鼠运动性疲劳具有保护作用[J]. 浙江大学学报（医学版）, 2021, 50(5): 575-581.

MIAO Luwei,ZHAO Tong,GAO Yingchun,JING Linlin,HUANG Qiong,MA Huiping. Protective effects of 7-hydroxyethyl chrysin on rats with exercise-induced fatigue in hypobaric hypoxia environment. J Zhejiang Univ (Med Sci), 2021, 50(5): 575-581.

链接本文:

https://www.zjujournals.com/med/CN/10.3724/zdxbyxb-2021-0319    或    https://www.zjujournals.com/med/CN/Y2021/V50/I5/575

图 1  白杨素和7-羟乙基白杨素的化学结构式
图 2  大鼠肝组织和骨骼肌组织病理学变化（HE染色）常氧对照组肝组织除细胞间隙增大外未见明显损伤；缺氧对照组肝组织细胞排列散乱，出现大量圆形空泡，且炎症细胞浸润明显；白杨素组和7-HEC组肝组织细胞排列趋于正常，炎症细胞浸润减少，7-HEC组减少更明显. 常氧对照组骨骼肌结构完整，未见明显损伤；缺氧对照组肌纤维明显断裂、肿胀，炎症细胞浸润明显，肌束界限模糊；白杨素组和7-HEC组肌纤维排列整齐，炎症细胞显著减少，骨骼肌细胞趋于正常，且以7-HEC组更明显.7-HEC：7-羟乙基白杨素. 标尺=200 μm.

组别

n

糖原含量（mg/g）

乳酸生成速率（mg·g–1·min–1）

肝组织

骨骼肌组织

肝组织

骨骼肌组织

常氧对照组

10

45.04±4.85

1.81±0.42

0.10±0.02

0.19±0.06

缺氧对照组

10

15.16±2.69**

1.46±0.49

0.14±0.05*

0.26±0.06*

白杨素组

10

20.27±4.18 #

1.43±0.40

0.11±0.01

0.17±0.06#

7-HEC组

10

18.89±2.88 #

1.78±0.48

0.10±0.03#

0.18±0.07#
表 1  各组糖原含量和乳酸生成速率比较

组别

n

丙二醛生成速率（nmol·mg–1·min–1）

T-SOD活性（U/mL）

肝组织

骨骼肌组织

肝组织

骨骼肌组织

常氧对照组

10

0.77±0.29

0.48±0.15

2.13±0.11

6.53±0.54

缺氧对照组

10

1.37±0.48**

0.87±0.19**

1.87±0.01**

5.46±0.42**

白杨素组

10

0.81±0.14##

0.50±0.14##

2.52±0.30##

5.20±1.31

7-HEC组

10

0.78±0.28##

0.63±0.11##

2.68±0.12##

6.52±0.96#
表 2  各组丙二醛生成速率和T-SOD活性比较
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