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浙江大学学报(医学版)  2022, Vol. 51 Issue (1): 47-52    DOI: 10.3724/zdxbyxb-2021-0282
专题报道     
西安市与上海市婴儿感染百日咳鲍特菌基因型比较
张娟胜1,林晨2,*(),常玲1,王小强1,魏晓光1,李浩1
1.西安市疾病预防控制中心,陕西 西安 710054
2.上海市浦东新区疾病预防控制中心,上海 200136
Genotypes of Bordetella pertussis isolated from infants in Xi’an and Shanghai
ZHANG Juansheng1,LIN Chen2,*(),CHANG Ling1,WANG Xiaoqiang1,WEI Xiaoguang1,LI Hao1
1. Xi’an Center for Disease Control and Prevention, Xi’an 710054, China;
2. Shanghai Pudong New Area Center for Disease Control and Prevention, Shanghai 200136, China
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摘要:

目的:探讨西安市与上海市临床分离百日咳鲍特菌的基因型特征。方法:收集2018—2019年在西安市和上海市哨点监测医院和社区采集的1岁以下疑似百日咳患儿的鼻咽拭子,经分离培养所得百日咳鲍特菌采用多位点抗原序列分型(MAST)和多位点可变数目串联重复序列分析(MLVA)方法分析其基因型。结果:共采集到疑似百日咳患儿鼻咽拭子1200份,经分离培养得到百日咳鲍特菌60株,其中西安市34株,上海市26株。西安市百日咳鲍特菌的主要MAST型别为prn1/ptxP1/ptxA1/fim3-1/fim2-1(32/34,94.12%),而上海市百日咳鲍特菌的主要MAST型别为prn1/ptxP1/ptxA1/fim3-1/fim2-1(13/26,50.00%)和prn2/ptxP3/ptxA1/fim3-1/fim2-1(11/26,42.31%),两市百日咳鲍特菌MAST型别分布差异有统计学意义(χ2=18.642,P<0.01)。西安市百日咳鲍特菌以MT195(13/34,38.24%)、MT55(10/34,29.41%)、MT104(9/34,26.47%)型别居多,而上海市百日咳鲍特菌以MT27型为主(12/26,46.15%),两市百日咳鲍特菌MLVA型别分布差异有统计学意义(χ2=15.866,P<0.01)。结论:西安市与上海市临床分离婴儿感染百日咳鲍特菌基因型存在差异,应进一步加强中国百日咳鲍特菌基因型演变的监测。

关键词: 百日咳百日咳鲍特菌基因型多位点抗原序列分型多位点可变数目串联重复序列分析    
Abstract:

Objective: To compare the genotypes of Bordetella pertussis isolated from infants in Xi’an and Shanghai. Methods: Samples were collected by nasopharyngeal swab from infants aged <1?year hospitalized with suspected pertussis in Xi’an and Shanghai during 2018 and 2019. Bordetella pertussis was isolated, and multilocus antigen sequence typing (MAST) and multilocus variable-number tandem repeat analysis (MLVA) were used to analyse the genotypes. Results: A total of 1200 samples were collected from infants suspected of pertussis and 60 strains of Bordetella pertussis were isolated, including 34 strains in Xi’an and 26 strains in Shanghai. There were significant differences in the MAST types between Xi’an and Shanghai (χ2=18.642, P<0.01); theprn1/ptxP1/ptxA1/fim3-1/fim2-1 strains dominated in Xi’an (32/34, 94.12%), while the dominated MAST types in Shanghai were prn1/ptxP1/ptxA1/fim3-1/fim2-1 (13/26, 50.00%) and prn2/ptxP3/ptxA1/fim3-1/fim2-1 (11/26, 42.31%). The composition of MLVA type of pertussis strains was also significantly different between Xi’an and Shanghai (χ2=15.866, P<0.01); the MT195 (13/34, 38.24%), MT55 (10/34, 29.41%) and MT104 (9/34, 26.47%) strains dominated in Xi’an, while the MT27 (12/26, 46.15%) strain was most common in Shanghai.Conclusion: There are differences in molecular types of Bordetella pertussis isolated from infants with suspected persussis in Xi’an and Shanghai, indicating that further monitoring of Bordetella pertussis is necessary for better understanding the pathogen evolution in China.

Key words: Pertussis    Bordetella pertussis    Genotype    Multilocus antigen sequence typing    Multilocus variable-number tandem repeat analysis
收稿日期: 2021-09-15 出版日期: 2022-05-17
CLC:  R516.6  
基金资助: 西安市科技计划项目[201805101YX9SF35(2)];西安市卫生科研人才项目(J201801016)
通讯作者: 林晨     E-mail: 247372205@qq.com
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引用本文:

张娟胜,林晨,常玲,王小强,魏晓光,李浩. 西安市与上海市婴儿感染百日咳鲍特菌基因型比较[J]. 浙江大学学报(医学版), 2022, 51(1): 47-52.

ZHANG Juansheng,LIN Chen,CHANG Ling,WANG Xiaoqiang,WEI Xiaoguang,LI Hao. Genotypes of Bordetella pertussis isolated from infants in Xi’an and Shanghai. J Zhejiang Univ (Med Sci), 2022, 51(1): 47-52.

链接本文:

https://www.zjujournals.com/med/CN/10.3724/zdxbyxb-2021-0282        https://www.zjujournals.com/med/CN/Y2022/V51/I1/47

地市

n

疫苗接种情况

疫苗类型

未到接种月龄

未全程免疫

全程免疫

西安市

34

16(47.06)

11(32.35)

7(20.59)

无细胞百白破疫苗(武汉生物制品研究所有限责任公司)

上海市

26

10(38.46)

11(42.31)

5(19.23)

无细胞百白破疫苗(武汉生物制品研究所有限责任公司)、五联疫苗(法国巴斯德生物制药有限公司)、四联疫苗(北京民海生物科技有限公司)

表 1  60株百日咳鲍特菌来源患儿疫苗接种情况一览

MAST型别

西安市

上海市

prn1/ptxP1/ptxA1/fim3-1/fim2-1

32(94.12)

13(50.00)

prn9/ptxP3/ptxA1/fim3-1/fim2-1

1(2.94)

0(0.00)

prn2/ptxP3/ptxA1/fim3-1/fim2-1

1(2.94)

11(42.31)

prn1/ptxP1/ptxA1/fim3-4/fim2-1

0(0.00)

1(3.85)

prn4/ptxP1/ptxA1/fim3-1/fim2-1

0(0.00)

1(3.85)

表 2  西安市与上海市分离百日咳鲍特菌MAST型别比较

MLVA型别

西安市

上海市

MT195

13 (38.24)

4(15.38)

MT104

9(26.47)

3(11.54)

MT55

10(29.41)

6(23.08)

MT27

2(5.88)

12(46.15)

MT10

0(0.00)

1(3.85)

表 3  西安市与上海市分离百日咳鲍特菌MLVA型别分布比较
1 ZHANGQ, ZHENGH, LIUM, et al.The seroepidemiology of immunoglobulin G antibodies against pertussis toxin in China: a cross sectional study[J]BMC Infect Dis, 2012, 12( 1): 138.
doi: 10.1186/1471-2334-12-138
2 BURNSD L, MEADEB D, MESSIONNIERN E. Pertussis resurgence: perspectives from the working group meeting on pertussis on the causes, possible paths forward, and gaps in our knowledge[J]J Infect Dis, 2014, 209( suppl 1): S32-S35.
doi: 10.1093/infdis/jit491
3 张娟胜, 李 芳, 栾 阳, 等. 百日咳鲍特菌23S rRNA位点变异AS-PCR检测方法建立[J]. 中国公共卫生, 2018, 34(6): 923-926
ZHANG Juansheng, LI Fang, LUAN Yang, et al. Development and evaluation of a detection method for 23S rRNA mutation of Bordetella pertussis with allele-specific PCR[J]. Chinese Journal of Public Health, 2018, 34(6): 923-926. (in Chinese)
4 郭丽茹, 张 维, 苏 旭, 等. 2012—2017年天津市百日咳鲍特菌耐药情况分析[J]. 疾病监测, 2018, 33(7): 59-63
GUO Liru, ZHANG Wei, SU Xu, et al. Analysis on drug resistance of Bordetella pertussis isolated in Tianjin[J]. Disease Surveillance, 2018, 33(7): 59-63. (in Chinese)
5 ELOMAAA, HEQ, MINHN N T, et al.Pertussis before and after the introduction of acellular pertussis vaccines in Finland[J]Vaccine, 2009, 27( 40): 5443-5449.
doi: 10.1016/j.vaccine.2009.07.010
6 MOOIF R, VAN LOOI H M, VAN GENTM, et al.Bordetella pertussis strains with increased toxin production associated with pertussis resurgence[J]Emerg Infect Dis, 2009, 15( 8): 1206-1213.
doi: 10.3201/eid1508.081511
7 SAADATIAN-ELAHIM, PLOTKINS, MILLSK H G, et al.Pertussis: biology, epidemiology and prevention[J]Vaccine, 2016, 34( 48): 5819-5826.
doi: 10.1016/j.vaccine.2016.10.029
8 QUEENANA M, CASSIDAYP K, EVANGELISTAA. Pertactin-negative variants of Bordetella pertussis in the United States[J]N Engl J Med, 2013, 368( 6): 583-584.
doi: 10.1056/NEJMc1209369
9 BARKOFFA M, MERTSOLAJ, PIERARDD, et al.Surveillance of circulating Bordetella pertussis strains in Europe during 1998 to 2015[J]J Clin Microbiol, 2018, 56( 5): 01998.
doi: 10.1128/JCM.01998-17
10 XUZ, WANGZ, LUANY, et al.Genomic epidemiology of erythromycin-resistant Bordetella pertussis in China[J]Emerging Microbes Infects, 2019, 8( 1): 461-470.
doi: 10.1080/22221751.2019.1587315
11 张娟胜, 魏晓光, 王小强, 等. 百日咳鲍特菌红霉素耐药株与敏感株的基因型别分析[J]. 中华微生物学和免疫学杂志, 2020, 40(12): 893-897
ZHANG Juansheng, WEI Xiaoguang, WANG Xiaoqiang, et al. Genotype analysis of erythromycin-resistant and -sensitive Bordetella pertussis[J]. Chinese Journal of Microbiology and Immunology, 2020, 40(12): 893-897. (in Chinese)
12 VAN GENTM, HEUVELMANC J, VAN DER HEIDEH G, et al.Analysis of Bordetella pertussis clinical isolates circulating in European countries during the period 1998—2012[J]Eur J Clin Microbiol Infect Dis, 2015, 34( 4): 821-830.
doi: 10.1007/s10096-014-2297-2
13 SCHOULSL M, VAN DER HEIDEH G J, VAUTERINL, et al.Multiple-locus variable-number tandem repeat analysis of dutch Bordetella pertussis strains reveals rapid genetic changes with clonal expansion during the late 1990s[J]J Bacteriol, 2004, 186( 16): 5496-5505.
doi: 10.1128/JB.186.16.5496-5505.2004
14 LITTD J, NEALS E, FRYN K. Changes in genetic diversity of the Bordetella pertussis population in the United Kingdom between 1920 and 2006 reflect vaccination coverage and emergence of a single dominant clonal type[J]J Clin Microbiol, 2009, 47( 3): 680-688.
doi: 10.1128/JCM.01838-08
15 DUQ, WANGX, LIUY, et al.Direct molecular typing of Bordetella pertussis from nasopharyngeal specimens in China in 2012—2013[J]Eur J Clin Microbiol Infect Dis, 2016, 35( 7): 1211-1214.
doi: 10.1007/s10096-016-2655-3
16 FUP, WANGC, TIANH, et al.Bordetella pertussis infection in infants and young children in Shanghai, China, 2016—2017: clinical features, genotype variations of antigenic genes and macrolides resistance[J]Pediatr Infect Dis J, 2019, 38( 4): 370-376.
doi: 10.1097/INF.0000000000002160
17 MIYAJIY, OTSUKAN, TOYOIZUMI-AJISAKAH, et al.Genetic analysis of Bordetella pertussis isolates from the 2008—2010 pertussis epidemic in Japan[J/OL]PLoS One, 2013, 8( 10): e77165.
doi: 10.1371/journal.pone.0077165
18 XUY, TANY, ASOKANATHANC, et al.Characterization of co-purified acellular pertussis vaccines[J]Hum Vaccines ImmunoTher, 2015, 11( 2): 421-427.
doi: 10.4161/21645515.2014.988549
19 LIL, DENGJ, MAX, et al.High prevalence of macrolide-resistant Bordetella pertussis and ptxP1 genotype, mainland China, 2014—2016[J]Emerg Infect Dis, 2019, 25( 12): 2205-2214.
doi: 10.3201/eid2512.181836
20 MOOI F R, VAN LOO I H, KING A J. Adaptation of Bordetella pertussis to vaccination: a cause for its reemergence?[J]. Emerg Infect Dis, 2001, 7(3): 526-528
21 ZHANGL, XUY, ZHAOJ, et al.Effect of vaccination on Bordetella pertussis strains, China[J]Emerg Infect Dis, 2010, 16( 11): 1695-1701.
doi: 10.3201/eid1611.100401
22 WANGZ, LUANY, DUQ, et al.The global prevalence ptxP3 lineage of Bordetella pertussis was rare in young children with the co-purified aPV vaccination: a 5 years retrospective study[J]BMC Infect Dis, 2020, 20( 1): 615.
doi: 10.1186/s12879-020-05332-9
23 XUY, ZHANGL, TANY, et al.Genetic diversity and population dynamics of Bordetella pertuss is in China between 1950—2007[J]Vaccine, 2015, 33( 46): 6327-6331.
doi: 10.1016/j.vaccine.2015.09.040
24 BARTM J, HARRISS R, ADVANIA, et al.Global population structure and evolution of Bordetella pertussis and their relationship with vaccination[J/OL]mBio, 2014, 5( 2): e01074.
doi: 10.1128/mBio.01074-14
25 许雁南, 王晓敏, 周旭东. 杭州市流动人口抗生素知识与行为及其影响因素研究[J].中国社会医学杂志,2021, 38(5): 5
XU Yannan, WANG Xiaomin, ZHOU Xudong. Study on antibiotic-related knowledge, behavior and influencing factors among migrant population in Hangzhou[J]. Chinese Journal of Social Medicine, 2021, 38(5): 5. (in Chinese)
26 杨 琼. 下城区儿童家长抗生素合理使用知识及其影响因素研究[D]. 杭州: 浙江大学, 2018
YANG Qiong. A study on the rational use of antibiotics and its influential factors in children’s parents in Xiacheng district[D]. Hangzhou: Zhejiang University, 2018. (in Chinese)
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