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浙江大学学报（医学版）
浙江大学学报（医学版）  2022, Vol. 51 Issue (1): 47-52    DOI: 10.3724/zdxbyxb-2021-0282
专题报道     
西安市与上海市婴儿感染百日咳鲍特菌基因型比较
张娟胜1,林晨2,*(),常玲1,王小强1,魏晓光1,李浩1
1.西安市疾病预防控制中心，陕西 西安 710054
2.上海市浦东新区疾病预防控制中心，上海 200136
Genotypes of Bordetella pertussis isolated from infants in Xi’an and Shanghai
ZHANG Juansheng1,LIN Chen2,*(),CHANG Ling1,WANG Xiaoqiang1,WEI Xiaoguang1,LI Hao1
1. Xi’an Center for Disease Control and Prevention, Xi’an 710054, China;
2. Shanghai Pudong New Area Center for Disease Control and Prevention, Shanghai 200136, China
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摘要：

目的：探讨西安市与上海市临床分离百日咳鲍特菌的基因型特征。方法：收集2018—2019年在西安市和上海市哨点监测医院和社区采集的1岁以下疑似百日咳患儿的鼻咽拭子，经分离培养所得百日咳鲍特菌采用多位点抗原序列分型（MAST）和多位点可变数目串联重复序列分析（MLVA）方法分析其基因型。结果：共采集到疑似百日咳患儿鼻咽拭子1200份，经分离培养得到百日咳鲍特菌60株，其中西安市34株，上海市26株。西安市百日咳鲍特菌的主要MAST型别为prn1/ptxP1/ptxA1/fim3-1/fim2-1（32/34，94.12%），而上海市百日咳鲍特菌的主要MAST型别为prn1/ptxP1/ptxA1/fim3-1/fim2-1（13/26，50.00%）和prn2/ptxP3/ptxA1/fim3-1/fim2-1（11/26，42.31%），两市百日咳鲍特菌MAST型别分布差异有统计学意义（χ2=18.642，P<0.01）。西安市百日咳鲍特菌以MT195（13/34，38.24%）、MT55（10/34，29.41%）、MT104（9/34，26.47%）型别居多，而上海市百日咳鲍特菌以MT27型为主（12/26，46.15%），两市百日咳鲍特菌MLVA型别分布差异有统计学意义（χ2=15.866，P<0.01）。结论：西安市与上海市临床分离婴儿感染百日咳鲍特菌基因型存在差异，应进一步加强中国百日咳鲍特菌基因型演变的监测。
关键词： 百日咳;  百日咳鲍特菌;  基因型;  多位点抗原序列分型;  多位点可变数目串联重复序列分析    
Abstract:

Objective: To compare the genotypes of Bordetella pertussis isolated from infants in Xi’an and Shanghai. Methods: Samples were collected by nasopharyngeal swab from infants aged <1?year hospitalized with suspected pertussis in Xi’an and Shanghai during 2018 and 2019. Bordetella pertussis was isolated, and multilocus antigen sequence typing (MAST) and multilocus variable-number tandem repeat analysis (MLVA) were used to analyse the genotypes. Results: A total of 1200 samples were collected from infants suspected of pertussis and 60 strains of Bordetella pertussis were isolated, including 34 strains in Xi’an and 26 strains in Shanghai. There were significant differences in the MAST types between Xi’an and Shanghai (χ2=18.642, P<0.01); theprn1/ptxP1/ptxA1/fim3-1/fim2-1 strains dominated in Xi’an (32/34, 94.12%), while the dominated MAST types in Shanghai were prn1/ptxP1/ptxA1/fim3-1/fim2-1 (13/26, 50.00%) and prn2/ptxP3/ptxA1/fim3-1/fim2-1 (11/26, 42.31%). The composition of MLVA type of pertussis strains was also significantly different between Xi’an and Shanghai (χ2=15.866, P<0.01); the MT195 (13/34, 38.24%), MT55 (10/34, 29.41%) and MT104 (9/34, 26.47%) strains dominated in Xi’an, while the MT27 (12/26, 46.15%) strain was most common in Shanghai.Conclusion: There are differences in molecular types of Bordetella pertussis isolated from infants with suspected persussis in Xi’an and Shanghai, indicating that further monitoring of Bordetella pertussis is necessary for better understanding the pathogen evolution in China.
Key words: Pertussis    Bordetella pertussis    Genotype    Multilocus antigen sequence typing    Multilocus variable-number tandem repeat analysis
收稿日期: 2021-09-15 出版日期: 2022-05-17
CLC:  R516.6  
基金资助: 西安市科技计划项目[201805101YX9SF35（2）]；西安市卫生科研人才项目（J201801016）
通讯作者: 林晨     E-mail: 247372205@qq.com
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张娟胜
林晨
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王小强
魏晓光
李浩

引用本文:

张娟胜,林晨,常玲,王小强,魏晓光,李浩. 西安市与上海市婴儿感染百日咳鲍特菌基因型比较[J]. 浙江大学学报（医学版）, 2022, 51(1): 47-52.

ZHANG Juansheng,LIN Chen,CHANG Ling,WANG Xiaoqiang,WEI Xiaoguang,LI Hao. Genotypes of Bordetella pertussis isolated from infants in Xi’an and Shanghai. J Zhejiang Univ (Med Sci), 2022, 51(1): 47-52.

链接本文:

https://www.zjujournals.com/med/CN/10.3724/zdxbyxb-2021-0282    或    https://www.zjujournals.com/med/CN/Y2022/V51/I1/47

地市

n

疫苗接种情况

疫苗类型

未到接种月龄

未全程免疫

全程免疫

西安市

34

16（47.06）

11（32.35）

7（20.59）

无细胞百白破疫苗（武汉生物制品研究所有限责任公司）

上海市

26

10（38.46）

11（42.31）

5（19.23）

无细胞百白破疫苗（武汉生物制品研究所有限责任公司）、五联疫苗（法国巴斯德生物制药有限公司）、四联疫苗（北京民海生物科技有限公司）
表 1  60株百日咳鲍特菌来源患儿疫苗接种情况一览

MAST型别

西安市

上海市

prn1/ptxP1/ptxA1/fim3-1/fim2-1

32（94.12）

13（50.00）

prn9/ptxP3/ptxA1/fim3-1/fim2-1

1（2.94）

0（0.00）

prn2/ptxP3/ptxA1/fim3-1/fim2-1

1（2.94）

11（42.31）

prn1/ptxP1/ptxA1/fim3-4/fim2-1

0（0.00）

1（3.85）

prn4/ptxP1/ptxA1/fim3-1/fim2-1

0（0.00）

1（3.85）
表 2  西安市与上海市分离百日咳鲍特菌MAST型别比较

MLVA型别

西安市

上海市

MT195

13 （38.24）

4（15.38）

MT104

9（26.47）

3（11.54）

MT55

10（29.41）

6（23.08）

MT27

2（5.88）

12（46.15）

MT10

0（0.00）

1（3.85）
表 3  西安市与上海市分离百日咳鲍特菌MLVA型别分布比较
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