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浙江大学学报（医学版）
浙江大学学报（医学版）  2021, Vol. 50 Issue (2): 179-186    DOI: 10.3724/zdxbyxb-2021-0116
专题报道     
卡托普利预处理对自酸蚀粘接剂牙本质粘接持久性的影响
陈亚栋(),罗巧洁,舒畅,李晓军,李晓东()
浙江大学医学院附属口腔医院 浙江大学口腔医学院 浙江省口腔疾病临床医学研究中心 浙江省口腔生物医学重点实验室，浙江 杭州 310006
Effect of captopril pretreatment on the dentin bonding durability of self-etch adhesive
CHEN Yadong(),LUO Qiaojie,SHU Chang,LI Xiaojun,LI Xiaodong()
Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine & Clinical Research Center for Oral Diseases of Zhejiang province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China
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摘要：

目的：通过卡托普利抑制金属基质蛋白酶的活性以提高粘接界面的稳定性。方法：采用不同浓度的卡托普利乙醇溶液和卡托普利乙醇/水溶液对人离体牙牙本质表面进行预处理，通过场发射扫描电镜观察卡托普利预处理对牙本质表面形貌的影响；在此基础上通过两种自酸蚀粘接剂对牙本质进行粘接，表征即刻粘接强度，并通过老化处理，评价卡托普利预处理对粘接持久性的影响。结果：扫描电镜结果提示，卡托普利溶液可以部分去除沾污层并暴露牙本质胶原。基于扫描电镜结果，选择0.15?g/mL卡托普利乙醇溶液（50%乙醇）处理30?s作为预处理条件，通过可乐丽菲露 SE BOND 进行牙本质粘接，即刻微拉伸强度从（30.80±4.70）MPa 提升至（37.48±3.20）MPa（P<0.05）；老化处理 1 年后，对照组微拉伸强度显著下降[（22.90±6.82）MPa，P<0.05]，而实验组微拉伸强度没有明显的变化[（36.56±5.10）MPa，P>0.05]。用可乐丽菲露 S3 BOND 进行牙本质粘接，对照组的即刻微拉伸强度为（31.33±4.11）MPa，与实验组（34.70±4.07）MPa 差异无统计学意义（P>0.05）；老化处理 1 年后，实验组的微拉伸强度高于对照组[分别为（32.36±3.58）MPa和（21.43±6.27）MPa，P<0.05]。结论：卡托普利预处理不仅可以提高自酸蚀粘接的即刻粘接强度，而且改善了粘接的持久性。
关键词： 树脂粘接固定义齿;  牙本质;  持久性;  胶原酶抑制剂;  卡托普利;  混合层    
Abstract:

Objective:To investigate the effect of captopril on the dentin bonding durability of self-etch adhesive. Methods: Different concentrations of captopril ethanol solutions or captopril ethanol/water solutions were prepared to pretreat dentin as primer for the self-etch adhesives. The surface morphology of the dentin was observed with scanning electron microscopy (SEM). Based on the morphology analysis, the pretreatment condition was selected and two self-etch adhesives were employed to evaluate the improvement effect of the captopril pretreatment on the dentin bonding durability. Results: SEM showed that the pretreatment of captopril ethanol solutions and captopril ethanol/water solutions were able to remove the smear lay and partially expose collagen matrix. According to the SEM results, the pretreating condition of 0.15?g/mL captopril ethanol/water solution with the pretreating time of 30?s was selected for further dentin bonding study. For Clearfil SEBOND system, the immediate bonding strength increased from (30.80±4.70)?MPa to (37.48±3.20)?MPa?(P<0.05). After one-year aging, the bonding strength of the control group decreased markedly [(22.90±6.82)?MPa,P<0.05]; while the bonding strength of the captopril pretreated group kept steadily [(36.56±5.10)?MPa,P>0.05]. For Clearfil S3 BOND system, there was no significant difference in the immediate bonding strength between the experimental group [(34.70±4.07)?MPa] and the control group[(31.33±4.11)?MPa]. But after one-year aging, the bonding strength of the experimental group was higher than that of the control group [(32.36±3.58)?MPa and (21.43±6.27)?MPa,P<0.05].Conclusion: The pretreatment with captopril ethanol/water solution increases the dentin bonding strength of the self-etch adhesive systems and also improves the bonding durability.
Key words: Resin-bonded fixed denture    Dentine of tooth    Bonding durability    Collagenase inhibitor    Captopril    Hybrid layer
收稿日期: 2021-01-27 出版日期: 2021-06-18
CLC:  R783.3  
基金资助: “十三五”国家重点研发计划（2018YFC1105302）；浙江省自然科学基金（Y18C100001）；中央高校基本科研业务费专项资金（K20210292）
通讯作者: 李晓东     E-mail: chenyd@zju.edu.cn;cisarli@zju.edu.cn
作者简介: 陈亚栋，主治医师，主要从事牙质粘接机制探索和增强牙质粘接效果的新技术研究；E-mail：chenyd@zju.edu.cn；https：//orcid.org/0000-0002-6125-9441
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引用本文:

陈亚栋,罗巧洁,舒畅,李晓军,李晓东. 卡托普利预处理对自酸蚀粘接剂牙本质粘接持久性的影响[J]. 浙江大学学报（医学版）, 2021, 50(2): 179-186.

CHEN Yadong,LUO Qiaojie,SHU Chang,LI Xiaojun,LI Xiaodong. Effect of captopril pretreatment on the dentin bonding durability of self-etch adhesive. J Zhejiang Univ (Med Sci), 2021, 50(2): 179-186.

链接本文:

http://www.zjujournals.com/med/CN/10.3724/zdxbyxb-2021-0116    或    http://www.zjujournals.com/med/CN/Y2021/V50/I2/179

图 1  卡托普利预处理牙质表面扫描电镜图A：未经卡托普利预处理；B：0.10 g/mL卡托普利乙醇溶液（100%乙醇）处理15 s；C：0.10 g/mL卡托普利乙醇溶液（100%乙醇）处理30 s；D：0.15 g/mL卡托普利乙醇溶液（100%乙醇）处理15 s；E：0.15 g/mL卡托普利乙醇溶液（100%乙醇）处理30 s；F：0.10 g/mL卡托普利乙醇溶液（50%乙醇）处理15 s；G：0.10 g/mL卡托普利乙醇溶液（50%乙醇）处理30 s；H：0.15 g/mL卡托普利乙醇溶液（50%乙醇）处理15 s；I：0.15 g/mL卡托普利乙醇溶液（50%乙醇）处理30 s.
图 2  牙质粘接即刻和 1 年后断裂面分析结果A：用可乐丽菲露 SE BOND 进行牙质粘接；B：用可乐丽菲露 S BOND 进行牙质粘接.
1 BAGRAMIAN R A, ARCIA-GODOY F G, VOLPE A R . The global increase in dental caries. A pending public health crisis[J]. Am J Dent, 2009, 22(1): 3-8
2 DU M Q, LI Z, JIANG H, et al. Dental caries status and its associated factors among 3- to 5-year-old children in China: a national survey[J]. Chin J Dent Res, 2018, 21(3): 167-179
3 QUAN J K, WANG X Z, SUN X Y, et al. Permanent teeth caries status of 12- to 15-year-olds in China: findings from the 4th National Oral Health Survey[J]. Chin J Dent Res, 2018, 21(3): 181-193
4 王　兴. 第四次全国口腔健康流行病学调查报告[M]. 北京: 人民卫生出版社, 2018
WANG Xing. Fourth national oral health epidemiologic survey report [M]. Beijing: People’s Medical Publishing House 2018. (in Chinese)
5 KOPPERUDS E, TVEITA B, GAARDENT, et al.Longevity of posterior dental restorations and reasons for failure[J]Eur J Oral Sci, 2012, 120( 6): 539-548.
doi: 10.1111/eos.12004
6 PASHLEYD H, TAYF R, BRESCHIL, et al.State of the art etch-and-rinse adhesives[J]Dental Mater, 2011, 27( 1): 1-16.
doi: 10.1016/j.dental.2010.10.016
7 BRESCHIL, MAZZONIA, RUGGERIA, et al.Dental adhesion review: aging and stability of the bonded interface[J]Dent Mater, 2008, 24( 1): 90-101.
doi: 10.1016/j.dental.2007.02.009
8 TJ?DERHANEL. Dentin bonding: can we make it last?[J]Oper Dent, 2015, 40( 1): 4-18.
doi: 10.2341/14-095-BL
9 PASHLEYD H, TAYF R, YIUC, et al.Collagen degradation by host-derived enzymes during aging[J]J Dent Res, 2004, 83( 3): 216-221.
doi: 10.1177/154405910408300306
10 LIUY, TJ?DERHANEL, BRESCHIL, et al.Limitations in bonding to dentin and experimental strategies to prevent bond degradation[J]J Dent Res, 2011, 90( 8): 953-968.
doi: 10.1177/0022034510391799
11 BRESCHIL, MARAVICT, CUNHAS R, et al.Dentin bonding systems: from dentin collagen structure to bond preservation and clinical applications[J]Dent Mater, 2018, 34( 1): 78-96.
doi: 10.1016/j.dental.2017.11.005
12 FRASSETTOA, BRESCHIL, TURCOG, et al.Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability—a literature review[J/OL]Dent Mater, 2016, 32( 2): e41-e53.
doi: 10.1016/j.dental.2015.11.007
13 SHAILENDRAM, BHANDARIS, KULKARNIS, et al.Evaluation of indomethacin as matrix metalloproteases inhibitor in human dentin[J]J Conserv Dent, 2019, 22( 6): 598.
doi: 10.4103/jcd.jcd_236_19
14 DAVISR, RIBNERH S, KEUNGE, et al.Treatment of chronic congestive heart failure with captopril, an oral inhibitor of angiotensin-converting enzyme[J]N Engl J Med, 1979, 301( 3): 117-121.
doi: 10.1056/nejm197907193010301
15 PRONTERAC, MARIANIB, ROSSIC, et al.Inhibition of gelatinase A (MMP-2) by batimastat and captopril reduces tumor growth and lung metastases in mice bearing Lewis lung carcinoma[J]Int J Cancer, 1999, 81( 5): 761-766.
doi: 10.1002/(sici)1097-0215(19990531)81:5<761::aid-ijc16>3.0.co;2-1
16 OKADAM, KIKUZUKIR, HARADAT, et al.Captopril attenuates matrix metalloproteinase-2 and-9 in monocrotaline-induced right ventricular hypertrophy in rats[J]J Pharmacol Sci, 2008, 108( 4): 487-494.
doi: 10.1254/jphs.08174FP
17 ZHENGX, HUJ, CHENY, et al.AFM study of the effects of collagenase and its inhibitors on dentine collagen fibrils[J]J Dent, 2012, 40( 2): 163-171.
doi: 10.1016/j.jdent.2011.12.009
18 朱赟婕. 卡托普利和氯己定对牙本质黏结持久性影响的对比研究[D]. 杭州: 浙江大学, 2013
ZHU Yunjie. A comparative study of the effect of captopril and chlorhexidine on the strength and durability of dentin bonding[D]. Hangzhou: Zhejiang University, 2013. (in Chinese)
19 MAZZONIA, PASHLEYD H, NISHITANIY, et al.Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives[J]Biomaterials, 2006, 27( 25): 4470-4476.
doi: 10.1016/j.biomaterials.2006.01.040
20 DE MUNCKJ, VAN DEN STEENP E, MINEA, et al.Inhibition of enzymatic degradation of adhesive-dentin interfaces[J]J Dent Res, 2009, 88( 12): 1101-1106.
doi: 10.1177/0022034509346952
21 AIDAM, ODAKIM, FUJITAK, et al.Degradation-stage effect of self-etching primer on dentin bond durability[J]J Dent Res, 2009, 88( 5): 443-448.
doi: 10.1177/0022034509337146
22 SHOKATIB, TAML E, SANTERREJ P, et al.Effect of salivary esterase on the integrity and fracture toughness of the dentin-resin interface[J]J Biomed Mater Res B Appl Biomater, 2010, 94( 1): 230-237.
doi: 10.1002/jbm.b.31645
23 AFAFK, BOUALEMS, KHACEMK, et al.Experimental analysis of the physical degradation of polymers-the case of polymethyl methacrylate[J]Frattura ed Integrità Strutturale, 2020, 14( 53): 66-80.
doi: 10.3221/igf-esis.53.06
24 MUNCHOWE A, DA SILVAA F, PIVAE, et al.Development of an antibacterial and anti-metalloproteinase dental adhesive for long-lasting resin composite restorations[J]J Mater Chem B, 2020, 8( 47): 10797-10811.
doi: 10.1039/d0tb02058c
25 ZHANGS C, KERNM. The role of host-derived dentinal matrix metalloproteinases in reducing dentin bonding of resin adhesives[J]Int J Oral Sci, 2009, 1( 4): 163-176.
doi: 10.4248/ijos.09044
26 ZHOUW, LIUS Y, ZHOUX D, et al.Modifying adhesive materials to improve the longevity of resinous restorations[J]Int J Mol Sci, 2019, 20( 3): 723.
doi: 10.3390/ijms20030723
27 GIACOMINIM C, SCAFFAP M C, GON?ALVESR S, et al.Profile of a 10-MDP-based universal adhesive system associated with chlorhexidine: dentin bond strength and in situ zymography performance[J]J Mech Behav BioMed Mater, 2020, 103925.
doi: 10.1016/j.jmbbm.2020.103925
28 ZITKAO, KUKACKAJ, KRIZKOVAS, et al.Matrix metalloproteinases[J]Curr Med Chem, 2010, 17( 31): 3751-3768.
doi: 10.2174/092986710793213724
29 DHANARAJV, YEQ Z, JOHNSONL L, et al.X-ray structure of a hydroxamate inhibitor complex of stromelysin catalytic domain and its comparison with members of the zinc metalloproteinase superfamily[J]Structure, 1996, 4( 4): 375-386.
doi: 10.1016/s0969-2126(96)00043-3
30 BODE WMASKOS K . Structural studies on MMPs and TIMPs[J]. Methods Mol Biol, 2001, 151: 45-77
31 JINY, HANH C, LINDSEYM L. ACE inhibitors to block MMP-9 activity: new functions for old inhibitors[J]J Mol Cell Cardiol, 2007, 43( 6): 664-666.
doi: 10.1016/j.yjmcc.2007.09.002
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