Abstract:
Objectives: The goal of this study was to evaluate the modulatory effect of chitosan oligosaccharidenisin
conjugate (CON-C) on intestinal microbiota of human flora-associated (HFA) mice and also reveal
its effect towards the high-fat diet (HFD)-induced obesity. Both Chitosan oligosaccharides and nisin
showed great potential in modulating the intestinal microbiota, so it is worth to explore whether the
modulation effect of chitosan oligosaccharide could be improved by covalently binding with nisin.
Materials and Methods: CON-C was prepared by heating the mixed solution of chitosan
oligosaccharide and nisin at 80°C and pH 2.0 for 24h. The structure of CON-C were analyzed by
Fourier transform-infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). The CON-C’s antiobesity
effect and modulatory effect toward intestinal microbiota were analyzed using human
flora-associated (HFA) mice model.
Results: CON-C could alleviated HFD-induced gut dysbiosis, by significantly decreasing the
numbers of Bifidobacterium and Lactobacillus/Enterococcus spp., and increasing the numbers of
Bacteroides–Prevotella and Clostridium groups. CON-C could also enriched the most differentially
expressed genes through KEGG pathways of biosynthesis of amino acids, two-component system,
and ATP binding cassette (ABC) transporters.
Conclusions: The improved therapeutic effect of CON-C against HFD-induced obesity has been
approved, and hence, CON-C has a great potential to be utilized as a functional food ingredient in
reducing body weight.
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