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浙江大学学报(工学版)
浙江大学学报(工学版)
生物医学工程、化学工程     
低场强脉冲电场杀灭水中大肠杆菌的实验研究
寇艳芹1, 郑超1,2, 徐羽贞1, 黄逸凡1, 刘振1, 闫克平1
1. 浙江大学 生物质化工教育部重点实验室,浙江 杭州 310027；2. 浙江省医疗器械研究所,浙江 杭州 310009
Water disinfection of E. coli by pulsed low electric fields
KOU Yan-qin1, ZHENG Chao1,2, XU Yu-zhen1, HUANG Yi-fan1, LIU Zhen1, YAN Ke-ping1
1. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, China; 2. Zhejiang Institute of Medical Device, Hangzhou 310009, China
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摘要：

采用高频低场强脉冲电场系统对水中的大肠杆菌进行灭菌研究,探索反应器结构、电气参数及水质参数等对脉冲电场灭菌效率和能量效率的影响.圆筒式反应器体积为500 mL,平均脉冲场强为3～12 kV/cm,脉冲频率可以高达20 kHz.最佳高压电极结构为6层均匀分布的直径为40 mm的网电极.脉冲电场的杀菌效率和能量效率随着脉宽和场强的增大而提高,水的电导率影响波形和单脉冲能量的变化,从而影响杀菌效果,当初始细菌密度降低时有利于彻底杀光.脉冲频率对杀菌无明显影响,但高频率能够大幅缩短处理时间.当脉冲电压为6 kV,脉宽为30 μs,水的电导率为2.5 μS/cm,细胞初始密度为103～106 cfu/mL时,在消耗能量<200 J/mL,水温<40 ℃的前提下,脉冲电场处理后细菌密度下降了1、2个对数.当细菌初始密度低于1 250 cfu/mL时,细菌被全部杀光,实现了非热低温灭菌.
Abstract:

Water disinfection of E. coli by high frequency pulsed low electric fields was investigated. The effects of constructional, electrical and aqueous parameters on disinfection efficiency and energy efficiency were evaluated. Experiments were performed with a 500 mL cylindrical reactor. Average electric intensity of 3-12 kV/cm was applied and pulsed frequency was up to 20 000 Hz. Optimized structure of high voltage electrode were six equally distributed mesh electrodes with diameter of 40 mm. Both the disinfection efficiency and energy efficiency were enhanced by increasing the electric strength and pulse duration. Water conductivity could influence the pulsed waveforms and single pulse energy, which changed the disinfection results. Reducing the initial bacterial density made the process more effective and easily realized sterilization. Pulsed frequency did not show any effect on disinfection, but high frequency could greatly reduce treatment time. When pulsed voltage, pulsed width, water conductivity and initial bacteria density were 6 kV, 30 μs, 2.5 μS/cm and 103-106 cfu/mL, respectively, 1-2 logarithmic reduction were obtained with energy consumption less than 200 J/mL and water temperature lower than 40 ℃. If initial bacterial density was less than 1 250 cfu/mL, all the cells could be sterilized, which was a non-thermal processing.
出版日期: 2015-04-01
:  TQ 9  
基金资助:

中央高校科研业务费资助项目(2012XZZX001)
通讯作者: 闫克平,男,教授,博导     E-mail: kyan@zju.edu.cn
作者简介: 寇艳芹（1987—）,女,硕士生,从事生物化工、低温等离子体及生物电工的研究.E-mail: kouyanqin8103@163.com
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寇艳芹, 郑超, 徐羽贞, 黄逸凡, 刘振, 闫克平. 低场强脉冲电场杀灭水中大肠杆菌的实验研究[J]. 浙江大学学报(工学版), 10.3785/j.issn.1008-973X.2015.04.028.

KOU Yan-qin, ZHENG Chao, XU Yu-zhen, HUANG Yi-fan, LIU Zhen, YAN Ke-ping. Water disinfection of E. coli by pulsed low electric fields. JOURNAL OF ZHEJIANG UNIVERSITY (ENGINEERING SCIENCE), 10.3785/j.issn.1008-973X.2015.04.028.

链接本文:

http://www.zjujournals.com/eng/CN/10.3785/j.issn.1008-973X.2015.04.028    或    http://www.zjujournals.com/eng/CN/Y2015/V49/I4/806

［1］ ZHANG Q, BARBOSA-CANOVAS G V, SWANSON B G. Engineering aspects of pulsed electric field pasteurization ［J］. Journal of Food Engineering, 1995, 25(2): 261-281.
［2］ MOSQUEDA-MELGAR J, RAYBAUDI-MASSILIA R M, MARTIN-BELLOSO O. Influence of treatment time and pulse frequency on Salmonella enteritidis, Escherichia coli and Listeria monocytogenes populations inoculated into melon and watermelon juices, treated by pulsed electric field ［J］. International Journal of Food Microbiology, 2007, 117(2): 192-200.
［3］ SAMPEDRO F, RODRIGO D, MARTINEZ A, et al. Review: application of pulsed electric fields in egg and egg derivatives ［J］. Food Science and Technology International, 2006, 12(5): 397-405.
［4］ JAEGER H, SCHULZ A, KARAPETKOV N, et al. Protective effect of milk constituents and sublethal injuries limiting process effectiveness during PEF inactivation of Lb. rhamnosus ［J］. International Journal of Food Microbiology, 2009, 134(1/2): 154-161.
［5］ AGUILO-AGUAYO I, OMS-OLIU G, SOLIVA-FORTUNY R, et al. Flavour retention and related enzyme activities during storage of strawberry juices processed by high-intensity pulsed electric fields or heat ［J］. Food Chemistry, 2009, 116(1): 59-65.
［6］ JIA M, ZHANG Q H, MIN D B. Pulsed electric field processing effects on flavor compounds and microorganisms of orange juice ［J］. Food Chemistry, 1999, 65(4): 445-451.
［7］ HEINZ V, TOEPFL S, KNORR D. Impact of temperature on lethality and energy efficiency of apple juice pasteurization by pulsed electric fields treatment ［J］. Innovative Food Science and Emerging Technologies, 2003, 4(2): 167-175.
［8］ HUANG K, TIAN H, GAI L, et al. A review of kinetic models for inactivating microorganisms and enzymes by pulsed electric field processing ［J］. Journal of Food Engineering, 2012, 111(2): 191-207.
［9］ WOUTERS P C, ALVAREZ I, RASO J. Critical factors determining inactivation kinetic by pulsed electric eld food processing ［J］. Trends in Food Science and Technology, 2001, 12(3/4): 112-121.
［10］ WAN J, COVENTRY J, SWIERGON P, et al. Advances in innovative processing technologies for microbial inactivation and enhancement of food safety-pulsed electric field and low-temperature plasma ［J］. Trands in Food Science and Technology, 2009, 20(9): 414-424.
［11］ SANCHEZ-MORENO C, ANCOS B, PLAZA L, et al. Nutritional approaches and health-related properties of plant foods processed by high pressure and pulsed electric fields ［J］. Critical Reviews in Food Science and Nutrition, 2009, 49(6): 552-576.
［12］ KEMPKES M A, LIANG R, PETRY J, et al. PEF systems for food and waste streams ［C］∥IEEE International Power Modulators and High Voltage Conference. Kas Vegas: IEEE, 2008: 73-76.
［13］ PURTOLAS, LPEZ N, CONDN S, et al. Pulsed electric fields inactivation of wine spoilage yeast and bacteria \[J\]. International Journal of Food Microbiology, 2009, 130(1): 49-55.
［14］ QIN B L, BARBOSA-CANOVAS G V, SWANSON B G, et al. Inactivating microorganism using a pulsed electric field continuous treatment system ［J］. IEEE Transactions on Industry Applications, 1998, 34(1): 43-49.
［15］ LI Y Q, CHEN Q, LIU X H, et al. Inactivation of soybean lipoxygenase in soymilk by pulsed electric fields ［J］. Food Chemistry, 2008, 109(2): 408-414.
［16］ DAMAR S, BOZOGLU F, HIZAL M, et al. Inactivation and injury of Escherichia coli O157: H7 and Staphylococcus aureus by pulsed electric fields ［J］. World Journal of Microbiology, 2002, 18(1): 16
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