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浙江大学学报(工学版)
J4  2009, Vol. 43 Issue (11): 2038-2042    DOI: 10.3785/j.issn.1008-973X.2009.11.017
生物医学工程     
基于磁性纳米线的免疫传感器及检测系统
杨昊1,杨笑鹤2,郭希山3,陈裕泉1,潘敏1
（1.浙江大学 生物传感器国家专业实验室,浙江 杭州 310027；2.浙江省医疗器械研究所,浙江 杭州 310009；3.浙江大学 生物系统工程与食品科学学院,浙江 杭州 310029）
Magnetic nanowires based immunosensor and detecting system
YANG Hao1, YANG Xiao-he2, GUO Xi-shan3, CHEN Yu-quan1, PAN Min1
(1. State Specialized Laboratory of Biomedical Sensors, Zhejiang University, Hangzhou 310027, China;
2. Zhejiang Medical Device Institute, Hangzhou 310009, China;
3. College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China)
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摘要：

为了提高免疫检测速度,实现生物样品的在线分析,研究以磁性纳米线作为标记物的免疫传感器及检测系统.利用磁性纳米线独特的磁化特性,提出一种操作简便、便于携带、响应迅速的磁性免疫检测的具体方案,通过对系统感应线圈几何参数的优化设计,构建磁性纳米线免疫传感器检测系统.利用阳极氧化铝模板,电沉积制备磁性纳米线,并以此合成磁性纳米线/壳聚糖/α-HCG抗体生物探针.对1、2、5 g/L不同质量浓度的样品进行检测,实验结果表明,此检测系统的灵敏度可达0.2 g/L,并且有进一步提高的空间.通过提高激励信号的信噪比、提高线圈加工质量和印制线路板质量、减小布线寄生电容、考虑温度漂移补偿等手段,可以进一步提高检测灵敏度.
Abstract:

The immunosensor and detecting system based on magnetic nanowires were studied for rapid immune detection and on-line analysis of biological sample. Based on the special magnetic properties of magnetic nanowires, an easy operating, easy taking and rapid reacting magnetic detecting system was developed by optimizing the geometry parameters of detect coil. Fe nanowires were electrochemically deposited with porous anodic aluminum oxide template. The biological probes of Fe nanowires/chiosan/α-HCG antibody were synthesized. Samples of concentrations of 1, 2 and 5 g/L were detected. The results showed that the sensitivity of the system was 0.2 g/L. The sensitivity can be improved by increasing the signal noise rate and the quality of coil and printed circuit board, reducing the parasitic capacitance, and compensating the temperature excursion．
出版日期: 2009-11-01
:  R 318  
基金资助:

国家“十一五”科技支撑计划资助项目（2006BAD30B03）;博士后科学基金资助项目(20080441257).
通讯作者: 陈裕泉，男，教授.     E-mail: yqchen@mail.bme.zju.edu.cn
作者简介: 杨昊（1975－），男，四川乐山人，讲师，从事生物传感器及信息处理技术研究.
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引用本文:

杨昊, 杨笑鹤, 郭希山, 等. 基于磁性纳米线的免疫传感器及检测系统[J]. J4, 2009, 43(11): 2038-2042.

YANG Hao, YANG Xiao-He, GUO Xi-Shan, et al. Magnetic nanowires based immunosensor and detecting system. J4, 2009, 43(11): 2038-2042.

链接本文:

http://www.zjujournals.com/eng/CN/10.3785/j.issn.1008-973X.2009.11.017    或    http://www.zjujournals.com/eng/CN/Y2009/V43/I11/2038

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