不同原核表达载体对非洲猪瘟病毒CD2v蛋白可溶性表达及免疫反应性比较

冯梦珂,王星博,林璐璐,崔明仙,颜焰,周继勇

Comparisons on soluble expression and immunoreactivity of African swine fever virus CD2v protein expressed by different prokaryotic expression vectors

Mengke FENG,Xingbo WANG,Lulu LIN,Mingxian CUI,Yan YAN,Jiyong ZHOU

图3 CD2v-TF重组蛋白纯化结果A. SDS-PAGE分析；B.蛋白质印迹法分析。M：蛋白质相对分子质量；1～3：用20 mmol/L咪唑洗脱的杂蛋白；4～6：用50 mmol/L咪唑洗脱的目的蛋白CD2v-TF；7：纯化的目的蛋白CD2v-TF；8：诱导后菌体；9：未诱导菌体（对照）。

Fig. 3 Purification results of CD2v-TF recombinant proteinA. SDS-PAGE analysis; B. Western blotting analysis. M: Relative molecular weight of protein; 1-3: Heterologous proteins eluted with 20 mmol/L imidazole; 4-6: Target protein CD2v-TF eluted with 50 mmol/L imidazole; 7: Purified target protein CD2v-TF; 8: Induced bacteria; 9: Uninduced bacteria (control).