不同原核表达载体对非洲猪瘟病毒CD2v蛋白可溶性表达及免疫反应性比较

冯梦珂,王星博,林璐璐,崔明仙,颜焰,周继勇

Comparisons on soluble expression and immunoreactivity of African swine fever virus CD2v protein expressed by different prokaryotic expression vectors

Mengke FENG,Xingbo WANG,Lulu LIN,Mingxian CUI,Yan YAN,Jiyong ZHOU

图4 CD2v-His重组蛋白纯化结果A. SDS-PAGE分析；B.蛋白质印迹法分析。M：蛋白质相对分子质量；1、7：未诱导菌体（对照）；2、6：诱导后菌体；3：洗脱的杂蛋白；4：洗脱的目的蛋白CD2v-His；5：纯化的目的蛋白CD2v-His。

Fig. 4 Purification results of CD2v-His recombinant proteinA. SDS-PAGE analysis; B. Western blotting analysis. M: Relative molecular weight of protein; 1, 7: Uninduced bacteria (control); 2, 6: Induced bacteria; 3: Eluted heterologous protein; 4: Eluted target protein CD2v-His; 5: Purified target protein CD2v-His.