不同原核表达载体对非洲猪瘟病毒CD2v蛋白可溶性表达及免疫反应性比较

冯梦珂,王星博,林璐璐,崔明仙,颜焰,周继勇

Comparisons on soluble expression and immunoreactivity of African swine fever virus CD2v protein expressed by different prokaryotic expression vectors

Mengke FENG,Xingbo WANG,Lulu LIN,Mingxian CUI,Yan YAN,Jiyong ZHOU

图2 5种不同原核表达载体对CD2v重组蛋白表达的SDS-PAGE分析A～E.分别为pET-28a-CD2vΔSP-TM、pET32a-CD2vΔSP-TM、pGEX-4T-1-CD2vΔSP-TM、pMAL-C6T-CD2vΔSP-TM、pCold-TF-CD2vΔSP-TM重组质粒。M：蛋白质相对分子质量；1：未诱导菌体；2：诱导后菌体；3：诱导后菌体超声裂解上清液；4：诱导后菌体超声裂解沉淀。

Fig. 2 SDS-PAGE analysis of CD2v recombinant protein expression by five different prokaryotic expression vectorsA-E. pET28a-CD2vΔSP-TM, pET32a-CD2vΔSP-TM, pGEX-4T-1-CD2vΔSP-TM, pMAL-C6T-CD2vΔSP-TM and pCold-TF-CD2vΔSP-TM recombinant plasmids, respectively. M: Relative molecular weight of protein; 1: Uninduced bacteria; 2: Induced bacteria; 3: Bacterial supernatant by ultrasonic lysis after induction; 4: Bacterial precipitation by ultrasonic lysis after induction.