不同原核表达载体对非洲猪瘟病毒CD2v蛋白可溶性表达及免疫反应性比较

冯梦珂,王星博,林璐璐,崔明仙,颜焰,周继勇

Comparisons on soluble expression and immunoreactivity of African swine fever virus CD2v protein expressed by different prokaryotic expression vectors

Mengke FENG,Xingbo WANG,Lulu LIN,Mingxian CUI,Yan YAN,Jiyong ZHOU

图1 重组质粒的PCR鉴定结果M：DNA分子标志物；1～5：pET-28a-CD2vΔSP-TM、pET32a-CD2vΔSP-TM、pGEX-4T-1-CD2vΔSP-TM、pMAL-C6T-CD2vΔSP-TM和pCold-TF-CD2vΔSP-TM重组质粒的PCR扩增产物；6：阴性对照。

Fig. 1 PCR identification results of recombinant plasmidsM: DNA marker; 1-5: Amplification products of pET-28a-CD2vΔSP-TM, pET32a-CD2vΔSP-TM, pGEX-4T-1-CD2vΔSP-TM, pMAL-C6T-CD2vΔSP-TM and pCold-TF-CD2vΔSP-TM recombinant plasmids, respectively; 6: Negative control.