脊椎动物神经管背-腹图式形成中形态素的调控作用综述

刘聪,徐鹏飞

Review on regulatory role of morphogens in the formation of dorsal-ventral pattern of vertebrate neural tube

Cong LIU,Pengfei XU

图7 利用Shh浓度梯度体外指导前脑类器官的形成示意图A.利用转录激活因子样效应物核酸酶（TALEN）同源重组技术构建多西环素诱导表达SHH的H9细胞系（iSHH）；B.将iSHH细胞聚集于类器官一端作为信号源以提供Shh浓度梯度；C.在Shh浓度梯度下诱导形成有多种细胞类型的前脑类器官，并再现了体内前脑的细胞图式，包括新皮质、外侧神经节隆起、内侧神经节隆起、下丘脑前叶和下丘脑腹后叶。

Fig. 7 Schematic description of applying Shh concentration gradient to instruct forebrain organoid formation in vitroA. A doxycycline-inducible Shh H9 cell line (iSHH) was generated using transcription activator-like effector nucleases (TALEN) homologous recombination technology; B. iSHH cells were aggregated and fused to one side of the organoid to establish the Shh concentration gradient; C. By exposed to different concentrations of Shh, a forebrain organoid was induced and could recapitulate the topography of the in vivo forebrain, which included multiple cell types like neocortex, lateral ganglionic eminence, medial ganglionic eminence, anterior hypothalamus, and ventro-posterior hypothalamus.